Purification and gas chromatographic-mass spectrometric characterization of non-methylene interrupted fatty acid incorporated in rat liver.
J Chromatogr B Biomed Sci Appl
; 700(1-2): 1-8, 1997 Oct 24.
Article
em En
| MEDLINE
| ID: mdl-9390709
ABSTRACT
A C20 non-methylene interrupted trienoic acid detected in the liver of rat fed with a pine (Pinus koraiensis) seed oil diet was purified by two-step argentation thin-layer chromatography (AgTLC) and characterized by gas chromatography-mass spectrometry (GC-MS). First, a C20 methyl trienoate fraction was obtained from fatty acid methyl esters prepared from rat liver by 5% AgTLC developed with petroleum ether-diethyl ether-acetic acid (70202, v/v) as a solvent system. The fraction was then subjected to AgTLC developed with benzene-acetone-diethyl ether-acetic acid (6515155, v/v) which could separate non-methylene interrupted fatty acids (NMIFA) from usual MIFAs. The purified C20 NMIFA was partially hydrogenated, and the resulting three kinds of the C20 monoenoate were analyzed by GC-MS after conversion to their dimethyl disulfide (DMDS) adducts. The results revealed that the original C20 non-methylene interrupted trienoic acid detected in the liver of rats fed with a pine seed oil diet was delta-5,11,14/203, a minor component of pine seed oil.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Graxos Insaturados
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Ano de publicação:
1997
Tipo de documento:
Article