Continuous flow mucosal cells for measuring the in-vitro permeability of small tissue samples.
J Pharm Sci
; 86(1): 82-4, 1997 Jan.
Article
em En
| MEDLINE
| ID: mdl-9002464
ABSTRACT
Continuous-flow chambers are described for the measurement of permeability of small tissue samples. The design incorporates a large-capacity donor chamber to permit adequate loading of the applied compound and a low-volume (0.3 mL) receiving chamber that ensures rapid removal of penetrant at relatively low (1.5 mL/h or less) pumping rates. Different sized support disks allow tissue biopsies as small as 4 mm in diameter to be utilized. Comparisons of flux and permeability constants (Kp) for water across oral mucosa indicate that there was no significant difference between values obtained for 10- and 4-mm biopsies. Comparisons of flux and Kp values for porcine oral mucosa and a synthetic membrane between continuous flow and conventional, side-by-side chambers indicated that the latter values were significantly lower, suggesting stasis and inefficient removal of perfusate in the side-by-side design. The Kp values for water obtained in the continuous-flow chambers with pig skin were similar to those published elsewhere for human skin.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Mucosa Bucal
Limite:
Animals
Idioma:
En
Revista:
J Pharm Sci
Ano de publicação:
1997
Tipo de documento:
Article