One-electron photo-oxidation of reduced Desulfovibrio vulgaris flavodoxin on laser excitation at 355 nm.
Biochim Biophys Acta
; 1339(1): 97-100, 1997 Apr 25.
Article
em En
| MEDLINE
| ID: mdl-9165104
ABSTRACT
Electron ejection from the reduced flavin in flavodoxin from Desulfovibrio vulgaris was obtained on exposure of the protein to the third harmonic radiation (354.7 nm) generated from a pulsed Nd/YAG laser. The results indicate that the reaction is due to stepwise two-photon excitation of the reduced flavin via the excited singlet state. The absorption spectrum of the neutral flavosemiquinone radical formed in this process was obtained. This spectrum remains stable over the time of study (0.2 ms) in the pH range studied, except for a slight evolution during the first microseconds, attributed to conformational readjustments of the active site. This two-photon excitation method provides a convenient means of generating the flavosemiquinone for ultrafast kinetic studies.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Desulfovibrio vulgaris
/
Flavodoxina
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1997
Tipo de documento:
Article