Truncated TSG101 transcripts are present in peripheral blood from both familial breast cancer patients and controls.
Eur J Cancer
; 34(7): 1077-80, 1998 Jun.
Article
em En
| MEDLINE
| ID: mdl-9849457
ABSTRACT
TSG101 is a recently identified putative tumour suppressor gene which has been implicated in human breast cancer. To address whether germline disruption of TSG101 predisposes individuals to this disease, we analysed genomic DNA and mRNA isolated from peripheral blood from 20 familial breast cancer cases. No evidence of large intragenic insertions/deletions or point mutations in TSG101 was found by Southern blot analysis and sequence analysis of the entire coding region. However, in 11 of 20 samples, 'aberrant' transcripts were detected. Sequence analysis suggested that these variants were generated by the use of different cryptic splicing sites. Such alternative/aberrant splicing events were not restricted to cancer patients, but were also detected in peripheral blood of non-cancer patients and in normal tissues.
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Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Síndromes Neoplásicas Hereditárias
/
Neoplasias da Mama
/
DNA de Neoplasias
/
Genes Supressores de Tumor
/
Proteínas de Ligação a DNA
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Eur J Cancer
Ano de publicação:
1998
Tipo de documento:
Article