Overproduction of the Bradyrhizobium japonicum c-type cytochrome subunits of the cbb3 oxidase in Escherichia coli.
Biochem Biophys Res Commun
; 251(3): 744-7, 1998 Oct 29.
Article
em En
| MEDLINE
| ID: mdl-9790980
ABSTRACT
We report on a system to improve expression of mature c-type cytochromes in Escherichia coli. It is based on the use of plasmid pEC86 that expresses the E. coli cytochrome c maturation genes ccmABCDEFGH constitutively, whereby the production of both endogenous and foreign c-type cytochromes was increased substantially. The periplasmic soluble domains of the c-type cytochrome subunits FixO and FixP of the Bradyrhizobium japonicum cbb3 oxidase could be expressed in E. coli only when pEC86 was provided in a degP-deficient strain. This shows that a stimulation of heme attachment by the Ccm maturase system combined with the diminished proteolytic activity in the periplasm can increase c-type cytochrome yields.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Proteínas Recombinantes
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Bradyrhizobium
/
Grupo dos Citocromos c
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1998
Tipo de documento:
Article