Quantitation of vancomycin and its crystalline degradation product (CDP-1) in human serum by high performance liquid chromatography.
J Pharm Biomed Anal
; 16(8): 1281-7, 1998 Apr.
Article
em En
| MEDLINE
| ID: mdl-9777601
ABSTRACT
The delayed clearance of vancomycin results in accumulation of vancomycin crystalline degradation product, CDP-1, in the bodies of renally impaired patients. The 2 isomers, CDP-1-M (major) and CDP-1-m (minor), of CDP-1 are antibiotically inactive but cross-react with some immunoassays that use polyclonal antibodies resulting in falsely elevated results. A high performance liquid chromatographic (HPLC) method was developed to quantitate vancomycin and CDP-1 in the serum of renal patients. After solid phase extraction of 200 microliters serum, the separation of vancomycin, the 2 isomers of CDP-1 and the internal standard (cefazolin) was accomplished by gradient HPLC on a reversed phase C18 column with detection at 210 nm. Linearity was established from 1 to 25 and 25 to 100 micrograms ml-1 vancomycin and 1 to 25 micrograms ml-1 CDP-1. Coefficients of variation for vancomycin and CDP-1 were 3.3-8.6% (n = 10) and 2.8-5.2% (n = 8).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vancomicina
/
Cromatografia Líquida de Alta Pressão
/
Nefropatias
Limite:
Humans
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
1998
Tipo de documento:
Article