The gene coding for the DOPA dioxygenase involved in betalain biosynthesis in Amanita muscaria and its regulation.
Mol Gen Genet
; 256(1): 1-6, 1997 Sep.
Article
em En
| MEDLINE
| ID: mdl-9341673
ABSTRACT
Genomic and cDNA clones derived from the gene (dodA) coding for DOPA dioxygenase, a key enzyme in the betalain pathway, were obtained from the basidiomycete Amanita muscaria. A cDNA library was established in the phage lambda ZapII and dodA clones were isolated using polyclonal antibodies raised against the purified enzyme. Their identity was confirmed by comparison of the deduced amino acid sequence with the sequence of several tryptic peptide fragments of DOPA dioxygenase. The gene coded for a 228-amino acid protein that showed no homology to published sequences. The coding region was interrupted by five short introns. Regulation was shown to occur at the transcriptional level; the mRNA accumulated to high levels only in the coloured cap tissue. dodA was found to be a single-copy gene in A. muscaria. To our knowledge, this is the first gene from the betalain pathway to be cloned. It encodes a type of aromatic ring-cleaving dioxygenase that has not been previously described.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxigenases
/
Pigmentos Biológicos
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Proteínas Fúngicas
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Di-Hidroxifenilalanina
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Regulação Fúngica da Expressão Gênica
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Amanita
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Genes Fúngicos
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Compostos de Amônio Quaternário
Idioma:
En
Revista:
Mol Gen Genet
Ano de publicação:
1997
Tipo de documento:
Article