Effect of flow direction on the morphological responses of cultured bovine aortic endothelial cells.
Med Biol Eng Comput
; 36(1): 122-8, 1998 Jan.
Article
em En
| MEDLINE
| ID: mdl-9614760
ABSTRACT
The effect of flow direction on the morphology of cultured bovine aortic endothelial cells is studied. Fully confluent endothelial cells cultured on glass were subjected to a fluid-imposed shear stress of 2 Pa for 20 min and 24 h using a parallel plate flow chamber. Experiments on shear flow exposure were performed for (i) one-way flow, (ii) reciprocating flow with a 30 min interval and (iii) alternating orthogonal flows with a 30 min interval. After flow exposure, the endothelial cells were fixed and F-actin filaments were stained with rhodamine phalloidin. Endothelial cells were observed and photographed by means of a microscope equipped with epifluorescence optics. The shape index (SI) and angle of cell orientation were measured, and F-actin distributions in the cells were statistically studied. Endothelial cells under the one-way flow condition showed marked elongation (SI = 0.39 +/- 0.16, mean +/- S.D.) and aligned with the flow direction. In the case of the reciprocating (SI = 0.63 +/- 0.14) and the alternating orthogonal flows (0.64 +/- 0.14), cells did not elongate so strongly as in the case of one-way flow. Although most cells in the reciprocating flow aligned with the flow direction, the cell axes in the alternate orthogonal flow distributed around a mean value of -45.1 degrees with a large S.D. value. Endothelial cells can be expected to recognise the flow direction, and change their shape and F-actin structure.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endotélio Vascular
Limite:
Animals
Idioma:
En
Revista:
Med Biol Eng Comput
Ano de publicação:
1998
Tipo de documento:
Article