Determination of the molecular species composition of diacylglycerols in human adipose tissue by solid-phase extraction and gas chromatography on a polar phase.
J Chromatogr B Biomed Sci Appl
; 714(2): 127-32, 1998 Sep 04.
Article
em En
| MEDLINE
| ID: mdl-9766852
ABSTRACT
The free diacylglycerols (DAGs) in adipose tissue are involved in the metabolism of stored lipids and hence are related to the supply of fatty acids for other tissues. This paper describes a simple, fast, and reproducible method for the identification and quantification of different molecular species of DAGs in human adipose tissue. The method comprised solid-phase extraction on a diol-bonded phase column combined with capillary GC analysis of silylated DAG derivatives on a polar phase (65% phenylmethylsilicone). Separation of the DAGs was achieved based on chain length, isomeric structure (1,2- and 1,3-DAGs), and degree of unsaturation. The main DAGs were 1,2-OO, 1,2-OP, 1,2-LO and 1,2-LP. The composition was corroborated by analysis of the component fatty acids of the DAGs, 181(n-9), 160, and 182(n-6) being the three major fatty acids obtained.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tecido Adiposo
/
Cromatografia Gasosa
/
Cromatografia em Camada Fina
/
Diglicerídeos
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Ano de publicação:
1998
Tipo de documento:
Article