Rapid detection of gammaT cell receptor gene rearrangements in acute lymphoblastic leukemia by electrophoresis and silver staining: implications for detection of minimal residual disease.
Electrophoresis
; 19(8-9): 1385-7, 1998 Jun.
Article
em En
| MEDLINE
| ID: mdl-9694286
ABSTRACT
Minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL) was studied using polymerase chain reaction (PCR). GammaT cell receptor (TCRG) genes are ideal targets for PCR-based detection of MRD due to their molecular characteristics. Polyacrylamide gel electrophoresis (PAGE) analysis of PCR products followed by silver staining was performed for 72 children with ALL at the onset of disease. Silver staining is an effective technique to detect gene rearrangements without the use of ethidium bromide. Moreover, this method may show heteroduplex bands of a clonal nature when both TCRG alleles are rearranged. PCR products subjected to a rapid staining protocol were recovered from the gel, reamplified by a second PCR and directly sequenced. After sequencing, we identified the junctional region and obtained patient-specific probes. In more than half of the patients we detected TCRG rearrangements that were used as molecular markers for residual disease.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rearranjo Gênico do Linfócito T
/
Reação em Cadeia da Polimerase
/
Coloração pela Prata
/
Receptores de Antígenos de Linfócitos T gama-delta
/
Neoplasia Residual
/
Eletroforese em Gel de Poliacrilamida
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Child
/
Humans
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
1998
Tipo de documento:
Article