The CGRP receptor can couple via pertussis toxin sensitive and insensitive G proteins.
FEBS Lett
; 441(1): 6-10, 1998 Dec 11.
Article
em En
| MEDLINE
| ID: mdl-9877154
ABSTRACT
Swiss 3T3 cell lines were constructed co-expressing receptor activity modifying protein (RAMP) 1 with the calcitonin receptor-like receptor (CRLR), and showed 125I-calcitonin-gene-related peptide (CGRP) 1 binding indicative of a type I CGRP receptor. Application of CGRP1 led to an increase in cAMP, which in 2/5 cell lines was augmented following pertussis toxin (PTX) pre-treatment. In Xenopus oocytes, expression of RAMP1, which potentiates an endogenous CGRP receptor, led to constitutive activation of co-expressed GIRK potassium channels. This potassium current was increased following CGRP application or co-expression of CRLR, but decreased by PTX or co-expression of transducin. We conclude that the CGRP receptor can signal to both PTX sensitive and insensitive G proteins.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Peptídeo Relacionado com o Gene de Calcitonina
/
Receptores da Calcitonina
/
Proteínas de Ligação ao GTP
/
Canais de Potássio Corretores do Fluxo de Internalização
/
Proteínas de Membrana
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1998
Tipo de documento:
Article