Characteristics of the myocardial PM-FABP: effect of diabetes mellitus.
Mol Cell Biochem
; 176(1-2): 281-6, 1997 Nov.
Article
em En
| MEDLINE
| ID: mdl-9406173
Properties of the myocardial PM-FABP were studied in normal and STZ-diabetic rats. The fluorescent fatty acids trans-parinaric and cis-parinaric acids were used as analogs of straight-chain (saturated) and kinked-chain (unsaturated) fatty acids respectively. Parinaric acid binding was sensitive to trypsin. Trans-parinaric acid binding was more sensitive to this protease than the binding of cis-parinaric acid. Based on the difference in sensitivity of parinaric acid binding we believe that there are two separate binding sites associated with myocardial PM-FABP; one for unsaturated fats and the other for saturated fats. Diabetes enhanced both cis- and trans-parinaric acid binding capacity in cardiomyocytes; cis-parinaric acid by 2 fold and trans-parinaric acid by 2.6 fold. In addition, there was a concomitant accumulation of free fatty acids and triglycerides in the hearts of the diabetic animals. There was a 2.2 fold increase for fatty acids and a 1.6 fold increase for trigylcerides. This association between myocardial fatty acid build-up and enhanced myocardial PM-FABP during diabetes suggest that this carrier protein might have contributed to lipid accumulation in the hearts of the diabetic rats.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Proteína P2 de Mielina
/
Diabetes Mellitus Experimental
/
Cardiomiopatias
/
Miocárdio
/
Proteínas de Neoplasias
/
Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biochem
Ano de publicação:
1997
Tipo de documento:
Article