Crystal structure of an eight-base pair duplex containing the 3'-DNA-RNA-5' junction formed during initiation of minus-strand synthesis of HIV replication.
Biochemistry
; 37(35): 12005-11, 1998 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-9724510
ABSTRACT
During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
DNA Viral
/
RNA Viral
/
HIV-1
/
Conformação de Ácido Nucleico
/
Ácidos Nucleicos Heteroduplexes
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biochemistry
Ano de publicação:
1998
Tipo de documento:
Article