Phytase Superdosing in the Diet of Lightweight Replacement Pullets: Performance, Organ Biometry and Bone Characteristics

The aim of this study was to evaluate the effect of two types of phytases (the 1st produced from Escherichia coli and the 2nd from Aspergillus oryzae) with different dosages (300 and 900 Unit Phytase or FTU) on performance, organ biometry and bone quality of replacement pullets in the period of 8 to 17 weeks of age. A total of 288 Hy-Line White laying hens were used at 8 weeks of age. A completely randomized design was adopted in a 2 x 2 factorial scheme, totaling 4 treatments with 8 replicates of 9 birds each. Performance, biometric variables and bone characteristics were evaluated. There was no significant interaction between the factors for any of the evaluated variables. The conversion and the accumulated consumption were influenced (p 0.05) by the phytase dosages, with the highest results being observed for the consumption obtained with 300 FTU, and the best results for the feed conversion obtained with the dosage of 900 FTU. The relative weight of the liver was influenced by the treatments (p 0.05), presenting higher values with bacterial phytase and a dosage of 900 FTU. The sternum length and tibial deformity were influenced by fungal phytase (p 0.05). It is concluded that the use of 900 FTU superdosing and fungal phytase improves the performance and bone characteristics of light replacement pullets.(AU)

Phytase Superdosing in the Diet of Lightweight Replacement Pullets: Performance, Organ Biometry and Bone Characteristics

The aim of this study was to evaluate the effect of two types of phytases (the 1st produced from Escherichia coli and the 2nd from Aspergillus oryzae) with different dosages (300 and 900 Unit Phytase or FTU) on performance, organ biometry and bone quality of replacement pullets in the period of 8 to 17 weeks of age. A total of 288 Hy-Line White laying hens were used at 8 weeks of age. A completely randomized design was adopted in a 2 x 2 factorial scheme, totaling 4 treatments with 8 replicates of 9 birds each. Performance, biometric variables and bone characteristics were evaluated. There was no significant interaction between the factors for any of the evaluated variables. The conversion and the accumulated consumption were influenced (p 0.05) by the phytase dosages, with the highest results being observed for the consumption obtained with 300 FTU, and the best results for the feed conversion obtained with the dosage of 900 FTU. The relative weight of the liver was influenced by the treatments (p 0.05), presenting higher values with bacterial phytase and a dosage of 900 FTU. The sternum length and tibial deformity were influenced by fungal phytase (p 0.05). It is concluded that the use of 900 FTU superdosing and fungal phytase improves the performance and bone characteristics of light replacement pullets.

Parâmetros qualitativos do sêmen e morfologia comparada da cabeça e peça intermediária do espermatozoide de pirapitinga (Piaractus brachypomus), pacu (Piaractus mesopotamicus) e tambaqui (Colossoma macropomum) / Qualitative semen parameters and comparative morphology of pirapitinga (Piaractus brachypomus), pacu (Piaractus mesopotamicus) and tambaqui (Colossoma macropomum) sperm

O objetivo deste trabalho foi avaliar os parâmetros qualitativos do sêmen e comparar a morfologia da cabeça do espermatozoide entre três espécies de peixes, Piaractus mesopotamicus, Piaractus brachypomus e Colossoma macropomum. As amostras de sêmen foram coletadas de nove animais, três machos de cada espécie, por método de reprodução induzida no mês de novembro de 2011. Os espermatozoides foram submetidos à análise de motilidade e estudo da morfologia em microscopia eletrônica de varredura. As taxas de motilidade das três espécies avaliadas foram acima de 90%, não diferindo entre elas. Porém, a duração de motilidade do sêmen de pacu foi superior as demais espécies. O comprimento da cabeça (CC) dos espermatozóides de pacu, Pirapitinga e Tambaqui foram, respectivamente, 2,43 ± 0,19 μm, 1,94 ± 0,07 μm e 2,44 ± 0,12 μm. A largura da cabeça (LC) foram, respectivamente, 1,45 ± 0,07 μm, 1,76 ± 0,07 μm e 1,56 ± 0,06 μm. O comprimento da peça intermediária foram, respectivamente, 1,00 ± 0,10 μm, 1,54 ± 0,08 μm e 2,07 ± 0,33 μm. Os resultados revelam que os espermatozoides das três espécies diferem entre si na morfologia externa quanto ao tamanho e formato da cabeça, e nas proporções de comprimento da peça intermediária.

The objective of this work was to evaluate qualitative semen parameters and to compare the morphology of the sperm head among three fish species, Piaractus mesopotamicus, Piaractus brachypomus and Colossoma macropomum. The semen samples were collected from nine animals, three males per species, by the induced reproduction method in November 2011. Sperm motility was evaluated and morphology was analyzed by scanning electron microscopy. The motility rates of the three species evaluated were higher than 90%, with no difference between species. However, the duration of sperm motility was longer for pacu semen compared to the other species. The head length was 2.43 ± 0.19, 1.94 ± 0.07 and 2.44 ± 0.12 μm for pacu, pirapitinga and tambaqui sperm, respectively. The head width was 1.45 ± 0.07, 1.76 ± 0.07 and 1.56 ± 0.06 μm, and the length of the intermediate piece was 1.0 ± 0.10, 1.54 ± 0.08 and 2.07 ± 0.33 μm, respectively. The results show that the sperm of the three species differs in terms of external morphology (size and shape of the head) and in relation to the length of the intermediate piece.

Parâmetros qualitativos do sêmen e morfologia comparada da cabeça e peça intermediária do espermatozoide de pirapitinga (Piaractus brachypomus), pacu (Piaractus mesopotamicus) e tambaqui (Colossoma macropomum) / Qualitative semen parameters and comparative morphology of pirapitinga (Piaractus brachypomus), pacu (Piaractus mesopotamicus) and tambaqui (Colossoma macropomum) sperm

O objetivo deste trabalho foi avaliar os parâmetros qualitativos do sêmen e comparar a morfologia da cabeça do espermatozoide entre três espécies de peixes, Piaractus mesopotamicus, Piaractus brachypomus e Colossoma macropomum. As amostras de sêmen foram coletadas de nove animais, três machos de cada espécie, por método de reprodução induzida no mês de novembro de 2011. Os espermatozoides foram submetidos à análise de motilidade e estudo da morfologia em microscopia eletrônica de varredura. As taxas de motilidade das três espécies avaliadas foram acima de 90%, não diferindo entre elas. Porém, a duração de motilidade do sêmen de pacu foi superior as demais espécies. O comprimento da cabeça (CC) dos espermatozóides de pacu, Pirapitinga e Tambaqui foram, respectivamente, 2,43 ± 0,19 μm, 1,94 ± 0,07 μm e 2,44 ± 0,12 μm. A largura da cabeça (LC) foram, respectivamente, 1,45 ± 0,07 μm, 1,76 ± 0,07 μm e 1,56 ± 0,06 μm. O comprimento da peça intermediária foram, respectivamente, 1,00 ± 0,10 μm, 1,54 ± 0,08 μm e 2,07 ± 0,33 μm. Os resultados revelam que os espermatozoides das três espécies diferem entre si na morfologia externa quanto ao tamanho e formato da cabeça, e nas proporções de comprimento da peça intermediária.(AU)

The objective of this work was to evaluate qualitative semen parameters and to compare the morphology of the sperm head among three fish species, Piaractus mesopotamicus, Piaractus brachypomus and Colossoma macropomum. The semen samples were collected from nine animals, three males per species, by the induced reproduction method in November 2011. Sperm motility was evaluated and morphology was analyzed by scanning electron microscopy. The motility rates of the three species evaluated were higher than 90%, with no difference between species. However, the duration of sperm motility was longer for pacu semen compared to the other species. The head length was 2.43 ± 0.19, 1.94 ± 0.07 and 2.44 ± 0.12 μm for pacu, pirapitinga and tambaqui sperm, respectively. The head width was 1.45 ± 0.07, 1.76 ± 0.07 and 1.56 ± 0.06 μm, and the length of the intermediate piece was 1.0 ± 0.10, 1.54 ± 0.08 and 2.07 ± 0.33 μm, respectively. The results show that the sperm of the three species differs in terms of external morphology (size and shape of the head) and in relation to the length of the intermediate piece.(AU)

Pouteria torta: a native species of the Brazilian Cerrado as a bioindicator of glyphosate action / Pouteria torta: espécie native do Cerrado Brasileiro como bioindicadora da ação do glyphosate

In Brazil, the expansion of agricultural activity and the associated indiscriminate use of herbicides such as glyphosate is directly related to the loss of biodiversity in the Cerrado. The identification of plant species as bioindicators of herbicide action, especially species native to the area, can help in monitoring the impacts of xenobiotics in the remaining Cerrado. Thus, this study was designed to evaluate the possible use of the native Cerrado species Pouteria torta as a bioindicator of glyphosate action via changes in physiological performance. At 16 months after sowing, the effect of glyphosate was evaluated by applying the following doses: 0 (control), 25, 50, 100, 200, 400, 800, and 1200 g a.e. ha-¹. In response to glyphosate, P. torta exhibited reductions in photosynthesis and chloroplastid pigment content, as well as accumulation of shikimic acid and the occurrence of chlorosis and necrosis. These changes demonstrate the high sensitivity of P. torta to glyphosate and its potential for use as a bioindicator of this herbicide.(AU)

No Brasil, a expansão da atividade agrícola, aliada a utilização indiscriminada de herbicidas como o glyphosate, possui relação direta com a perda da biodiversidade no Cerrado. A identificação de espécies vegetais bioindicadoras da ação de herbicidas, particularmente as nativas do Cerrado, pode auxiliar em processos de monitoramento dos impactos desse xenobiótico nas remanescentes do Cerrado. Assim, este estudo foi projetado para avaliar o possível uso de Pouteria torta, espécie nativa do cerrado, como bioindicadora da ação do glyphosate via mudanças na sua performance fisiológica. Após 16 meses de semeadura, o efeito do glyphosate foi avaliado quando aplicadas as seguintes doses: 0 (controle), 25, 50, 100, 200, 400, 800 e 1200 g e. a. ha-1. Em reposta ao glyphosate, as plantas de P. torta apresentaram redução na sua performance do processo fotossintético e no conteúdo de pigmentos cloroplastídicos, além do acúmulo de ácido chiquímico e da ocorrência de cloroses e necroses. Essas alterações demonstram a alta sensibilidade de P. torta ao glyphosate, o que potencializa a sua utilização como bioindicadora da ação desse herbicida.(AU)

Chelated Minerals and Limestone Particle Sizes on Performance and Bone Quality of Brown-Egg Layers

This study aimed to evaluate productive performance, egg quality, and bone quality of commercial brown-egg layers fed diets containing organic and inorganic minerals and three limestone particle sizes. A total of 288 birds at 64 weeks of age were distributed in a completely randomized design in a 2 × 3 factorial arrangement (two mineral sources and three limestone particle sizes) and eight replicates. The experiment lasted 112 days, divided into four periods. The following treatments were tested: T1 = inorganic minerals + 100% fine limestone; T2 = inorganic minerals + 50% fine limestone + 50% coarse limestone; T3 = inorganic minerals + 100% coarse limestone; T4 = inorganic + organic minerals + 100% fine limestone; T5 = inorganic + organic minerals + 50% fine limestone + 50% coarse limestone; and T6 = inorganic + organic minerals +100% coarse limestone. There was no significant interaction effect between the studied factors on any of the variables. No significant effect of the types of mineral mixes or limestone particle sizes were observed on the performance, egg quality, or bone quality variables evaluated. In conclusion, organic or inorganic minerals associated with limestone in fine, medium, or coarse particle sizes can be used in diets for brown-egg layers without affecting their productive performance, egg quality, or bone quality.(AU)

Chelated Minerals and Limestone Particle Sizes on Performance and Bone Quality of Brown-Egg Layers

This study aimed to evaluate productive performance, egg quality, and bone quality of commercial brown-egg layers fed diets containing organic and inorganic minerals and three limestone particle sizes. A total of 288 birds at 64 weeks of age were distributed in a completely randomized design in a 2 × 3 factorial arrangement (two mineral sources and three limestone particle sizes) and eight replicates. The experiment lasted 112 days, divided into four periods. The following treatments were tested: T1 = inorganic minerals + 100% fine limestone; T2 = inorganic minerals + 50% fine limestone + 50% coarse limestone; T3 = inorganic minerals + 100% coarse limestone; T4 = inorganic + organic minerals + 100% fine limestone; T5 = inorganic + organic minerals + 50% fine limestone + 50% coarse limestone; and T6 = inorganic + organic minerals +100% coarse limestone. There was no significant interaction effect between the studied factors on any of the variables. No significant effect of the types of mineral mixes or limestone particle sizes were observed on the performance, egg quality, or bone quality variables evaluated. In conclusion, organic or inorganic minerals associated with limestone in fine, medium, or coarse particle sizes can be used in diets for brown-egg layers without affecting their productive performance, egg quality, or bone quality.

Desempenho produtivo e características de carcaça de cordeiros Rabo Largo puro e cruzados com Santa Inês / Performance and carcass traits of Rabo Largo lambs and crossbreeding with Santa Inês

Objetivou-se avaliar o efeito do cruzamento entre ovinos nativos no desempenho e características da carcaça. Foram utilizados vinte cordeiros, machos, inteiros, com idade inicial de quatro meses, e peso médio inicial 19,0±5,11, distribuídos em dois grupos genéticos: Rabo Largo (RL) e Santa Inês x Rabo Largo (F1SI x RL), em um delineamento inteiramente ao acaso. Os animais foram terminados em regime de confinamento durante 70 dias. Os cordeiros F1SI x RL apresentaram maiores pesos ao desmame, ao abate, do corpo vazio, das carcaças quente e fria, e maior rendimento biológico. Verificaram maiores valores para os parâmetros de morfometria da carcaça para animais F1SI x RL comparados aos animais Rabo Largo (P<0,05). Os pesos da hemi-carcaça e dos cortes comerciais foram superiores para cordeiros F1SI x RL. Nas medidas obtidas no músculo Longissimus dorsi, os cordeiros Rabo Largo apresentaram valores superiores para espessura de gordura subcutânea. A utilização da raça Santa Inês como base paterna acarreta em melhorias nas características de carcaça de cordeiros Rabo Largo podendo ser indicado em sistemas de produção de carne ovina.(AU)

The aim of study was to evaluate the effect of crossbreeding among native sheep on performance and traits carcass. Twenty intact lambs, male, at four months of age, with an initial weight of 19.0±5.11kg, were distributed in two groups genetics: Rabo Largo (RL) e Santa Inês x Rabo Largo (F1SI x RL), in a completely randomized design. The animals were finished in feedlot by 70 days. The lambs F1SI x RL showed higher weight at weaning, to slaughter, of the empty body, and hot and cold carcass, and increased biological yield. Higher values for carcass morphometry in F1SI x RL animals were found in comparison to Rabo Largo animals (P<0.05). The weight of half-carcass and the commercial cuts were higher for lambs. On the measurements obtained in muscle Longissimus dorsi, Rabo Largo lambs showed higher values for subcutaneous fat thickness. The use of the breed Santa Inês like base paternal may bring in improvements in lambs's carcass characteristics Rabo Largo and may be recommended in sheep meat production systems.(AU)

Desempenho produtivo e características de carcaça de cordeiros Rabo Largo puro e cruzados com Santa Inês / Performance and carcass traits of Rabo Largo lambs and crossbreeding with Santa Inês

Objetivou-se avaliar o efeito do cruzamento entre ovinos nativos no desempenho e características da carcaça. Foram utilizados vinte cordeiros, machos, inteiros, com idade inicial de quatro meses, e peso médio inicial 19,0±5,11, distribuídos em dois grupos genéticos: Rabo Largo (RL) e Santa Inês x Rabo Largo (F1SI x RL), em um delineamento inteiramente ao acaso. Os animais foram terminados em regime de confinamento durante 70 dias. Os cordeiros F1SI x RL apresentaram maiores pesos ao desmame, ao abate, do corpo vazio, das carcaças quente e fria, e maior rendimento biológico. Verificaram maiores valores para os parâmetros de morfometria da carcaça para animais F1SI x RL comparados aos animais Rabo Largo (P<0,05). Os pesos da hemi-carcaça e dos cortes comerciais foram superiores para cordeiros F1SI x RL. Nas medidas obtidas no músculo Longissimus dorsi, os cordeiros Rabo Largo apresentaram valores superiores para espessura de gordura subcutânea. A utilização da raça Santa Inês como base paterna acarreta em melhorias nas características de carcaça de cordeiros Rabo Largo podendo ser indicado em sistemas de produção de carne ovina.(AU)

The aim of study was to evaluate the effect of crossbreeding among native sheep on performance and traits carcass. Twenty intact lambs, male, at four months of age, with an initial weight of 19.0±5.11kg, were distributed in two groups genetics: Rabo Largo (RL) e Santa Inês x Rabo Largo (F1SI x RL), in a completely randomized design. The animals were finished in feedlot by 70 days. The lambs F1SI x RL showed higher weight at weaning, to slaughter, of the empty body, and hot and cold carcass, and increased biological yield. Higher values for carcass morphometry in F1SI x RL animals were found in comparison to Rabo Largo animals (P<0.05). The weight of half-carcass and the commercial cuts were higher for lambs. On the measurements obtained in muscle Longissimus dorsi, Rabo Largo lambs showed higher values for subcutaneous fat thickness. The use of the breed Santa Inês like base paternal may bring in improvements in lambs's carcass characteristics Rabo Largo and may be recommended in sheep meat production systems.(AU)

Environmental enrichment on the behavior and welfare of cockatiels (Nymphicus hollandicus) / Enriquecimento ambiental no comportamento e no bem-estar de calopsitas (Nymphicus hollandicus)

This study evaluated the influence of physical environmental enrichment on the behavior of cockatiels (Nymphicus hollandicus). Eighteen birds, nine males and nine females, were monitored in environments enriched with wooden sticks and bead rings and in non-enriched environments, in a completely randomized design. Behavioral categories were grouped into locomotion, maintenance, rest, feed, undesirable activities, and interaction with environmental enrichment. Data were analyzed using descriptive analysis of behaviors to produce the ethogram and percentage comparison of frequency values for behavior analysis. Environmental enrichment positively influenced behavioral categories and some behavioral activities of birds.(AU)

Objetivou-se, com o presente trabalho, avaliar a influência do enriquecimento ambiental físico sobre o comportamento de calopsitas (Nymphicus hollandicus). Foram monitoradas 18 aves, sendo nove machos e nove fêmeas, em ambientes enriquecidos com tocos de madeira e argola de miçangas e sem enriquecimento ambiental. O delineamento foi inteiramente ao acaso. As categorias comportamentais foram agrupadas em locomoção, manutenção, repouso, alimentação, atividades indesejáveis e interação com o enriquecimento ambiental. Os dados foram examinados por meio da análise descritiva dos comportamentos para elaboração do etograma e comparação percentual das frequências observadas para análise dos comportamentos. Foi observada influência positiva do enriquecimento ambiental sobre as categorias comportamentais e algumas atividades comportamentais das aves.(AU)

Environmental enrichment on the behavior and welfare of cockatiels (Nymphicus hollandicus) / Enriquecimento ambiental no comportamento e no bem-estar de calopsitas (Nymphicus hollandicus)

This study evaluated the influence of physical environmental enrichment on the behavior of cockatiels (Nymphicus hollandicus). Eighteen birds, nine males and nine females, were monitored in environments enriched with wooden sticks and bead rings and in non-enriched environments, in a completely randomized design. Behavioral categories were grouped into locomotion, maintenance, rest, feed, undesirable activities, and interaction with environmental enrichment. Data were analyzed using descriptive analysis of behaviors to produce the ethogram and percentage comparison of frequency values for behavior analysis. Environmental enrichment positively influenced behavioral categories and some behavioral activities of birds.(AU)

Objetivou-se, com o presente trabalho, avaliar a influência do enriquecimento ambiental físico sobre o comportamento de calopsitas (Nymphicus hollandicus). Foram monitoradas 18 aves, sendo nove machos e nove fêmeas, em ambientes enriquecidos com tocos de madeira e argola de miçangas e sem enriquecimento ambiental. O delineamento foi inteiramente ao acaso. As categorias comportamentais foram agrupadas em locomoção, manutenção, repouso, alimentação, atividades indesejáveis e interação com o enriquecimento ambiental. Os dados foram examinados por meio da análise descritiva dos comportamentos para elaboração do etograma e comparação percentual das frequências observadas para análise dos comportamentos. Foi observada influência positiva do enriquecimento ambiental sobre as categorias comportamentais e algumas atividades comportamentais das aves.(AU)

Caracterização dos consumidores de peixe do Município de Lavras, Minas Gerais / Characterization of fish consumers in the town of Lavras, Minas Gerais

Objetivou-se com este trabalho avaliar e caracterizar o perfil de consumo de peixe na cidade de Lavras, MG. Para a obtenção dos dados foi realizada uma pesquisa descritivo-quantitativa. A técnica de coleta de dados utilizada foi o questionário estruturado, elaborado com perguntas e respostas previamente formuladas. O questionário foi aplicado de março a maio de 2014 e voltado à pessoa de referência do domicílio. Foram amostrados 402 domicílios. Para a análise dos dados, foi utilizada a técnica de análise de distribuição de frequências, através do programa estatístico SPSS versão 12.0. Como resultado, observou-se que a carne de peixe está em quarto lugar no ranking de preferência de consumo das famílias na cidade de Lavras, perdendo para a carne bovina, aves e suína. Constatou-se também que normalmente o peixe é adquirido na apresentação filé e que o consumo é eventual nos domicílios. O preço representou um fator importante para escolha da carne. A maioria dos entrevistados prefere consumir o peixe frito. A espécie mais apreciada pelos consumidores da cidade de Lavras foi o Dourado. A maioria dos entrevistados costuma comprar menos de 2,0 kg/domicílio/ano...(AU)

The objective of this study was to evaluate and characterize the fish consumption profile in the town of Lavras, Minas Gerais. A descriptive-quantitative study was conducted. A structured questionnaire elaborated using previously formulated questions and answers was used for data collection. The questionnaire was applied between March and May 2014 to the household reference person. A total of 402 households were sampled. The data were analyzed by analysis of the distribution of frequencies using the SPSS program. The results showed that fish meat occupied fourth place in the preference of household consumption in the town of Lavras, after beef, poultry and pork meat. It was also observed that fish is usually purchased as fillet and is consumed occasionally in the households. The price is an important factor for the choice of meat. Most respondents prefer to consume fried fish. The species most appreciated by consumers in the town of Lavras is Dourado. Most respondents usually buy less than 2.0 kg/household/year...(AU)

Caracterização dos consumidores de peixe do Município de Lavras, Minas Gerais / Characterization of fish consumers in the town of Lavras, Minas Gerais

Objetivou-se com este trabalho avaliar e caracterizar o perfil de consumo de peixe na cidade de Lavras, MG. Para a obtenção dos dados foi realizada uma pesquisa descritivo-quantitativa. A técnica de coleta de dados utilizada foi o questionário estruturado, elaborado com perguntas e respostas previamente formuladas. O questionário foi aplicado de março a maio de 2014 e voltado à pessoa de referência do domicílio. Foram amostrados 402 domicílios. Para a análise dos dados, foi utilizada a técnica de análise de distribuição de frequências, através do programa estatístico SPSS versão 12.0. Como resultado, observou-se que a carne de peixe está em quarto lugar no ranking de preferência de consumo das famílias na cidade de Lavras, perdendo para a carne bovina, aves e suína. Constatou-se também que normalmente o peixe é adquirido na apresentação filé e que o consumo é eventual nos domicílios. O preço representou um fator importante para escolha da carne. A maioria dos entrevistados prefere consumir o peixe frito. A espécie mais apreciada pelos consumidores da cidade de Lavras foi o Dourado. A maioria dos entrevistados costuma comprar menos de 2,0 kg/domicílio/ano...

The objective of this study was to evaluate and characterize the fish consumption profile in the town of Lavras, Minas Gerais. A descriptive-quantitative study was conducted. A structured questionnaire elaborated using previously formulated questions and answers was used for data collection. The questionnaire was applied between March and May 2014 to the household reference person. A total of 402 households were sampled. The data were analyzed by analysis of the distribution of frequencies using the SPSS program. The results showed that fish meat occupied fourth place in the preference of household consumption in the town of Lavras, after beef, poultry and pork meat. It was also observed that fish is usually purchased as fillet and is consumed occasionally in the households. The price is an important factor for the choice of meat. Most respondents prefer to consume fried fish. The species most appreciated by consumers in the town of Lavras is Dourado. Most respondents usually buy less than 2.0 kg/household/year...

Initiation and s uppression of sperm motility is osmolality dependent in two South American fish species: streaked prochilod (Prochilodus lineatus) and piracanjuba(Brycon orbignyanus)

Solutions to induce or suppress the initiation of sperm motility in fish ha ve been used to improve reproductive success during artificial fertilization and preservation techniques . The aim of the present study was to evaluate the effects of three solutions (NaCl, glucose , and BTS™) - each prepared with 10 different osmolalities - on the initiation and suppression of fresh sperm motility in Prochilodus lineat us and Brycon orbignyanus . Sperm was diluted in each of the 30 solution s and immediately observed under a light microscope to determine which solution s trigger ed or suppress ed the initiation of sperm motility. When present, motility rate ( % motile sperm ) w as determined at 0, 30 , and 120 s post - activation and the motility quality score ranging from 0 ( no movement ) to 5 ( rapidly swimming sperm) was determined at 0 and 30 s post - activation . Osmolality , but not solution composition , significantly affected both motility rate and quality score . Solutions at osmolali ties up to 270 mOsm/kg in P. lineatus and up to 180 mOsm/kg in B. orbignyanus induced motility in at least 60% of sperm , with a minimum quality score of 3.0 , and were therefore classified as activating agents. The greatest motility at 0 , 30 , and 120 s post - activation was observed with solutions ranging from 135 to 225 mOsm/kg for P. lineatus and at 135 mOsm/kg for B. orbignyanus . On the other hand, solutions ranging from 360 to 450 mOsm/kg in P. lineatus and 270 to 450 mOsm/kg in B. orbignyanus suppressed motility in at least 95% of sperm and were classified as immobilizing media . The osmolality of the surrounding medium is the key factor in the initiation or suppression of sperm motility in P. lineatus a nd B. orbignyanu.

Initiation and s uppression of sperm motility is osmolality dependent in two South American fish species: streaked prochilod (Prochilodus lineatus) and piracanjuba(Brycon orbignyanus)

Solutions to induce or suppress the initiation of sperm motility in fish ha ve been used to improve reproductive success during artificial fertilization and preservation techniques . The aim of the present study was to evaluate the effects of three solutions (NaCl, glucose , and BTS™) - each prepared with 10 different osmolalities - on the initiation and suppression of fresh sperm motility in Prochilodus lineat us and Brycon orbignyanus . Sperm was diluted in each of the 30 solution s and immediately observed under a light microscope to determine which solution s trigger ed or suppress ed the initiation of sperm motility. When present, motility rate ( % motile sperm ) w as determined at 0, 30 , and 120 s post - activation and the motility quality score ranging from 0 ( no movement ) to 5 ( rapidly swimming sperm) was determined at 0 and 30 s post - activation . Osmolality , but not solution composition , significantly affected both motility rate and quality score . Solutions at osmolali ties up to 270 mOsm/kg in P. lineatus and up to 180 mOsm/kg in B. orbignyanus induced motility in at least 60% of sperm , with a minimum quality score of 3.0 , and were therefore classified as activating agents. The greatest motility at 0 , 30 , and 120 s post - activation was observed with solutions ranging from 135 to 225 mOsm/kg for P. lineatus and at 135 mOsm/kg for B. orbignyanus . On the other hand, solutions ranging from 360 to 450 mOsm/kg in P. lineatus and 270 to 450 mOsm/kg in B. orbignyanus suppressed motility in at least 95% of sperm and were classified as immobilizing media . The osmolality of the surrounding medium is the key factor in the initiation or suppression of sperm motility in P. lineatus a nd B. orbignyanu.(AU)

Eficácia do glyphosate e 2, 4-d no controle de Commelina villosa / Efficacy of glyphosate and 2, 4-D in control of Commelina villosa

O objetivo do presente trabalho foi avaliar a eficácia de glyphosate e 2,4-D, isolados e em mistura, no controle de Commelina villosa. Foram estudadas duas metodologias de avaliação de absorção de herbicidas em oito intervalos de tempo para a lavagem (simulando chuva após a aplicação) e corte (simulando abortamento, como estratégia de defesa) das folhas: 2, 4, 6, 8, 12, 24 e 48 horas após a aplicação dos herbicidas, além de um tratamento sem lavagem ou corte das folhas, em delineamento experimental inteiramente casualizado com quatro repetições, dispostos em um esquema fatorial 3 x 7 + 1 (três herbicidas x sete períodos – horas após a aplicação). Os herbicidas e doses testados foram: glyphosate (1.440 g ha-1), 2,4-D (720 g ha-1) e a mistura glyphosate + 2,4-D (1.080 + 720 g ha-1). A simulação de chuva interferiu de forma negativa no controle das plantas com o herbicida glyphosate. O controle com o herbicida 2,4-D foi influenciado apenas no período de 2 horas. Os períodos de simulação de chuva não influenciaram no controle das plantas com a mistura de glyphosate + 2,4-D. Para o estudo com corte das folhas tratadas, todos os tratamentos independente do período para corte das folhas foram influenciados de forma negativa no controle, sendo que as plantas apresentaram rebrotas quando tratadas com o herbicida 2,4-D isolado.

Efficacy of glyphosate and 2,4-d in the control of Commelina Villosa. The aim of this study was to evaluate the efficacy of glyphosate and 2,4-D alone and in combination, in the control of Commelina villosa. We studied two methodologies for evaluating herbicide absorption in eight time intervals for washing (simulating rainfall after application) and cutting of leaves (simulating abortion as a defense strategy): 2, 4, 6, 8, 12, 24 and 48 hours after herbicide application, and a treatment without washing or cutting the leaves in a completely randomized design with four replications in a 3 x 7 + 1 factorial design (three herbicides x seven periods – hours after application). Herbicides and doses tested were: glyphosate (1,440 g ha-1), 2,4-D (720 g ha-1) and a mixture of glyphosate + 2,4-D (1,080 + 720 g ha-1). The simulation of rain interfered negatively in the plant control with glyphosate. The control with the herbicide 2,4-D was affected only for the period of 2 hours. Periods of rain simulation did not influence the control of plants with a mixture of glyphosate + 2,4-D. For the study with the cutting of treated leaves, all treatments regardless of the period of cutting the leaves were influenced negatively in terms of plant control, the plants showing regrowth when treated with 2,4-D alone.

Eficácia do glyphosate e 2, 4-d no controle de Commelina villosa / Efficacy of glyphosate and 2, 4-D in control of Commelina villosa

O objetivo do presente trabalho foi avaliar a eficácia de glyphosate e 2,4-D, isolados e em mistura, no controle de Commelina villosa. Foram estudadas duas metodologias de avaliação de absorção de herbicidas em oito intervalos de tempo para a lavagem (simulando chuva após a aplicação) e corte (simulando abortamento, como estratégia de defesa) das folhas: 2, 4, 6, 8, 12, 24 e 48 horas após a aplicação dos herbicidas, além de um tratamento sem lavagem ou corte das folhas, em delineamento experimental inteiramente casualizado com quatro repetições, dispostos em um esquema fatorial 3 x 7 + 1 (três herbicidas x sete períodos horas após a aplicação). Os herbicidas e doses testados foram: glyphosate (1.440 g ha-1), 2,4-D (720 g ha-1) e a mistura glyphosate + 2,4-D (1.080 + 720 g ha-1). A simulação de chuva interferiu de forma negativa no controle das plantas com o herbicida glyphosate. O controle com o herbicida 2,4-D foi influenciado apenas no período de 2 horas. Os períodos de simulação de chuva não influenciaram no controle das plantas com a mistura de glyphosate + 2,4-D. Para o estudo com corte das folhas tratadas, todos os tratamentos independente do período para corte das folhas foram influenciados de forma negativa no controle, sendo que as plantas apresentaram rebrotas quando tratadas com o herbicida 2,4-D isolado.(AU)

Efficacy of glyphosate and 2,4-d in the control of Commelina Villosa. The aim of this study was to evaluate the efficacy of glyphosate and 2,4-D alone and in combination, in the control of Commelina villosa. We studied two methodologies for evaluating herbicide absorption in eight time intervals for washing (simulating rainfall after application) and cutting of leaves (simulating abortion as a defense strategy): 2, 4, 6, 8, 12, 24 and 48 hours after herbicide application, and a treatment without washing or cutting the leaves in a completely randomized design with four replications in a 3 x 7 + 1 factorial design (three herbicides x seven periods hours after application). Herbicides and doses tested were: glyphosate (1,440 g ha-1), 2,4-D (720 g ha-1) and a mixture of glyphosate + 2,4-D (1,080 + 720 g ha-1). The simulation of rain interfered negatively in the plant control with glyphosate. The control with the herbicide 2,4-D was affected only for the period of 2 hours. Periods of rain simulation did not influence the control of plants with a mixture of glyphosate + 2,4-D. For the study with the cutting of treated leaves, all treatments regardless of the period of cutting the leaves were influenced negatively in terms of plant control, the plants showing regrowth when treated with 2,4-D alone.(AU)

Tipificação genética, através da técnica de spoligotyping, de isolados de Mycobacterium bovis em animais abatidos na região metropolitana de Salvador, Bahia, Brasil / Use of spoligotyping for genetic typing of Mycobacterium bovis isolates from slaughtered animals in the metropolitan area of Salvador, Bahia, Brazil

A tuberculose é uma enfermidade infecciosa crônica, que afeta mamíferos e aves e constitui um sério problema de saúde pública e animal. Objetivando realizar um levantamento molecular da enfermidade em bovinos abatidos em matadouros frigoríficos no Estado da Bahia, Brasil, foram analisadas as lesões pulmonares e de linfonodos mediastínicos de 43 carcaças de animais abatidos em três matadouros-frigoríficos localizados na Região Metropolitana de Salvador, Bahia. Sete isolados de Mycobacterium bovis foram identificados, através da técnica do spolygotyping, e discriminados em três diferentes espoligotipos (SB1055, SB0120 e SB0268) descritos no Brasil e em diversas áreas do mundo. Os resultados indicam que o método de diagnóstico utilizado pode contribuir para a criação de uma base de dados para o estudo epidemiológico da tuberculose bovina no Estado da Bahia.

Tuberculosis is an infectious chronic disease that affects mammals and birds and constitutes a serious problem for public and animal health. Pulmonary and mediastinic lymph node lesions of 43 animals slaughtered in 3 slaughterhouses in the metropolitan region of the city of Salvador, Bahia, Brazil, were analyzed with the objective of obtaining a molecular survey of the disease in bovines slaughtered in slaughterhouses in the state. Seven isolates ofMycobacterium bovis were identified through the spoligotyping technique and classified into 3 different spoligotypes (SB1055, SB0120, SB0268), described in Brazil and in many areas worldwide. The results indicate that the diagnostic method utilized may contribute to the creation of a database for the epidemiologic study of bovine tuberculosis in the state of Bahia.

TIPIFICAÇÃO GENÉTICA, ATRAVÉS DA TÉCNICA DE SPOLIGOTYPING, DE ISOLADOS DE MYCOBACTERIUM BOVIS EM ANIMAIS ABATIDOS NA REGIÃO METROPOLITANA DE SALVADOR, BAHIA, BRASIL

ABSTRACT Tuberculosis is an infectious chronic disease that affects mammals and birds and constitutes a serious problem for public and animal health. Pulmonary and mediastinic lymph node lesions of 43 animals slaughtered in 3 slaughterhouses in the metropolitan region of the city of Salvador, Bahia, Brazil, were analyzed with the objective of obtaining a molecular survey of the disease in bovines slaughtered in slaughterhouses in the state. Seven isolates ofMycobacterium bovis were identified through the spoligotyping technique and classified into 3 different spoligotypes (SB1055, SB0120, SB0268), described in Brazil and in many areas worldwide. The results indicate that the diagnostic method utilized may contribute to the creation of a database for the epidemiologic study of bovine tuberculosis in the state of Bahia.

RESUMO A tuberculose é uma enfermidade infecciosa crônica, que afeta mamíferos e aves e constitui um sério problema de saúde pública e animal. Objetivando realizar um levantamento molecular da enfermidade em bovinos abatidos em matadouros frigoríficos no Estado da Bahia, Brasil, foram analisadas as lesões pulmonares e de linfonodos mediastínicos de 43 carcaças de animais abatidos em três matadouros-frigoríficos localizados na Região Metropolitana de Salvador, Bahia. Sete isolados de Mycobacterium bovis foram identificados, através da técnica do spolygotyping, e discriminados em três diferentes espoligotipos (SB1055, SB0120 e SB0268) descritos no Brasil e em diversas áreas do mundo. Os resultados indicam que o método de diagnóstico utilizado pode contribuir para a criação de uma base de dados para o estudo epidemiológico da tuberculose bovina no Estado da Bahia.