A chemoenzymatic synthesis of UDP-(2-deoxy-2-fluoro)-galactose and evaluation of its interaction with galactosyltransferase.
Bioorg Med Chem
; 5(3): 497-500, 1997 Mar.
Article
em En
| MEDLINE
| ID: mdl-9113327
ABSTRACT
Uridine 5'-diphospho-(2-deoxy-2-fluoro)galactose (UDP-2FGal), prepared and characterized for the first time by a chemoenzymatic method, was found to be a competitive inhibitor of beta-1,4-galactosyltransferase with a Ki value of 149 microM. This study supports that the glycosyltransferase reaction mechanism proceeds through a glycosidic cleavage transition state with sp2 character developed at the anomeric center.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Uridina Difosfato Galactose
/
N-Acetil-Lactosamina Sintase
/
Inibidores Enzimáticos
Idioma:
En
Revista:
Bioorg Med Chem
Ano de publicação:
1997
Tipo de documento:
Article