Determination of pilocarpine, isopilocarpine, pilocarpic acid and isopilocarpic acid in human plasma and urine by high-performance liquid chromatography with tandem mass spectrometric detection.
J Chromatogr B Biomed Sci Appl
; 708(1-2): 103-12, 1998 Apr 24.
Article
em En
| MEDLINE
| ID: mdl-9653952
ABSTRACT
A method is described for the determination of pilocarpine and its degradation products isopilocarpine, pilocarpic acid and isopilocarpic acid in human plasma and urine. The method is based on a simple sample preparation step -- ultrafiltration for plasma and dilution for urine samples -- followed by a reversed-phase liquid chromatographic separation of the analytes and detection by means of tandem mass spectrometry. Parameters affecting the performance of these steps are discussed. The high sensitivity and selectivity of the method allow low ng/ml concentrations to be determined for all compounds in plasma and undiluted urine, which enables the investigation of the metabolic fate and elimination of pilocarpine after oral administration to humans.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pilocarpina
/
Espectrometria de Massas
/
Cromatografia Líquida de Alta Pressão
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Ano de publicação:
1998
Tipo de documento:
Article