Resumo
Species of Spirostomum Ehrenberg, 1838 are widely used as model organisms in ecological studies of environmental impacts and symbioses between ciliates and human pathogenic bacteria. However, the taxonomy of this genus is confused by the superficiality of the morphological descriptions of its included species, and the use of only a few characters for their differentiation. The present study provides details of total infraciliature, nuclear apparatus, morphometric data and 18S rDNA gene sequences of Spirostomum teres Claparède & Lachmann, 1858 and Spirostomum minus Roux, 1901, isolated from a sewage treatment plant and a freshwater lake in the city of Rio de Janeiro, Brazil, respectively. For the morphological descriptions of S. teres and S. minus, living cells were observed using bright-field and differential interference contrast (DIC) microscopy, the total infraciliature and nuclear apparatus were revealed by staining with protargol, and ciliary patterns were observed also with scanning electron microscopy (SEM). The complete sequences of the 18S rDNA of S. teres and S. minus were obtained using eukaryotic universal primers, and then compared with sequences of other species and populations of Spirostomum deposited in the GenBank database. Living S. minus measured 400-800 µm in length and 55-115 µm in width, with the following characteristics: adoral zone of membranelles approximately 112 µm long; inconspicuous paroral kinety; 30-40 kineties in somatic ciliature; moniliform macronucleus with 9-25 nodes, approximately 12 micronuclei; single and posterior contractile vacuole; and yellow-brown cytoplasm. Living and fully extended S. teres measured approximately 250 µm in length and 65 ìm in width, with the following characteristics: adoral zone of membranelles approximately 92 µm long; approximately 30 somatic kineties; compact macronucleus, approximately five micronuclei; macronuclear groove present; single and posterior contractile vacuole; and colorless cytoplasm. Evidence from 18S rDNA sequences confirms the identification of S. teres and suggests the existence of cryptic species closely related to S. minus. The use of silver impregnation technique (protargol) allowed the observation and description of a greater number of characters in S. minus and S. teres, thus assisting the research that require identification of these species.
Resumo
During an ecological study of the epibiotic relationship between ciliate protists and Pomacea figulina (Spix, 1827) (Gastropoda, Ampullariidae), originating from an urban stream in southeast Brazil, a high infestation by the peritrich ciliate Carchesium polypinum (Linnaeus, 1758) Ehrenberg, 1830 (Ciliophora, Peritrichia) associated to the shell of one mollusc among 23 was observed. We provided a morphological and morphometric study of C. polypinum using observations of specimens in vivo, after protargol staining, and examined using scanning electron microscopy. The Brazilian-population of C. polypinum is characterized by: size of zooid in vivo 89 µm x 57 µm on average; colony regularly dichotomously branched with usually up to 40 zooids; macronucleus usually J-shaped; single contractile vacuole located in the upper third of body; myoneme not continuous throughout the colony; stalks contract despite the discontinuity of their individual myonemes; polykinety comprises three peniculi, each consisting of three kineties. The high infestation showed here could be related to the preference for eutrophic environments showed by C. polypinum and suggested that ciliate epibionts may be ecologically important in aquatic habitats.
Resumo
During an ecological study of the epibiotic relationship between ciliate protists and Pomacea figulina (Spix, 1827) (Gastropoda, Ampullariidae), originating from an urban stream in southeast Brazil, a high infestation by the peritrich ciliate Carchesium polypinum (Linnaeus, 1758) Ehrenberg, 1830 (Ciliophora, Peritrichia) associated to the shell of one mollusc among 23 was observed. We provided a morphological and morphometric study of C. polypinum using observations of specimens in vivo, after protargol staining, and examined using scanning electron microscopy. The Brazilian-population of C. polypinum is characterized by: size of zooid in vivo 89 µm x 57 µm on average; colony regularly dichotomously branched with usually up to 40 zooids; macronucleus usually J-shaped; single contractile vacuole located in the upper third of body; myoneme not continuous throughout the colony; stalks contract despite the discontinuity of their individual myonemes; polykinety comprises three peniculi, each consisting of three kineties. The high infestation showed here could be related to the preference for eutrophic environments showed by C. polypinum and suggested that ciliate epibionts may be ecologically important in aquatic habitats.
Resumo
During an ecological study of the epibiotic relationship between ciliate protists and Pomacea figulina (Spix, 1827) (Gastropoda, Ampullariidae), originating from an urban stream in southeast Brazil, a high infestation by the peritrich ciliate Carchesium polypinum (Linnaeus, 1758) Ehrenberg, 1830 (Ciliophora, Peritrichia) associated to the shell of one mollusc among 23 was observed. We provided a morphological and morphometric study of C. polypinum using observations of specimens in vivo, after protargol staining, and examined using scanning electron microscopy. The Brazilian-population of C. polypinum is characterized by: size of zooid in vivo 89 µm x 57 µm on average; colony regularly dichotomously branched with usually up to 40 zooids; macronucleus usually J-shaped; single contractile vacuole located in the upper third of body; myoneme not continuous throughout the colony; stalks contract despite the discontinuity of their individual myonemes; polykinety comprises three peniculi, each consisting of three kineties. The high infestation showed here could be related to the preference for eutrophic environments showed by C. polypinum and suggested that ciliate epibionts may be ecologically important in aquatic habitats.
Resumo
In samples of raw sewage collected from a sewage treatment plant in Rio de Janeiro (ETE-Penha), we found populations of two species, Deviata estevesi Paiva & Silva-Neto, 2005 and Deviata brasiliensis sp. nov. The organisms were studied in vivo under phase contrast microscopy, differential interference contrast (DIC), and after protargol-impregnation. The population of D. estevesi exhibited more extensive variation in cirral pattern than previously described. The interphasic organisms of new species D. brasiliensis sp. nov. are distinguishable from their congeners based on a series of morphometric features: cirral row R3 usually presents 1-3 cirri behind the right frontal cirrus, on average there are four macronuclear nodules, and, during morphogenesis of cell division, primordium V of the proter originates from the anterior region of cirral row R5 instead of row R6, as in the type species D. abbrevescens Eigner, 1995. In D. estevesi, the ventral cirral rows replicate by within-row primordia, which develop independently for the proter and for the opisthe, suggesting that it belongs to or is closely related to Parastrongylidium, hence the combination P. estevesi comb. nov. is established.
Resumo
In samples of raw sewage collected from a sewage treatment plant in Rio de Janeiro (ETE-Penha), we found populations of two species, Deviata estevesi Paiva & Silva-Neto, 2005 and Deviata brasiliensis sp. nov. The organisms were studied in vivo under phase contrast microscopy, differential interference contrast (DIC), and after protargol-impregnation. The population of D. estevesi exhibited more extensive variation in cirral pattern than previously described. The interphasic organisms of new species D. brasiliensis sp. nov. are distinguishable from their congeners based on a series of morphometric features: cirral row R3 usually presents 1-3 cirri behind the right frontal cirrus, on average there are four macronuclear nodules, and, during morphogenesis of cell division, primordium V of the proter originates from the anterior region of cirral row R5 instead of row R6, as in the type species D. abbrevescens Eigner, 1995. In D. estevesi, the ventral cirral rows replicate by within-row primordia, which develop independently for the proter and for the opisthe, suggesting that it belongs to or is closely related to Parastrongylidium, hence the combination P. estevesi comb. nov. is established.
Resumo
In samples of raw sewage collected from a sewage treatment plant in Rio de Janeiro (ETE-Penha), we found populations of two species, Deviata estevesi Paiva & Silva-Neto, 2005 and Deviata brasiliensis sp. nov. The organisms were studied in vivo under phase contrast microscopy, differential interference contrast (DIC), and after protargol-impregnation. The population of D. estevesi exhibited more extensive variation in cirral pattern than previously described. The interphasic organisms of new species D. brasiliensis sp. nov. are distinguishable from their congeners based on a series of morphometric features: cirral row R3 usually presents 1-3 cirri behind the right frontal cirrus, on average there are four macronuclear nodules, and, during morphogenesis of cell division, primordium V of the proter originates from the anterior region of cirral row R5 instead of row R6, as in the type species D. abbrevescens Eigner, 1995. In D. estevesi, the ventral cirral rows replicate by within-row primordia, which develop independently for the proter and for the opisthe, suggesting that it belongs to or is closely related to Parastrongylidium, hence the combination P. estevesi comb. nov. is established.