Assessing the functionality of a membrane protein in a three-dimensional crystal.
J Mol Biol
; 281(4): 587-92, 1998 Aug 28.
Article
em En
| MEDLINE
| ID: mdl-9710532
ABSTRACT
Hexagonal microcrystals of bacteriorhodopsin embedded in a lipidic cubic phase have been investigated by time-resolved FT-IR and resonance Raman spectroscopy. Retinal isomerization, conformational changes in the protein backbone and proton translocation are virtually indistinguishable from those in the native membrane. The protein is thus fully active in three-dimensional crystals.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacteriorodopsinas
/
Proteínas de Membrana
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
1998
Tipo de documento:
Article