Tissue-specific chromatin structure at the hepatocyte growth factor/scatter factor gene promoter.
Gene
; 211(1): 141-50, 1998 Apr 28.
Article
em En
| MEDLINE
| ID: mdl-9573349
ABSTRACT
Hepatocyte growth factor/scatter factor (HGF/SF) is a recently characterised molecule with many remarkable functions. Its involvement in important processes such as cell proliferation, cell migration, morphogenesis and organ development implies that its activity should be tightly regulated. To understand the molecular mechanisms controlling HGF/SF transcription, we have analysed DNaseI hypersensitive sites (DHS) along rat and human HGF/SF genes in various tissues and cell types. We identified five DHS along the rat gene, two in the 5'-flanking region and three in the first intron. These sites are only found in rat tissues and rat cell lines, which express HGF/SF. The strongest hypersensitive site map to a region that corresponds to the promoter by start site analysis. A single tissue-specific DHS is present in human cell lines that express HGF/SF and corresponds to the promoter region. Our results suggest that chromatin accessibility plays a major role in the regulation of HGF/SF transcription regulation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Regiões Promotoras Genéticas
/
Interleucina-3
/
Fator de Crescimento de Hepatócito
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Gene
Ano de publicação:
1998
Tipo de documento:
Article