Resumo
Dye-test and indirect antiglobulin fluorescent test for toxoplasma antibodies were compared in 1,000 human sera. Both techniques were described in detail, with special emphasis on preparation of antigens and conjugates for the fluorescent test. The tests were studied as to reproductibility of results, and a close agreement was found for sucessive titrations of same sera. Dye-test and fluorescent test compared well, with an almost total coincidence of results as to reactivity of sera. Titers were the same or differed by only one dilution in 97.7% of reactive sera and by only two dilutions in the remaining 2,3%. A small number of cases was seen with unexpected temporary divergences, as they reacted only in the dye-test when first examined. However, when tested again after being frozen for a few hours or days, they furnished entirely negative results. A cytoplasm-modifying factor different from that responsible for immune-reactions was suggested to occur in such sera.
Compararam-se as reações de imunofluorescência indireta e de SABIN-FELDMAN para a toxoplasmose, em 1000 soros humanos. Encontrou-se estreita concordância quanto a reatividade ou não reatividade dos soros. Quanto a títulos, em 97,7% dos 775 soros reagentes verificou-se concordância ou diferenças de somente uma diluição. Nos demais 2,3% de soros reagentes, estas diferenças não excederam de duas diluições. Em raros soros, entretanto, observaram-se divergências entre os resultados, negativos na reação de imunofluorescência e reagentes na de SABIN-FEDLMAN. Porém tal reatividade apresentou-se instável, tendendo a desaparecer rapidamente desses soros, embora mantidos congelados a -20C, em contraste com a estabilidade de títulos observada habitualmente nos soros reagentes. Sugere-se a presença de fator modificador de citoplasma, de natureza inespecífica, para os soros de reatividade instável, diverso do fator específico presente no comum dos soros reagentes.
Resumo
Dye-test and indirect antiglobulin fluorescent test for toxoplasma antibodies were compared in 1,000 human sera. Both techniques were described in detail, with special emphasis on preparation of antigens and conjugates for the fluorescent test. The tests were studied as to reproductibility of results, and a close agreement was found for sucessive titrations of same sera. Dye-test and fluorescent test compared well, with an almost total coincidence of results as to reactivity of sera. Titers were the same or differed by only one dilution in 97.7% of reactive sera and by only two dilutions in the remaining 2,3%. A small number of cases was seen with unexpected temporary divergences, as they reacted only in the dye-test when first examined. However, when tested again after being frozen for a few hours or days, they furnished entirely negative results. A cytoplasm-modifying factor different from that responsible for immune-reactions was suggested to occur in such sera.
Compararam-se as reações de imunofluorescência indireta e de SABIN-FELDMAN para a toxoplasmose, em 1000 soros humanos. Encontrou-se estreita concordância quanto a reatividade ou não reatividade dos soros. Quanto a títulos, em 97,7% dos 775 soros reagentes verificou-se concordância ou diferenças de somente uma diluição. Nos demais 2,3% de soros reagentes, estas diferenças não excederam de duas diluições. Em raros soros, entretanto, observaram-se divergências entre os resultados, negativos na reação de imunofluorescência e reagentes na de SABIN-FEDLMAN. Porém tal reatividade apresentou-se instável, tendendo a desaparecer rapidamente desses soros, embora mantidos congelados a -20C, em contraste com a estabilidade de títulos observada habitualmente nos soros reagentes. Sugere-se a presença de fator modificador de citoplasma, de natureza inespecífica, para os soros de reatividade instável, diverso do fator específico presente no comum dos soros reagentes.
Resumo
Summary The authors present the first six possible autoctonus serologically positive cases of Chagas disease in farmers from municipality of Barcelos, in the State of Amazonas, Brazil. This region has many "piaçaba" palm trees (Leopoldinia piassaba) which harbor numerous triatomes in nature.
Resumo
Summary The authors present the first six possible autoctonus serologically positive cases of Chagas disease in farmers from municipality of Barcelos, in the State of Amazonas, Brazil. This region has many "piaçaba" palm trees (Leopoldinia piassaba) which harbor numerous triatomes in nature.