Production and characterisation of monoclonal antibodies specific for bovine interleukin-4.
Vet Immunol Immunopathol
; 66(2): 99-112, 1998 Nov 24.
Article
em En
| MEDLINE
| ID: mdl-9860184
ABSTRACT
Genetic immunisation is a simple method for producing polyclonal antibodies in mice. By this method, we produced antibodies against bovine interleukin-4 (BoIL-4). After a final injection with a recombinant BoIL-4 protein, nine stable hybridoma cell lines were established which secreted monoclonal antibodies (MAbs) against this cytokine. Specific binding of each of the MAbs to recombinant BoIL-4 produced by Escherichia coli, baculovirus, and Trypanosoma brucei was demonstrated in an indirect ELISA and/or in Western blotting. These MAbs recognise the same antigenic region localised in the first 47 amino acids of the mature protein. None of them was able to neutralise the biological activity of the BoIL-4 under the conditions tested but one allowed the detection of BoIL-4 by flow cytometry.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bovinos
/
Interleucina-4
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Ano de publicação:
1998
Tipo de documento:
Article