Evaluation of Physiological Parameters of Broilers with Dorsal Cranial Myopathy

There are several causes of carcass condemnation in poultry processing plants, including dorsal cranial myopathy (DCM), in which the anterior latissimus dorsi (ALD) muscle is affected. DCM etiology has not been elucidated yet, but this lesion impairs the visual quality of carcasses and causes economic losses due to downgrading and condemnation. The effects of this lesion on the systemic health of broilers are still unknown. In this context, the objective of this study was to evaluate muscle injury and systemic health indicators in broilers presenting or not DCM. The following parameters were evaluated: complete blood count (CBC), total protein, albumin, globulin, cholesterol, lactate, and glucose serum levels, aspartate aminotransferase (AST) and creatine kinase (CK) serum activities, and breast muscle pH. Blood samples were collected from 800 42-day-old broilers before feed withdrawal and transportation. In the processing plant, 28 carcasses presented DCM during inspection, and 28 carcasses with no DCM were used as controls. Blood biochemical parameters were not significantly different between broilers with DCM and the controls, except for AST and CK, which activities were higher in the DCM group than in the control group, suggesting that the DCM does not affect the systemic health of the broilers.(AU)

Evaluation of Physiological Parameters of Broilers with Dorsal Cranial Myopathy

There are several causes of carcass condemnation in poultry processing plants, including dorsal cranial myopathy (DCM), in which the anterior latissimus dorsi (ALD) muscle is affected. DCM etiology has not been elucidated yet, but this lesion impairs the visual quality of carcasses and causes economic losses due to downgrading and condemnation. The effects of this lesion on the systemic health of broilers are still unknown. In this context, the objective of this study was to evaluate muscle injury and systemic health indicators in broilers presenting or not DCM. The following parameters were evaluated: complete blood count (CBC), total protein, albumin, globulin, cholesterol, lactate, and glucose serum levels, aspartate aminotransferase (AST) and creatine kinase (CK) serum activities, and breast muscle pH. Blood samples were collected from 800 42-day-old broilers before feed withdrawal and transportation. In the processing plant, 28 carcasses presented DCM during inspection, and 28 carcasses with no DCM were used as controls. Blood biochemical parameters were not significantly different between broilers with DCM and the controls, except for AST and CK, which activities were higher in the DCM group than in the control group, suggesting that the DCM does not affect the systemic health of the broilers.

Effect of Antimicrobials on Salmonella Spp. Strains Isolated from Poultry Processing Plants

The routine use of antimicrobials in animal production for the treatment of infections, disease prevention, or as growth promoters is a predisposing factor for the development and dissemination of antimicrobial resistance. In food industries, sanitizers are used for the control of microbial colonization, and their efficacy depends on contact time and on the dilution of the products used. The present study assessed the effect of 12 antimicrobials and four commercial sanitizers on 18 Salmonella spp. strains isolated from poultry processing plants. None of the evaluated antimicrobials was 100% effective against the tested Salmonella spp. strains; however, 94% of the isolates were susceptible to ciprofloxacin, 77% to amoxicillin + clavulanic acid and to ampicillin, and 72% to enrofloxacin, whereas 100% of the isolates were resistant to penicillin G, 16% to tetracycline, and 11% to sulfonamide. The tested Salmonella spp. strains were 100% inhibited by peracetic acid after five minutes of contact, 0.5% by quaternary ammonium after 15 minutes, and 85.7% by chlorhexidine after 15 minutes. The results indicate the importance of testing of efficacy of antimicrobials used in animal production and in public health to monitor their action and the development of resistance.

Effect of Antimicrobials on Salmonella Spp. Strains Isolated from Poultry Processing Plants

The routine use of antimicrobials in animal production for the treatment of infections, disease prevention, or as growth promoters is a predisposing factor for the development and dissemination of antimicrobial resistance. In food industries, sanitizers are used for the control of microbial colonization, and their efficacy depends on contact time and on the dilution of the products used. The present study assessed the effect of 12 antimicrobials and four commercial sanitizers on 18 Salmonella spp. strains isolated from poultry processing plants. None of the evaluated antimicrobials was 100% effective against the tested Salmonella spp. strains; however, 94% of the isolates were susceptible to ciprofloxacin, 77% to amoxicillin + clavulanic acid and to ampicillin, and 72% to enrofloxacin, whereas 100% of the isolates were resistant to penicillin G, 16% to tetracycline, and 11% to sulfonamide. The tested Salmonella spp. strains were 100% inhibited by peracetic acid after five minutes of contact, 0.5% by quaternary ammonium after 15 minutes, and 85.7% by chlorhexidine after 15 minutes. The results indicate the importance of testing of efficacy of antimicrobials used in animal production and in public health to monitor their action and the development of resistance.(AU)

Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat

Salmonella is traditionally identified by conventional microbiological tests, but the enumeration of this bacterium is not used on a routine basis. Methods such as the most probable number (MPN), which utilize an array of multiple tubes, are time-consuming and expensive, whereas miniaturized most probable number (mMPN) methods, which use microplates, can be adapted for the enumeration of bacteria, saving up time and materials. The aim of the present paper is to assess two mMPN methods for the enumeration of Salmonella sp in artificially-contaminated chicken meat samples. Microplates containing 24 wells (method A) and 96 wells (method B), both with peptone water as pre-enrichment medium and modified semi-solid Rappaport-Vassiliadis (MSRV) as selective enrichment medium, were used. The meat matrix consisted of 25g of autoclaved ground chicken breast contaminated with dilutions of up to 10(6) of Salmonella Typhimurium (ST) and Escherichia coli (EC). In method A, the dilution 10-5 of Salmonella Typhimurium corresponded to >57 MPN/mL and the dilution 10-6 was equal to 30 MPN/mL. There was a correlation between the counts used for the artificial contamination of the samples and those recovered by mMPN, indicating that the method A was sensitive for the enumeration of different levels of contamination of the meat matrix. In method B, there was no correlation between the inoculated dilutions and the mMPN results.(AU)

Isolamento de Salmonella Heidelberg em diferentes pontos da tecnologia de abate de frangos de corte / Salmonella Heidelberg isolated at different points of the broiler slaughterhouse

Bactérias do gênero Salmonella fazem parte da flora normal das aves e sua ocorrência em produtos avícolas varia com o manejo na criação e tecnologia de abate, representando riscos para o consumidor final e dificuldades nas exportações. A S. Heidelberg parece ser mais invasiva e causar doenças com maior gravidade que outros sorovares paratíficos. Este trabalho teve por objetivos pesquisar Salmonella em diferentes pontos da tecnologia de abate de frangos e relatar o isolamento de S. Heidelberg em um abatedouro sob inspeção federal. S. Heidelberg foi isolada em frangos logo após a depenagem e na água do chiller indicando que, embora não tenha sido realizado o isolamento em outros pontos amostrados, a bactéria estava presente no ambiente e poderia contaminar carcaças prontas para consumo, com reflexos na saúde pública. O isolamento de S. Heidelberg reforça esta preocupação uma vez que este sorovar tem se destacado como causador de doenças transmitidas por alimentos. (AU)

SALMONELLA HEIDELBERG ISOLATED AT DIFFERENT POINTS OF THE BROILER SLAUGHTERHOUSE. Bacteria of the genus Salmonella are part of the normal flora of poultry, and its occurrence in poultry products varies with the production management methods and slaughter technology, representing risks to the consumer and difficulties in exports. S. Heidelberg appears to be more invasive and to cause more severe disease than other non-typhoidal serovars. The objective of the present study was to search for Salmonella at different points of the slaughterhouse and to report on the isolation of S. Heidelberg in a slaughterhouse under federal inspection. S. Heidelberg was isolated in poultry soon after feathering-out and in the chiller water, indicating that, although it was not isolated at another sampled points, the bacteria was present in the environment and could contaminate carcasses ready for consumption, with an impact on public health. This concern is underscored by the fact that S. Heidelberg is a proven causative agent of foodborne infections. (AU)

Isolamento de Salmonella Heidelberg em diferentes pontos da tecnologia de abate de frangos de corte / Salmonella Heidelberg isolated at different points of the broiler slaughterhouse

Bactérias do gênero Salmonella fazem parte da flora normal das aves e sua ocorrência em produtos avícolas varia com o manejo na criação e tecnologia de abate, representando riscos para o consumidor final e dificuldades nas exportações. A S. Heidelberg parece ser mais invasiva e causar doenças com maior gravidade que outros sorovares paratíficos. Este trabalho teve por objetivos pesquisar Salmonella em diferentes pontos da tecnologia de abate de frangos e relatar o isolamento de S. Heidelberg em um abatedouro sob inspeção federal. S. Heidelberg foi isolada em frangos logo após a depenagem e na água do chiller indicando que, embora não tenha sido realizado o isolamento em outros pontos amostrados, a bactéria estava presente no ambiente e poderia contaminar carcaças prontas para consumo, com reflexos na saúde pública. O isolamento de S. Heidelberg reforça esta preocupação uma vez que este sorovar tem se destacado como causador de doenças transmitidas por alimentos.

SALMONELLA HEIDELBERG ISOLATED AT DIFFERENT POINTS OF THE BROILER SLAUGHTERHOUSE. Bacteria of the genus Salmonella are part of the normal flora of poultry, and its occurrence in poultry products varies with the production management methods and slaughter technology, representing risks to the consumer and difficulties in exports. S. Heidelberg appears to be more invasive and to cause more severe disease than other non-typhoidal serovars. The objective of the present study was to search for Salmonella at different points of the slaughterhouse and to report on the isolation of S. Heidelberg in a slaughterhouse under federal inspection. S. Heidelberg was isolated in poultry soon after feathering-out and in the chiller water, indicating that, although it was not isolated at another sampled points, the bacteria was present in the environment and could contaminate carcasses ready for consumption, with an impact on public health. This concern is underscored by the fact that S. Heidelberg is a proven causative agent of foodborne infections.

Isolamento de Salmonella Heidelberg em diferentes pontos da tecnologia de abate de frangos de corte

Bacteria of the genus Salmonella are part of the normal flora of poultry, and its occurrence in poultry products varies with the production management methods and slaughter technology, representing risks to the consumer and difficulties in exports. S. Heidelberg appears to be more invasive and to cause more severe disease than other non-typhoidal serovars. The objective of the present study was to search for Salmonella at different points of the slaughterhouse and to report on the isolation of S. Heidelberg in a slaughterhouse under federal inspection. S. Heidelberg was isolated in poultry soon after feathering-out and in the chiller water, indicating that, although it was not isolated at another sampled points, the bacteria was present in the environment and could contaminate carcasses ready for consumption, with an impact on public health. This concern is underscored by the fact that S. Heidelberg is a proven causative agent of foodborne infections.

Bactérias do gênero Salmonella fazem parte da flora normal das aves e sua ocorrência em produtos avícolas varia com o manejo na criação e tecnologia de abate, representando riscos para o consumidor final e dificuldades nas exportações. A S. Heidelberg parece ser mais invasiva e causar doenças com maior gravidade que outros sorovares paratíficos. Este trabalho teve por objetivos pesquisar Salmonella em diferentes pontos da tecnologia de abate de frangos e relatar o isolamento de S. Heidelberg em um abatedouro sob inspeção federal. S. Heidelberg foi isolada em frangos logo após a depenagem e na água do chiller indicando que, embora não tenha sido realizado o isolamento em outros pontos amostrados, a bactéria estava presente no ambiente e poderia contaminar carcaças prontas para consumo, com reflexos na saúde pública. O isolamento de S. Heidelberg reforça esta preocupação uma vez que este sorovar tem se destacado como causador de doenças transmitidas por alimentos.

Isolamento de Salmonella Heidelberg em diferentes pontos da tecnologia de abate de frangos de corte

Bacteria of the genus Salmonella are part of the normal flora of poultry, and its occurrence in poultry products varies with the production management methods and slaughter technology, representing risks to the consumer and difficulties in exports. S. Heidelberg appears to be more invasive and to cause more severe disease than other non-typhoidal serovars. The objective of the present study was to search for Salmonella at different points of the slaughterhouse and to report on the isolation of S. Heidelberg in a slaughterhouse under federal inspection. S. Heidelberg was isolated in poultry soon after feathering-out and in the chiller water, indicating that, although it was not isolated at another sampled points, the bacteria was present in the environment and could contaminate carcasses ready for consumption, with an impact on public health. This concern is underscored by the fact that S. Heidelberg is a proven causative agent of foodborne infections.

Bactérias do gênero Salmonella fazem parte da flora normal das aves e sua ocorrência em produtos avícolas varia com o manejo na criação e tecnologia de abate, representando riscos para o consumidor final e dificuldades nas exportações. A S. Heidelberg parece ser mais invasiva e causar doenças com maior gravidade que outros sorovares paratíficos. Este trabalho teve por objetivos pesquisar Salmonella em diferentes pontos da tecnologia de abate de frangos e relatar o isolamento de S. Heidelberg em um abatedouro sob inspeção federal. S. Heidelberg foi isolada em frangos logo após a depenagem e na água do chiller indicando que, embora não tenha sido realizado o isolamento em outros pontos amostrados, a bactéria estava presente no ambiente e poderia contaminar carcaças prontas para consumo, com reflexos na saúde pública. O isolamento de S. Heidelberg reforça esta preocupação uma vez que este sorovar tem se destacado como causador de doenças transmitidas por alimentos.

Sistema brasileiro de inspeção de produtos de origem animal - SISBI/POA - Nova oportunidade para inspeção e qualidade de produtos de origem animal / Brazilian System of Animal Origin Products Inspection – SISBI/POA – The new inspection opportunity and animal origin products quality

O Sistema Brasileiro de Inspeção de Produtos de Origem Animal – SISBI/POA é o novo sistema de inspe- ção integrante do SUASA, que tem como objetivo harmonizar e padronizar os procedimentos de inspe- ção e fiscalização dos produtos de origem animal em todo o país, possibilitando a ampliação do âmbito de comercialização dos produtos de origem animal nas diversas escalas de produção, preservando a inocuidade destes produtos e, conseqüentemente, a saúde pública, com o intuito de promover o desenvolvimento e a inclusão social de todas as regiões brasileiras. Este sistema é organizado e coordenado pelo Ministério da Agricultura, Pecuária e Abastecimento – MAPA, através da Divisão de Inspeção de Produtos de Origem Animal – DIPOA. A adesão ao sistema será voluntária, para os Estados, Distrito Federal e Municípios desde que atendam os princípios de equivalência preconizados na legislação.

The brazilian system of animal origin products inspection – SISBI/POA is the new inspection system of the SUASA, that has to harmonize and to standardize the inspection procedures and animal origin products fiscalization all over the country as purpose, making possible the animal origin products commercialization magnifying in the diverse production scales, preserving these products innocuity and, consequently the public health, with intention to promote the development and the social inclusion of all the brazilian regions. This system is organized and coordinated by the Ministry of Agriculture, Cattle and Supplying – MAPA, through the Inspection of Animal Origin products Division – DIPOA. The adhesion to the system will be voluntary to the states, federal district and cities since that they care about the praised principles equivalence in the legislation.

Sistema brasileiro de inspeção de produtos de origem animal - SISBI/POA - Nova oportunidade para inspeção e qualidade de produtos de origem animal / Brazilian System of Animal Origin Products Inspection SISBI/POA The new inspection opportunity and animal origin products quality

O Sistema Brasileiro de Inspeção de Produtos de Origem Animal SISBI/POA é o novo sistema de inspe- ção integrante do SUASA, que tem como objetivo harmonizar e padronizar os procedimentos de inspe- ção e fiscalização dos produtos de origem animal em todo o país, possibilitando a ampliação do âmbito de comercialização dos produtos de origem animal nas diversas escalas de produção, preservando a inocuidade destes produtos e, conseqüentemente, a saúde pública, com o intuito de promover o desenvolvimento e a inclusão social de todas as regiões brasileiras. Este sistema é organizado e coordenado pelo Ministério da Agricultura, Pecuária e Abastecimento MAPA, através da Divisão de Inspeção de Produtos de Origem Animal DIPOA. A adesão ao sistema será voluntária, para os Estados, Distrito Federal e Municípios desde que atendam os princípios de equivalência preconizados na legislação.(AU)

The brazilian system of animal origin products inspection SISBI/POA is the new inspection system of the SUASA, that has to harmonize and to standardize the inspection procedures and animal origin products fiscalization all over the country as purpose, making possible the animal origin products commercialization magnifying in the diverse production scales, preserving these products innocuity and, consequently the public health, with intention to promote the development and the social inclusion of all the brazilian regions. This system is organized and coordinated by the Ministry of Agriculture, Cattle and Supplying MAPA, through the Inspection of Animal Origin products Division DIPOA. The adhesion to the system will be voluntary to the states, federal district and cities since that they care about the praised principles equivalence in the legislation.(AU)