Resumo
Studies on essential oils of Brazilian propolis indicated the existence of significant differences in their qualitative composition. These differences may be influenced by the seasons and the production method. This work aims to evaluate the composition of essential oil from propolis produced in different seasons and collection method (shim, smart propolis collector and plastic screen) on propolis collected by bees Apis mellifera. Fifteen hives of africanized bees Apis mellifera, housed in standard Langstroth hives, were randomly distributed and managed for the exclusively production of propolis. Before the beginning of the experiment, the nests were standardized as to the number of frames and creates. Five bee hives were randomly selected for each method of propolis production in the following treatments: T1- smart propolis collector (SPC); T2- plastic screen and T3- shim. The propolis were collected monthly in each treatment, and were cleaned, mixed and stored until the moment of the analysis of extraction of essential oil. Essential oil extraction was performed by hydrodistillation using Clevenger apparatus in a period of six hours. The oil extracted was split into aliquots and stored at 4-6C, and was subjected to chemical analysis by means of mass spectrometer coupled to a gas chromatograph (GC-MS), SHIMAZU brand, model QP5050A. The identifications of substances were made
O artigo não apresenta resumo em português.
Resumo
Studies on essential oils of Brazilian propolis indicated the existence of significant differences in their qualitative composition. These differences may be influenced by the seasons and the production method. This work aims to evaluate the composition of essential oil from propolis produced in different seasons and collection method (shim, smart propolis collector and plastic screen) on propolis collected by bees Apis mellifera. Fifteen hives of africanized bees Apis mellifera, housed in standard Langstroth hives, were randomly distributed and managed for the exclusively production of propolis. Before the beginning of the experiment, the nests were standardized as to the number of frames and creates. Five bee hives were randomly selected for each method of propolis production in the following treatments: T1- smart propolis collector (SPC); T2- plastic screen and T3- shim. The propolis were collected monthly in each treatment, and were cleaned, mixed and stored until the moment of the analysis of extraction of essential oil. Essential oil extraction was performed by hydrodistillation using Clevenger apparatus in a period of six hours. The oil extracted was split into aliquots and stored at 4-6C, and was subjected to chemical analysis by means of mass spectrometer coupled to a gas chromatograph (GC-MS), SHIMAZU brand, model QP5050A. The identifications of substances were made
O artigo não apresenta resumo em português.