Serogrouping and topotyping of Sudanese and United States strains of epizootic hemorrhagic disease virus using PCR.
Comp Immunol Microbiol Infect Dis
; 20(3): 211-8, 1997 Jun.
Article
em En
| MEDLINE
| ID: mdl-9280388
ABSTRACT
The potential use of the recently reported polymerase chain reaction (PCR) protocol for detection of United States epizootic hemorrhagic disease virus (EHDV) serotype 1 (EHDV-1) and serotype 2 (EHDV-2) ribonucleic acid in cell culture and clinical specimens was evaluated for detection of Sudanese EHDV strains. EHDV serotype 5 (EHDV-5) and EHDV, isolate 318 (untyped) designated (EHDV-318), recovered from sentinel calves at the Khartoum University farm (Sudan) were studied. RNA from EHDV-5 and EHDV-318 and a number of EHDV field isolates, propagated in cell cultures, were detected by the described PCR-based assay. The specific 387 bp PCR products were visualized on ethidium-bromide stained agarose gel. Specificity of the PCR products was confirmed by chemiluminescent hybridization with non-radiolabeled internal probe. Amplification product was not detected when the PCR-based assay was applied to RNA from blutongue virus (BTV) prototypes serotypes 2, 10, 11, 13, 16 and 17; total nucleic acid extracts from uninfected BHK-21 cells. The results of this study indicated that the previously described EHDV-PCR assay could be applied for detection of Sudanese as well as United States strains of EHDV serogroup. In addition, the described EHDV-PCR assay could be used as a supportive diagnostic assay to the current conventional virus isolation procedures used for detection of EHDV infection in susceptible ruminants.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cervos
/
Reação em Cadeia da Polimerase
/
Vírus da Doença Hemorrágica Epizoótica
/
Infecções por Reoviridae
Tipo de estudo:
Diagnostic_studies
/
Guideline
Limite:
Animals
País/Região como assunto:
Africa
/
America do norte
Idioma:
En
Revista:
Comp Immunol Microbiol Infect Dis
Ano de publicação:
1997
Tipo de documento:
Article