Determination of the dissociation constants of ropinirole and some impurities and their quantification using capillary zone electrophoresis.
J Chromatogr B Biomed Sci Appl
; 720(1-2): 197-204, 1998 Dec 11.
Article
em En
| MEDLINE
| ID: mdl-9892082
ABSTRACT
Ropinirole, 4-[2-(dipropylamino)ethyl]-1,3-dihydro-2H-indol-2-one, is a potent anti-Parkinson's disease drug developed by SmithKline Beecham Pharmaceuticals. Capillary zone electrophoresis (CZE) was used for the determination of the dissociation constants of ropinirole and five structurally related impurities, potentially formed during its synthesis and for separation and quantification of these substances. The dissociation constants obtained from the CZE measurements were confirmed by UV spectrophotometry for some of the test compounds, obtaining a good agreement between the values. Careful optimization of the running buffer composition permitted base-line resolution of the six compounds in a borate buffer containing acetonitrile and magnesium sulfate (a 100 mM borate buffer containing 30 mM MgSO4 and 20 vol.% of acetonitrile). It was shown that CZE can determine the level of these impurities, down to a level of 0.05% of the main component within 15 min.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eletroforese Capilar
/
Indóis
/
Antiparkinsonianos
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Ano de publicação:
1998
Tipo de documento:
Article