Direct PCR amplification and sequence analysis of extrachromosomal Plasmodium DNA from dried blood spots.
Acta Trop
; 68(1): 105-14, 1997 Oct 14.
Article
em En
| MEDLINE
| ID: mdl-9352006
ABSTRACT
The Plasmodium parasite possesses two extrachromosomal genomes; the mitochondrial genetic element and the extrachromosomal plastid-like DNA. The latter has only been fully described for one culture strain of P. falciparum. In this study, a rapid procedure for amplifying plastid DNA from dried blood spots of blood infected with different malaria species was developed. PCR amplification of a 595 bp fragment within the plastid-like large subunit ribosomal-RNA (LSU-rRNA) gene was achieved using primers derived from the P. falciparum sequence. The PCR product was observed in all Plasmodium species examined. Sequence analysis of amplified products homologous to an LSU-rRNA fragment of the plastid-like extrachromosomal circle revealed extensive conservation between Plasmodium species including P. falciparum, P. vivax, P. malariae and P. berghei.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Plasmodium
/
Reação em Cadeia da Polimerase
/
DNA de Protozoário
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Acta Trop
Ano de publicação:
1997
Tipo de documento:
Article