Definition of essential amino acid residues in the recognition of a peptide by a mouse monoclonal antibody.
Z Naturforsch C J Biosci
; 52(3-4): 274-8, 1997.
Article
em En
| MEDLINE
| ID: mdl-9167279
ABSTRACT
A mouse monoclonal antibody reacting in ELISA with a synthetic peptide representing a linear amino acid stretch of the protein antigen was tested on all overlapping 5-mer to 9-mer fragments of the peptide, as prepared by multi-pin synthesis. Analysis of the binding data suggests that several residues in the peptide might be relatively unrelevant for recognition, while few others seem to play a critical role as key residues. On the basis of such observations, we attempted to reconstruct an alternative essential epitope by introducing multiple amino acid substitutions in the 9-mer peptide exhibiting the best binding activity, and then tested its ability to be recognized by the monoclonal antibody.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos Monoclonais
/
Epitopos
Limite:
Animals
Idioma:
En
Revista:
Z Naturforsch C J Biosci
Ano de publicação:
1997
Tipo de documento:
Article