Citrate uptake into tonoplast vesicles from acid lime (Citrus aurantifolia) juice cells.
J Membr Biol
; 166(3): 197-203, 1998 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-9843593
ABSTRACT
Citrate transport into the vacuoles of acid lime juice cells was investigated using isolated tonoplast vesicles. ATP stimulated citrate uptake in the presence or in the absence of a Delta mu H+. Energization of the vesicles only by an artificial K+ gradient (establishing an inside-positive Delta psi) also resulted in citrate uptake as was the case of a Delta pH dominated Delta mu H+. Addition of inhibitors to endomembrane ATPases showed no direct correlation between the inhibition to the tonoplast bound H+/ATPase and citrate uptake. The data indicated that, although some citrate uptake can be accounted for by Delta psi and by a direct primary active transport mechanism involving ATP, under in vivo conditions of vacuolar pH of 2.0, citrate uptake is driven by Delta pH.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacúolos
/
Trifosfato de Adenosina
/
Citratos
/
Citrus
/
Plastídeos
Idioma:
En
Revista:
J Membr Biol
Ano de publicação:
1998
Tipo de documento:
Article