Resumo
The need for scientific development in goat became larger in order to subsidize farmers withbiotechnology to animal breeding. The advent of frozen semen increased by male progeny, possiblemanipulation and genetic material storage. It seeks to diluents free of animal substances, to ensure food safety inbiological processes. The objective of this research was seminal diluent develop using Mauritia flexuoxa extractas an additive. Obtaining the extract was from dehydrated raw materials, semen collection was performed withartificial vagina and in vitro spermatic evaluation through full force and motility. The extract prepared in TRISdiluent is included at different concentrations. Semen was diluted and evaluated the heat resistance at 37°C. Weevaluated the membrane integrity after 5, 10, 15, 30, 60 and 120 minutes of incubation at 37°C. It wasconcluded that significant differences were observed using Mauritia flexuosa extract.(AU)
Assuntos
Animais , Masculino , Análise do Sêmen/veterinária , Magnoliopsida/efeitos adversos , Magnoliopsida/química , RuminantesResumo
The need for scientific development in goat became larger in order to subsidize farmers withbiotechnology to animal breeding. The advent of frozen semen increased by male progeny, possiblemanipulation and genetic material storage. It seeks to diluents free of animal substances, to ensure food safety inbiological processes. The objective of this research was seminal diluent develop using Mauritia flexuoxa extractas an additive. Obtaining the extract was from dehydrated raw materials, semen collection was performed withartificial vagina and in vitro spermatic evaluation through full force and motility. The extract prepared in TRISdiluent is included at different concentrations. Semen was diluted and evaluated the heat resistance at 37°C. Weevaluated the membrane integrity after 5, 10, 15, 30, 60 and 120 minutes of incubation at 37°C. It wasconcluded that significant differences were observed using Mauritia flexuosa extract.