Influence of racial crossing on testicular structure and spermatogenesis in sheep Santa Inês and crossbred Santa Inês and Dorper

The study aimed at evaluating the testicular structure and spermatogenesis of Santa Inês and Santa Inês and Dorper crossbred sheep. Sixteen testicles of the animals under study were used, in order to evaluate the volumetric proportion, the diameter of the tubules, the height of seminiferous epithelium, the frequency of stages of the seminiferous epithelium cycle and the spermatogenic yield. The data were submitted to Student-Newman-Keulstestat 5% significance. The tubular diameter was 173.12 ± 29.09 μm and 185.71 ± 29.7 μm, and the height of the seminiferous epithelium was 52.29 ± 9.98μm and 56.68 ± 11.25 μ m, for the crossbred and the Santa Inês, respectively. There was no difference between the testicular compartments and the frequency of the stages of the seminiferous epithelium cycle between the groups. Santa Inês sheep presented a number of 15.36 ± 4.49 spermatocytes in the pre-leptotene/leptotene stage and 27.42 ± 6.65 spermatocytes in the pachytene, whereas the crossbred presented 13.18 ± 5.19 and 23.48 ± 7.80, respectively. The crossbred showed higher meiotic yield (3.98 ± 1.28) and spermatogenesis yield (3.71 ± 1.02). It is concluded there are differences in testicular morphology and spermatogenesis between Santa Inês sheep and crossbred Dorper/Santa Inês, indicating that the crossbreeding between the Santa Inês and Dorper animals allows a gain in the reproductive potential.

Influence of racial crossing on testicular structure and spermatogenesis in sheep Santa Inês and crossbred Santa Inês and Dorper

The study aimed at evaluating the testicular structure and spermatogenesis of Santa Inês and Santa Inês and Dorper crossbred sheep. Sixteen testicles of the animals under study were used, in order to evaluate the volumetric proportion, the diameter of the tubules, the height of seminiferous epithelium, the frequency of stages of the seminiferous epithelium cycle and the spermatogenic yield. The data were submitted to Student-Newman-Keulstestat 5% significance. The tubular diameter was 173.12 ± 29.09 μm and 185.71 ± 29.7 μm, and the height of the seminiferous epithelium was 52.29 ± 9.98μm and 56.68 ± 11.25 μ m, for the crossbred and the Santa Inês, respectively. There was no difference between the testicular compartments and the frequency of the stages of the seminiferous epithelium cycle between the groups. Santa Inês sheep presented a number of 15.36 ± 4.49 spermatocytes in the pre-leptotene/leptotene stage and 27.42 ± 6.65 spermatocytes in the pachytene, whereas the crossbred presented 13.18 ± 5.19 and 23.48 ± 7.80, respectively. The crossbred showed higher meiotic yield (3.98 ± 1.28) and spermatogenesis yield (3.71 ± 1.02). It is concluded there are differences in testicular morphology and spermatogenesis between Santa Inês sheep and crossbred Dorper/Santa Inês, indicating that the crossbreeding between the Santa Inês and Dorper animals allows a gain in the reproductive potential.(AU)

Contagem celular e rendimento da espermatogênese em ovinos Santa Inês e mestiços Santa Inês e Dorper / Cell count and efficiency of spermatogenesis in ovine Santa Ines and mongrel Santa Inês and Dorper

With the objective to evaluate the efficiency spermatogenesis between sheep breeds, sixteen testicles,eight sheep, four belonging to the race Santa Ines and four of mongrel of Santa Inês and Dorper. After thecastration of animals, we obtained the gonadosomatic index. Generally the average diameter, relative frequencyof the stages in the life cycle of the seminiferous epithelium, yield of spermatogenesis, yield, meioticspermatogenic yield and efficiency of Sertoli cells. There were no significant differences between the races onthe phases of the cycle of the seminiferous epithelium, number of spermatids rounded, Sertoli cells, mitotic rateand effectiveness of Sertoli cells. However, the animals mongrel were higher as the population ofspermatogonia, primary spermatocytes in paquíteno and in pre-leptóteno/leptóteno, yield and meioticspermatogenic yield line. The study showed that animals mongrel of sheep breeds Santa Inês and Dorperpresented higher reproductive potential when compared with pure lambs of Santa Ines.(AU)

Biometria escroto-testicular de carneiros da raça Santa Inês e mestiços (Dorper + Santa Inês) criados sob sistema intensivo / Biometry scrotal-testicular of rams race Santa Inês and mestizos (Dorper + Santa Inês) crated under intensive system

The objective of this study was to evaluate comparatively the scrotum, scrotal circumference of thebreed rams Santa Ines and mestizos (Dorper + Santa Inês) created under intensive system. Used four rams eachrace healthy, aged between 18 and 24 months and scores ranging from 3 to 3.5. There were two collections ofscrotum-testis to determine the characteristics for each group, seven days apart. The data were submitted toanalysis of variance (ANOVA) for a randomized block design. The variables analyzed were submitted to Tukeytest at 5% probability, to compare the scrotum-testicular parameters between the races. Based on the resultsobtained, it is concluded that the scrotum-testicular parameters animal Santa Inês and mestizos (Dorper + SantaInês) created under confinement do not differ significantly (P <0.05).(AU)

Histometria dos túbulos seminíferos em testículos de ovinos Santa Inês e mestiços Santa Inês e Dorper / Histometry of seminiferous tubules in testes of ovine Santa Ines and mongrel Santa Inês and Dorper

The morphometric measurements of the testicles is intended to measure the dimensions of constituenttestis parenchyma quantitatively evaluating spermatogenesis. This study aimed to analyze the spermatogenesisof sheep Santa Ines and crossbred Santa Ines and Dorper watching the racial crossing alter testis function. Weused eight sheep, four Santa Ines and four crossbreed of Santa Ines/Dorper. After castration the animals gavethe Gonadosomatic index. Gauged to volume ratio of testis compartments using a reticle. The constituents of thetubular casing were evaluated: Lamina propria, seminiferous epithelium of the lumen and interstitialcompartment constituents: Leydig cells, blood vessels and connective tissue. Only the tubular diameter andheight of the seminiferous epithelium showed significant differences, being higher for sheep Santa Ines. It wasconcluded that there was little difference in testicular morphometry of animals studied and it was found that theracial cross does not induce significant changes in parameters between groups. (AU)

Biometria escroto-testicular de carneiros da raça Santa Inês e mestiços (Dorper + Santa Inês) criados sob sistema intensivo / Biometry scrotal-testicular of rams race Santa Inês and mestizos (Dorper + Santa Inês) crated under intensive system

The objective of this study was to evaluate comparatively the scrotum, scrotal circumference of thebreed rams Santa Ines and mestizos (Dorper + Santa Inês) created under intensive system. Used four rams eachrace healthy, aged between 18 and 24 months and scores ranging from 3 to 3.5. There were two collections ofscrotum-testis to determine the characteristics for each group, seven days apart. The data were submitted toanalysis of variance (ANOVA) for a randomized block design. The variables analyzed were submitted to Tukeytest at 5% probability, to compare the scrotum-testicular parameters between the races. Based on the resultsobtained, it is concluded that the scrotum-testicular parameters animal Santa Inês and mestizos (Dorper + SantaInês) created under confinement do not differ significantly (P <0.05).

Contagem celular e rendimento da espermatogênese em ovinos Santa Inês e mestiços Santa Inês e Dorper / Cell count and efficiency of spermatogenesis in ovine Santa Ines and mongrel Santa Inês and Dorper

With the objective to evaluate the efficiency spermatogenesis between sheep breeds, sixteen testicles,eight sheep, four belonging to the race Santa Ines and four of mongrel of Santa Inês and Dorper. After thecastration of animals, we obtained the gonadosomatic index. Generally the average diameter, relative frequencyof the stages in the life cycle of the seminiferous epithelium, yield of spermatogenesis, yield, meioticspermatogenic yield and efficiency of Sertoli cells. There were no significant differences between the races onthe phases of the cycle of the seminiferous epithelium, number of spermatids rounded, Sertoli cells, mitotic rateand effectiveness of Sertoli cells. However, the animals mongrel were higher as the population ofspermatogonia, primary spermatocytes in paquíteno and in pre-leptóteno/leptóteno, yield and meioticspermatogenic yield line. The study showed that animals mongrel of sheep breeds Santa Inês and Dorperpresented higher reproductive potential when compared with pure lambs of Santa Ines.

Histometria dos túbulos seminíferos em testículos de ovinos Santa Inês e mestiços Santa Inês e Dorper / Histometry of seminiferous tubules in testes of ovine Santa Ines and mongrel Santa Inês and Dorper

The morphometric measurements of the testicles is intended to measure the dimensions of constituenttestis parenchyma quantitatively evaluating spermatogenesis. This study aimed to analyze the spermatogenesisof sheep Santa Ines and crossbred Santa Ines and Dorper watching the racial crossing alter testis function. Weused eight sheep, four Santa Ines and four crossbreed of Santa Ines/Dorper. After castration the animals gavethe Gonadosomatic index. Gauged to volume ratio of testis compartments using a reticle. The constituents of thetubular casing were evaluated: Lamina propria, seminiferous epithelium of the lumen and interstitialcompartment constituents: Leydig cells, blood vessels and connective tissue. Only the tubular diameter andheight of the seminiferous epithelium showed significant differences, being higher for sheep Santa Ines. It wasconcluded that there was little difference in testicular morphometry of animals studied and it was found that theracial cross does not induce significant changes in parameters between groups.