Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.
J Bacteriol
; 180(18): 4967-73, 1998 Sep.
Article
em En
| MEDLINE
| ID: mdl-9733705
ABSTRACT
The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed that dacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor sigmaH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacC insertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression of dacC in Escherichia coli showed that this gene encodes an approximately 59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Proteínas de Bactérias
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Muramilpentapeptídeo Carboxipeptidase
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Proteínas de Transporte
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Peptidil Transferases
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Genes Bacterianos
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Hexosiltransferases
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
1998
Tipo de documento:
Article