Porphyromonas gingivalis in the Oral Cavity of English Bulldog Newborn Puppies

Background: Periodontal disease (PD) is the most common disease of the oral cavity in cats and dogs, and it affects up to 80% of these animals. PD begins with the accumulation of bacteria on the surface of the teeth, and it poses a risk for the health of pets. Research on PD in dogs has focused on the identification and characterization of bacterial communities present in the oral cavity. Porphyromonas gingivalis is highly prevalent in the oral cavity. Therefore, the aim of this study was to detect P. gingivalis before and after dental eruption in 15 English bulldog newborn puppies, hoping to contribute to early guidance of oral hygiene management and prevent future PD.Materials, Methods & Results: Fifteen English bulldog newborn puppies were used in this study. Two groups (G1 and G2) were formed with eight and seven puppies, respectively. Oral swab samples were taken from the maxillary incisor region of animals from G1 and G2 10 days after birth (T10). At this moment, the clinical evaluation of the oral cavity showed healthy gums with a thin, shiny, pinkish, and firm margin, without any odor or granular appearance, and with no tooth eruption. On postnatal day 25 (T25), a subgingival sample was collected with a Gracey curette from the maxillary incisors; the oral cavity examination revealed healthy gums and presence of gingival sulcus. Bilateral subgingival samples were als

Porphyromonas gingivalis in the Oral Cavity of English Bulldog Newborn Puppies

Background: Periodontal disease (PD) is the most common disease of the oral cavity in cats and dogs, and it affects up to 80% of these animals. PD begins with the accumulation of bacteria on the surface of the teeth, and it poses a risk for the health of pets. Research on PD in dogs has focused on the identification and characterization of bacterial communities present in the oral cavity. Porphyromonas gingivalis is highly prevalent in the oral cavity. Therefore, the aim of this study was to detect P. gingivalis before and after dental eruption in 15 English bulldog newborn puppies, hoping to contribute to early guidance of oral hygiene management and prevent future PD.Materials, Methods & Results: Fifteen English bulldog newborn puppies were used in this study. Two groups (G1 and G2) were formed with eight and seven puppies, respectively. Oral swab samples were taken from the maxillary incisor region of animals from G1 and G2 10 days after birth (T10). At this moment, the clinical evaluation of the oral cavity showed healthy gums with a thin, shiny, pinkish, and firm margin, without any odor or granular appearance, and with no tooth eruption. On postnatal day 25 (T25), a subgingival sample was collected with a Gracey curette from the maxillary incisors; the oral cavity examination revealed healthy gums and presence of gingival sulcus. Bilateral subgingival samples were als

Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes) / Diagnóstico molecular da leishmaniose visceral canina através da técnica de sonda de nanopartículas de ouro (AuNPprobes)

The aim of this study was to comparatively evaluate the diagnostic methods of Canine Visceral Leishmaniasis (CVL), using the indirect immunofluorescence assay (IFA), the probe Gold Nanoparticles (AuNPprobes) and PCR in liver samples. We analyzed 25 samples of animals positive by IFA and 10 samples from animals negative to PCR. The probe AUNP used was based on specific sequence for Leishmania infantum kDNA. The results showed that when compared to the IFA sorology the AuNPprobe showed a sensitivity and specificity of 56% and 80%, respectively. When compared to PCR method, the AuNPprobe had a sensitivity of 68% and specificity of 81%. The AuNPprobe can be an alternative to traditional diagnostic methods (PCR and IFA) due to its ease of implementation, immediate result, low cost and little need for laboratory infrastructure.

O objetivo da presente pesquisa foi avaliar comparativamente os métodos diagnósticos da Leishmaniose Visceral Canina (LVC), utilizando-se a reação de imunofluorescência indireta (RIFI), a sonda de Nanopartículas de Ouro (AuNPprobes) e a PCR em amostras de fígado. Foram analisadas 25 amostras de animais positivos pela RIFI e 10 amostras de animais negativos à PCR. A AuNPprobes utilizada baseou-se na sequência de kDNA específico para Leishmania infantum. Os resultados demonstraram que ao ser comparada com a RIFI a AuNPprobes apresentou sensibilidade e especificidade de 56% e 80%, respectivamente. Já ao ser comparada à PCR, a AuNPprobes apresentou sensibilidade de 68% e especificidade de 81%. A AuNPprobes pode ser uma alternativa aos métodos de diagnósticos tradicionais (PCR e RIFI), devido a sua facilidade de execução, rapidez, baixo custo e necessidade de pouca infraestrutura laboratorial.

Porphyromonas gingivalis in the Oral Cavity of English Bulldog Newborn Puppies

Background: Periodontal disease (PD) is the most common disease of the oral cavity in cats and dogs, and it affects up to 80% of these animals. PD begins with the accumulation of bacteria on the surface of the teeth, and it poses a risk for the health of pets. Research on PD in dogs has focused on the identification and characterization of bacterial communities present in the oral cavity. Porphyromonas gingivalis is highly prevalent in the oral cavity. Therefore, the aim of this study was to detect P. gingivalis before and after dental eruption in 15 English bulldog newborn puppies, hoping to contribute to early guidance of oral hygiene management and prevent future PD.Materials, Methods & Results: Fifteen English bulldog newborn puppies were used in this study. Two groups (G1 and G2) were formed with eight and seven puppies, respectively. Oral swab samples were taken from the maxillary incisor region of animals from G1 and G2 10 days after birth (T10). At this moment, the clinical evaluation of the oral cavity showed healthy gums with a thin, shiny, pinkish, and firm margin, without any odor or granular appearance, and with no tooth eruption. On postnatal day 25 (T25), a subgingival sample was collected with a Gracey curette from the maxillary incisors; the oral cavity examination revealed healthy gums and presence of gingival sulcus. Bilateral subgingival samples were als

Porphyromonas gingivalis in the Oral Cavity of English Bulldog Newborn Puppies

Background: Periodontal disease (PD) is the most common disease of the oral cavity in cats and dogs, and it affects up to 80% of these animals. PD begins with the accumulation of bacteria on the surface of the teeth, and it poses a risk for the health of pets. Research on PD in dogs has focused on the identification and characterization of bacterial communities present in the oral cavity. Porphyromonas gingivalis is highly prevalent in the oral cavity. Therefore, the aim of this study was to detect P. gingivalis before and after dental eruption in 15 English bulldog newborn puppies, hoping to contribute to early guidance of oral hygiene management and prevent future PD.Materials, Methods & Results: Fifteen English bulldog newborn puppies were used in this study. Two groups (G1 and G2) were formed with eight and seven puppies, respectively. Oral swab samples were taken from the maxillary incisor region of animals from G1 and G2 10 days after birth (T10). At this moment, the clinical evaluation of the oral cavity showed healthy gums with a thin, shiny, pinkish, and firm margin, without any odor or granular appearance, and with no tooth eruption. On postnatal day 25 (T25), a subgingival sample was collected with a Gracey curette from the maxillary incisors; the oral cavity examination revealed healthy gums and presence of gingival sulcus. Bilateral subgingival samples were als

Porphyromonas gingivalis in the Oral Cavity of English Bulldog Newborn Puppies

Background: Periodontal disease (PD) is the most common disease of the oral cavity in cats and dogs, and it affects up to 80% of these animals. PD begins with the accumulation of bacteria on the surface of the teeth, and it poses a risk for the health of pets. Research on PD in dogs has focused on the identification and characterization of bacterial communities present in the oral cavity. Porphyromonas gingivalis is highly prevalent in the oral cavity. Therefore, the aim of this study was to detect P. gingivalis before and after dental eruption in 15 English bulldog newborn puppies, hoping to contribute to early guidance of oral hygiene management and prevent future PD.Materials, Methods & Results: Fifteen English bulldog newborn puppies were used in this study. Two groups (G1 and G2) were formed with eight and seven puppies, respectively. Oral swab samples were taken from the maxillary incisor region of animals from G1 and G2 10 days after birth (T10). At this moment, the clinical evaluation of the oral cavity showed healthy gums with a thin, shiny, pinkish, and firm margin, without any odor or granular appearance, and with no tooth eruption. On postnatal day 25 (T25), a subgingival sample was collected with a Gracey curette from the maxillary incisors; the oral cavity examination revealed healthy gums and presence of gingival sulcus. Bilateral subgingival samples were als

Porphyromonas gingivalis in the Oral Cavity of English Bulldog Newborn Puppies

Background: Periodontal disease (PD) is the most common disease of the oral cavity in cats and dogs, and it affects up to 80% of these animals. PD begins with the accumulation of bacteria on the surface of the teeth, and it poses a risk for the health of pets. Research on PD in dogs has focused on the identification and characterization of bacterial communities present in the oral cavity. Porphyromonas gingivalis is highly prevalent in the oral cavity. Therefore, the aim of this study was to detect P. gingivalis before and after dental eruption in 15 English bulldog newborn puppies, hoping to contribute to early guidance of oral hygiene management and prevent future PD.Materials, Methods & Results: Fifteen English bulldog newborn puppies were used in this study. Two groups (G1 and G2) were formed with eight and seven puppies, respectively. Oral swab samples were taken from the maxillary incisor region of animals from G1 and G2 10 days after birth (T10). At this moment, the clinical evaluation of the oral cavity showed healthy gums with a thin, shiny, pinkish, and firm margin, without any odor or granular appearance, and with no tooth eruption. On postnatal day 25 (T25), a subgingival sample was collected with a Gracey curette from the maxillary incisors; the oral cavity examination revealed healthy gums and presence of gingival sulcus. Bilateral subgingival samples were als

Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes) / Diagnóstico molecular da leishmaniose visceral canina através da técnica de sonda de nanopartículas de ouro (AuNPprobes)

The aim of this study was to comparatively evaluate the diagnostic methods of Canine Visceral Leishmaniasis (CVL), using the indirect immunofluorescence assay (IFA), the probe Gold Nanoparticles (AuNPprobes) and PCR in liver samples. We analyzed 25 samples of animals positive by IFA and 10 samples from animals negative to PCR. The probe AUNP used was based on specific sequence for Leishmania infantum kDNA. The results showed that when compared to the IFA sorology the AuNPprobe showed a sensitivity and specificity of 56% and 80%, respectively. When compared to PCR method, the AuNPprobe had a sensitivity of 68% and specificity of 81%. The AuNPprobe can be an alternative to traditional diagnostic methods (PCR and IFA) due to its ease of implementation, immediate result, low cost and little need for laboratory infrastructure.

O objetivo da presente pesquisa foi avaliar comparativamente os métodos diagnósticos da Leishmaniose Visceral Canina (LVC), utilizando-se a reação de imunofluorescência indireta (RIFI), a sonda de Nanopartículas de Ouro (AuNPprobes) e a PCR em amostras de fígado. Foram analisadas 25 amostras de animais positivos pela RIFI e 10 amostras de animais negativos à PCR. A AuNPprobes utilizada baseou-se na sequência de kDNA específico para Leishmania infantum. Os resultados demonstraram que ao ser comparada com a RIFI a AuNPprobes apresentou sensibilidade e especificidade de 56% e 80%, respectivamente. Já ao ser comparada à PCR, a AuNPprobes apresentou sensibilidade de 68% e especificidade de 81%. A AuNPprobes pode ser uma alternativa aos métodos de diagnósticos tradicionais (PCR e RIFI), devido a sua facilidade de execução, rapidez, baixo custo e necessidade de pouca infraestrutura laboratorial.