Complete cDNAs for CDC42 from chicken cochlea and mouse liver.

ABSTRACT

CDC42 is a member of the ras superfamily of small GTP-binding proteins that are related through the highly conserved GTP-binding domain and are involved in signal transduction pathways. Two full-length CDC42 cDNAs have been isolated a 2148-bp chick cochlea cDNA and a 2063-bp mouse liver cDNA. Each encodes a CDC42 protein of 191 amino acids. The avian CDC42 protein differs from the mouse at only one amino acid residue, a Thr for a Ser at position 185. Both CDC42 proteins are more similar to the ubiquitous human isoform originally isolated from placenta than to the isoform isolated from fetal brain. Using a probe from the 3' UTR of the mouse liver CDC42 cDNA, we demonstrated that the mouse gene is expressed in all tissues examined. Southern blot analysis of a mouse inter-specific backcross with this gene-specific probe identified at least three CDC42-like (Cdc42l) genes in the mouse genome. Cdc42l1 was mapped to distal mouse Chromosome 4, near Cappb1. Cdc42l2 mapped more proximal on Chromosome 4, whereas Cdc42l3 mapped to the X Chromosome.