Use of the software Seed Vigor Imaging System (SVIS®) for assessing vigor of carrot seeds

Seed vigor has traditionally been evaluated by physiological, biochemical and stress tolerance tests. More recently, with the use of computerized image analysis, objective information has become accessible in a relatively short period of time, with less human interference. The aim of this study was to verify the efficiency of computerized seedling image analysis by Seed Vigor Imaging System (SVIS®) to detect differences in vigor between carrot (Daucus carota L.) seed lots as compared to those provided by traditional vigor tests. Seeds from seven lots from the Brasilia cultivar were subjected to a germination test, first count of germination, speed of germination, accelerated aging with saline solution and seedling emergence; furthermore, a vigor index, growth index and uniformity index were determined by the Seed Vigor Imaging System (SVIS®) during four evaluation periods. The results obtained by the computerized seedling analysis (vigor index and growth index) show that SVIS® is efficient in assessing carrot seed vigor.

Use of the software Seed Vigor Imaging System (SVIS®) for assessing vigor of carrot seeds

Seed vigor has traditionally been evaluated by physiological, biochemical and stress tolerance tests. More recently, with the use of computerized image analysis, objective information has become accessible in a relatively short period of time, with less human interference. The aim of this study was to verify the efficiency of computerized seedling image analysis by Seed Vigor Imaging System (SVIS®) to detect differences in vigor between carrot (Daucus carota L.) seed lots as compared to those provided by traditional vigor tests. Seeds from seven lots from the Brasilia cultivar were subjected to a germination test, first count of germination, speed of germination, accelerated aging with saline solution and seedling emergence; furthermore, a vigor index, growth index and uniformity index were determined by the Seed Vigor Imaging System (SVIS®) during four evaluation periods. The results obtained by the computerized seedling analysis (vigor index and growth index) show that SVIS® is efficient in assessing carrot seed vigor.(AU)

Avaliação do vigor de sementes de girassol por meio de análise de imagens de plântulas / Sunflower seed vigor evaluation by seedling image analyze

O trabalho teve como objetivo avaliar a eficiência do sistema computadorizado de análise de imagens de plântulas Seed Vigor Imaging System (SVIS(r)), em comparação com os outros testes recomendados para determinação do vigor de sementes de girassol. Foram utilizados dez lotes de sementes, cultivar Catissol 01, os quais foram avaliados quanto ao potencial fisiológico por meio dos testes de germinação, envelhecimento acelerado, emergência de plântulas em areia e em campo e análise de imagens de plântulas com o sistema SVIS(r), aos 0 e 4 meses de armazenamento. O delineamento experimental utilizado foi inteiramente casualizado para os testes realizados em laboratório e blocos ao acaso para o teste de emergência de plântulas em campo; em ambos os casos, foram utilizadas 4 repetições. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Scott Knott a 5% de probabilidade. Verificou-se que a análise computadorizada de imagens de plântulas com SVIS(r) é eficiente para avaliação do vigor de sementes de girassol.(AU)

This study aimed to evaluate the efficiency of computerized image analysis of seedling Seed Vigor Imaging System (SVIS(r)) compared with the other recommended tests to determine vigor in sunflower seeds. It was used ten seed lots of cultivar Catissol 01.The physiological potential was assessed using tests of germination, accelerated aging, seedling emergence and seedling image analyze with SVIS(r) at 0 and 4 months after storage. The experimental design was completely randomized for the laboratory tests and randomized blocs to seedling emergence on the field; in both cases it was used four replications. The data were submitted to variance analyze and mean comparisons at Scott Knott test (P 0,01). Seedling image analyze with SVIS(r) is efficient to access sunflower vigor see.(AU)

Tomato seed vigor evaluation during storage by seedling computadorized image analysis / Avaliação do vigor de sementes de tomate durante o armazenamento por meio de análise computadorizada de imagens de plântulas

The aim of this study was to determine the sensitivity of the parameters provided by computerized image analysis to detect tomato seed vigor reduction during storage, compared to tests traditionally used for this purpose. Ten lots of tomato seeds, cultivar Mariana, were stored for 12 months in a controlled environment at 20 ± 1 C and 45-50% relative humidity. The seed moisture content was monitored and physiological potential assessed at 0, 3, 6 and 12 months after storage, with germination, first count, accelerated aging with saturated salt solution, seedling emergence tests and with computerized image analyzes of seedlings by SVIS® system. A completely random design was used in a 4 x 10 factorial scheme (4 evaluations periods X 10 lots) with 4 replications. Data were subjected to analysis of variance and mean comparison by the Scott Knott test at 5% probability. Computer image analysis of tomato seedlings with five days after sowing is able to detect vigor differences between lots and vigor loss during storage.

O objetivo deste trabalho foi verificar a sensibilidade dos parâmetros fornecidos pela análise computadorizada de imagens de plântulas para detectar a redução do vigor de sementes de tomate durante o armazenamento, comparado a testes tradicionalmente utilizados para esta finalidade. Dez lotes de sementes de tomate, cultivar Mariana, foram armazenados por 12 meses em ambiente controlado a 20±1 C e 45-50% de umidade relativa do ar. O teor de água das sementes foi monitorado e o potencial fisiológico avaliado aos 0, 3, 6 e 12 meses após o armazenamento, com os testes de germinação, primeira contagem de germinação, envelhecimento acelerado com solução saturada de sal, emergência de plântulas e com o sistema de análise computadorizada de imagens de plântulas (SVIS). O delineamento experimental utilizado foi inteiramente casualizado em esquema fatorial 4 x 10 (épocas X lotes) com 4 repetições. Os dados foram submetidos à análise de variância e a comparação de médias por meio do teste de Scott Knott a 5% de probabilidade. A análise computadorizada de imagens de plântulas de tomate, cinco dias após a semeadura, permite detectar diferenças de vigor entre lotes e a redução do vigor ao longo do armazenamento.

Tomato seed vigor evaluation during storage by seedling computadorized image analysis / Avaliação do vigor de sementes de tomate durante o armazenamento por meio de análise computadorizada de imagens de plântulas

The aim of this study was to determine the sensitivity of the parameters provided by computerized image analysis to detect tomato seed vigor reduction during storage, compared to tests traditionally used for this purpose. Ten lots of tomato seeds, cultivar Mariana, were stored for 12 months in a controlled environment at 20 ± 1 C and 45-50% relative humidity. The seed moisture content was monitored and physiological potential assessed at 0, 3, 6 and 12 months after storage, with germination, first count, accelerated aging with saturated salt solution, seedling emergence tests and with computerized image analyzes of seedlings by SVIS® system. A completely random design was used in a 4 x 10 factorial scheme (4 evaluations periods X 10 lots) with 4 replications. Data were subjected to analysis of variance and mean comparison by the Scott Knott test at 5% probability. Computer image analysis of tomato seedlings with five days after sowing is able to detect vigor differences between lots and vigor loss during storage.(AU)

O objetivo deste trabalho foi verificar a sensibilidade dos parâmetros fornecidos pela análise computadorizada de imagens de plântulas para detectar a redução do vigor de sementes de tomate durante o armazenamento, comparado a testes tradicionalmente utilizados para esta finalidade. Dez lotes de sementes de tomate, cultivar Mariana, foram armazenados por 12 meses em ambiente controlado a 20±1 C e 45-50% de umidade relativa do ar. O teor de água das sementes foi monitorado e o potencial fisiológico avaliado aos 0, 3, 6 e 12 meses após o armazenamento, com os testes de germinação, primeira contagem de germinação, envelhecimento acelerado com solução saturada de sal, emergência de plântulas e com o sistema de análise computadorizada de imagens de plântulas (SVIS). O delineamento experimental utilizado foi inteiramente casualizado em esquema fatorial 4 x 10 (épocas X lotes) com 4 repetições. Os dados foram submetidos à análise de variância e a comparação de médias por meio do teste de Scott Knott a 5% de probabilidade. A análise computadorizada de imagens de plântulas de tomate, cinco dias após a semeadura, permite detectar diferenças de vigor entre lotes e a redução do vigor ao longo do armazenamento.(AU)