Reproductive performance of sows inseminated with semen doses stored for up to seven days in long-term extender in a field condition

This study aimed to evaluate the reproductive performance of sows inseminated with semen doses preserved at 15-18 °C for up to seven days in long-term extender (Duragen®). Parity one (PO1) to PO7 sows were randomly assigned to the following groups: AI1-3 (n=190), insemination with semen doses stored between one and three days; and AI5-7 (n=124), insemination with semen doses stored between five and seven days. Sows were submitted to estrus detection twice a day. Post-cervical insemination according to weaning-to-estrus interval was performed. The farrowing rate (FR) did not differ between the groups (AI1-3=83.2%; AI5-7=82.2%; p>0.05) nor did the total number of piglets born (TPB; AI1-3=14.2±0.3; AI5-7=14.5±0.3; p>0.05). Considering the semen dose most likely responsible for fertilization according to its storage time (1, 2-3, 5, and 6-7 days), the FR (72.7%, 87.8%, 85.7%, and 79%, respectively) and TPB (14.4, 14.0, 14.9, and 13.5, respectively) were similar among the groups (p>0.05). In conclusion, the use of semen doses extended with long-term extender stored for up to seven days did not impair the reproductive performance of sows. Therefore, it’s using could optimize production efficiency and logistics of semen dose deliveries to sow farms.

Reproductive performance of sows inseminated with semen doses stored for up to seven days in long-term extender in a field condition

This study aimed to evaluate the reproductive performance of sows inseminated with semen doses preserved at 15-18 °C for up to seven days in long-term extender (Duragen®). Parity one (PO1) to PO7 sows were randomly assigned to the following groups: AI1-3 (n=190), insemination with semen doses stored between one and three days; and AI5-7 (n=124), insemination with semen doses stored between five and seven days. Sows were submitted to estrus detection twice a day. Post-cervical insemination according to weaning-to-estrus interval was performed. The farrowing rate (FR) did not differ between the groups (AI1-3=83.2%; AI5-7=82.2%; p>0.05) nor did the total number of piglets born (TPB; AI1-3=14.2±0.3; AI5-7=14.5±0.3; p>0.05). Considering the semen dose most likely responsible for fertilization according to its storage time (1, 2-3, 5, and 6-7 days), the FR (72.7%, 87.8%, 85.7%, and 79%, respectively) and TPB (14.4, 14.0, 14.9, and 13.5, respectively) were similar among the groups (p>0.05). In conclusion, the use of semen doses extended with long-term extender stored for up to seven days did not impair the reproductive performance of sows. Therefore, its using could optimize production efficiency and logistics of semen dose deliveries to sow farms.(AU)

Inseminação artificial intra-uterina em leitoas com sêmen criopreservado com dimetilacetamida e glicerol

The objective of this study was to evaluate the use of dimethylacetamide (DMA) and glycerol in boar semen cryopreservation on the conception and fertility rates.Sixty pre-pubertal gilts were synchronized and inseminated with semen including either glycerol 3% or DMA 5% as cryoprotectants (n=30 for both groups). Artificial insemination was conducted with the intrauterine method using doses with 1 x 10(9) viable spermatozoa. The gilts were slaughtered 36-40h after the insemination and the hemorrhagic bodies (CH) in the ovaries were counted. The oviducts were washed, so oocytes and embryos could be recovered to determine the conception and fertility rates. The average CH obtained with glycerol 3% did not differ (P>0.05) from those observed with DMA (10.4 x 10.2, respectively). Recovery rates also did not differ (P>0.05) between groups (68.9% with glycerol and 66.9% with DMA). Conception (73.3 x 76.6%) and fertility rate (48.6 x 59.4%) were also similar (P>0.05) for glycerol and DMA, respectively. Those results indicate that DMA 5% can replace glycerol 3% in the composition of extenders for frozen boar semen.

Este estudo teve por objetivo avaliar o uso da dimetilacetamida (DMA) e de glicerol na criopreservação de sêmen suíno sobre as taxas de concepção e fertilização in vivo, utilizando o método de inseminação artificial pós-cervical. Foram sincronizadas 60 leitoas pré-púberes e inseminadas com o uso de sêmen congelado com glicerol 3% (30 fêmeas) e DMA 5% (30 fêmeas). O método de inseminação utilizado foi o pós-cervical, com concentração de 1 x 10(9) espermatozóides vivos por dose. Após 36 a 40h da inseminação, as fêmeas foram abatidas, sendo realizada a contagem de corpos hemorrágicos (CH) nos ovários. Foi realizada a lavagem dos ovidutos das fêmeas, verificando o número de estruturas recuperadas (oócitos e embriões), calculando-se as taxas de concepção e fertilização. A média de CH nas fêmeas do grupo glicerol 3% não diferiu (P>0,05) daquelas do grupo DMA 5% (10,4 x 10,2, respectivamente). Não houve diferença (P>0,05) nas taxas de recuperação de estruturas entre os grupos glicerol 3% (68,9%) e DMA 5% (66,9%). Os resultados obtidos nos grupos glicerol 3% e DMA 5% para as taxas de concepção (73,3 x 76,6%) e fertilização (48,6 x 59,4%) não apresentaram diferença (P>0,05). Conclui-se que não há diferenças nas taxas de concepção e fertilização in vivo utilizando-se sêmen congelado com o uso de dimetilacetamida ou de glicerol.

Inseminação artificial intra-uterina em leitoas com sêmen criopreservado com dimetilacetamida e glicerol

The objective of this study was to evaluate the use of dimethylacetamide (DMA) and glycerol in boar semen cryopreservation on the conception and fertility rates.Sixty pre-pubertal gilts were synchronized and inseminated with semen including either glycerol 3% or DMA 5% as cryoprotectants (n=30 for both groups). Artificial insemination was conducted with the intrauterine method using doses with 1 x 10(9) viable spermatozoa. The gilts were slaughtered 36-40h after the insemination and the hemorrhagic bodies (CH) in the ovaries were counted. The oviducts were washed, so oocytes and embryos could be recovered to determine the conception and fertility rates. The average CH obtained with glycerol 3% did not differ (P>0.05) from those observed with DMA (10.4 x 10.2, respectively). Recovery rates also did not differ (P>0.05) between groups (68.9% with glycerol and 66.9% with DMA). Conception (73.3 x 76.6%) and fertility rate (48.6 x 59.4%) were also similar (P>0.05) for glycerol and DMA, respectively. Those results indicate that DMA 5% can replace glycerol 3% in the composition of extenders for frozen boar semen.

Este estudo teve por objetivo avaliar o uso da dimetilacetamida (DMA) e de glicerol na criopreservação de sêmen suíno sobre as taxas de concepção e fertilização in vivo, utilizando o método de inseminação artificial pós-cervical. Foram sincronizadas 60 leitoas pré-púberes e inseminadas com o uso de sêmen congelado com glicerol 3% (30 fêmeas) e DMA 5% (30 fêmeas). O método de inseminação utilizado foi o pós-cervical, com concentração de 1 x 10(9) espermatozóides vivos por dose. Após 36 a 40h da inseminação, as fêmeas foram abatidas, sendo realizada a contagem de corpos hemorrágicos (CH) nos ovários. Foi realizada a lavagem dos ovidutos das fêmeas, verificando o número de estruturas recuperadas (oócitos e embriões), calculando-se as taxas de concepção e fertilização. A média de CH nas fêmeas do grupo glicerol 3% não diferiu (P>0,05) daquelas do grupo DMA 5% (10,4 x 10,2, respectivamente). Não houve diferença (P>0,05) nas taxas de recuperação de estruturas entre os grupos glicerol 3% (68,9%) e DMA 5% (66,9%). Os resultados obtidos nos grupos glicerol 3% e DMA 5% para as taxas de concepção (73,3 x 76,6%) e fertilização (48,6 x 59,4%) não apresentaram diferença (P>0,05). Conclui-se que não há diferenças nas taxas de concepção e fertilização in vivo utilizando-se sêmen congelado com o uso de dimetilacetamida ou de glicerol.