[Characterization of rat lines with normotensive and hypertensive status using genomic fingerprinting]. / Kharakteristika linii krys s normotenzivnym i gipertenzivnym statusom metodom genomnogo fingerprintinga.

ABSTRACT

The properties of normotensive and hypertensive rat lines were investigated by the DNA fingerprinting method using a multilocus micro-satellite (CAC)5 probe. The HaeIII and HinfI restriction endonucleases were found to be the most informative enzymes in this case. The high genetic homogeneity of the ISIAH line, a rat line with inherited stress-sensitive arterial hypertension created at the Institute of Cytology and Genetics, was demonstrated. The lack of intralinear polymorphism was also typical to Japanese SHR rats with spontaneous arterial hypertension. Normotensive WAG rats had identical fingerprinting patterns, while the relative intensity of some bands was different. The outbred normotensive Wistar line maintained at the Institute, appeared to carry 30% polymorphic alleles. Hypertensive lines differed from the normotensive by a number of genetic markers.