Polyamines may regulate S-phase progression but not the dynamic changes of chromatin during the cell cycle.
J Cell Biochem
; 68(2): 200-12, 1998 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-9443076
ABSTRACT
Several studies suggest that polyamines may stabilize chromatin and play a role in its structural alterations. In line with this idea, we found here by chromatin precipitation and micrococcal nuclease (MNase) digestion analyses, that spermidine and spermine stabilize or condense the nucleosomal organization of chromatin in vitro. We then investigated the possible physiological role of polyamines in the nucleosomal organization of chromatin during the cell cycle in Chinese hamster ovary (CHO) cells deficient in ornithine decarboxylase (ODC) activity. An extended polyamine deprivation (for 4 days) was found to arrest 70% of the odc- cells in S phase. MNase digestion analyses revealed that these cells have a highly loosened and destabilized nucleosomal organization. However, no marked difference in the chromatin structure was detected between the control and polyamine-depleted cells following the synchronization of the cells at the S-phase. We also show in synchronized cells that polyamine deprivation retards the traverse of the cells through the S phase already in the first cell cycle. Depletion of polyamines had no significant effect on the nucleosomal organization of chromatin in G1-early S. The polyamine-deprived cells were also capable of condensing the nucleosomal organization of chromatin in the S/G2 phase of the cell cycle. These data indicate that polyamines do not regulate the chromatin condensation state during the cell cycle, although they might have some stabilizing effect on the chromatin structure. Polyamines may, however, play an important role in the control of S-phase progression.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliaminas
/
Cromatina
/
Ciclo Celular
/
Fase S
Limite:
Animals
Idioma:
En
Revista:
J Cell Biochem
Ano de publicação:
1998
Tipo de documento:
Article