Two-dimensional distribution of Gi2alpha in the plasma membrane: a critical evaluation by immunocytochemistry.
FEBS Lett
; 415(2): 139-44, 1997 Sep 29.
Article
em En
| MEDLINE
| ID: mdl-9350984
ABSTRACT
Caveolae have been postulated as a center for signal transduction, because many signaling molecules are concentrated in caveolin-rich fractions. We took Gi2alpha as an example and examined whether it is constitutively concentrated in caveolae. First, the behavior of caveolin and Gi2alpha in density-equilibrium ultracentrifugation was reexamined. By collecting fractions efficiently, caveolin and Gi2alpha were found to distribute differently. Secondly, by novel immunocytochemical methods it was found that the labeling density of Gi2alpha was 2.29 times higher in caveolae than in the non-caveolar plasma membrane. The results indicate that the concentration of Gi2alpha in caveolae is lower than deduced from most biochemical studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Proteínas Proto-Oncogênicas
/
Proteínas de Ligação ao GTP
/
Caveolinas
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1997
Tipo de documento:
Article