Resumo
Group B Streptococcus (GBS) is the most common cause of life-threatening infection in neonates. Guidelines from CDC recommend universal screening of pregnant women for rectovaginal GBS colonization. The objective of this study was to compare the performance of a combined enrichment/PCR based method targeting the atr gene in relation to culture using enrichment with selective broth medium (standard method) to identify the presence of GBS in pregnant women. Rectovaginal GBS samples from women at 36 weeks of pregnancy were obtained with a swab and analyzed by the two methods. A total of 89 samples were evaluated. The prevalence of positive results for GBS detection was considerable higher when assessed by the combined enrichment/PCR method than with the standard method (35.9% versus 22.5%, respectively). The results demonstrated that the use of selective enrichment broth followed by PCR targeting the atr gene is a highly sensitive, specific and accurate test for GBS screening in pregnant women, allowing the detection of the bacteria even in lightly colonized patients. This PCR methodology may provide a useful diagnostic tool for GBS detection and contributes for a more accurate and effective intrapartum antibiotic and lower newborn mortality and morbidity.
Resumo
The importance of table grape cultivation in São Paulo State, Brazil, is increasing in the last years, which is demonstrated by its acreage increase. Demands for technology improvements have followed, specially in respect to the development of new cultivars that can produce high quality grapes for exportation or internal markets purposes. Since the beginning of the grape breeding program at the Instituto Agronômico de Campinas (IAC) in 1943, no study was carried out on the parents used in crossings. In this study, hundreds of crossings, parents, as well as resulting progenies and their genealogical (pedigree) relationship were evaluated. According to the analysed data and parameters used we may consider as preferential the following cultivars: 'Itália', 'Patrícia', 'Soraya', 'Cardinal','Roberta', 'Maria Rosa', 'Carolina', 'Moscatel de Hamburgo', 'Lígia', 'Angelina', 'Marília', 'Alphonse Lavallée', 'Traviú', 'Ezequiel', 'Grão Mogol', 'Yole', 'Geni', 'A Dona', 'Aurora', 'Piratininga', 'Maria', 'Iracema'.
A cultura de uvas de mesa em São Paulo vem aumentando de importância, o que é flagrante pelos incrementos na área plantada verificados nos últimos anos. A demanda por tecnologia tem acompanhado esse crescimento, especialmente no que se refere a novas variedades que atendam tanto ao mercado externo, quanto ao interno, com uvas de alta qualidade. No programa de melhoramento para uvas de mesa no Instituto Agronômico, iniciado em 1943, praticamente nenhum estudo foi efetuado sobre os progenitores utilizados nos cruzamentos. Neste trabalho foram avaliados centenas de cruzamentos e as variedades envolvidas, bem como suas progênies e seu interrelacionamento genealógico. De acordo com os dados analisados e os parâmetros utilizados, foram considerados preferenciais num programa de cruzamento para uvas de mesa as seguintes variedades: 'Itália', 'Patrícia', 'Soraya', 'Cardinal', 'Roberta', 'Maria Rosa', 'Carolina', 'Moscatel de Hamburgo', 'Lígia', 'Angelina', 'Marília', 'Alphonse Lavallée', 'Traviú', 'Ezequiel', 'Grão Mogol', 'Yole', 'Geni', 'A Dona', 'Aurora', 'Piratininga', 'Maria', 'Iracema'.
Resumo
The importance of table grape cultivation in São Paulo State, Brazil, is increasing in the last years, which is demonstrated by its acreage increase. Demands for technology improvements have followed, specially in respect to the development of new cultivars that can produce high quality grapes for exportation or internal markets purposes. Since the beginning of the grape breeding program at the Instituto Agronômico de Campinas (IAC) in 1943, no study was carried out on the parents used in crossings. In this study, hundreds of crossings, parents, as well as resulting progenies and their genealogical (pedigree) relationship were evaluated. According to the analysed data and parameters used we may consider as preferential the following cultivars: 'Itália', 'Patrícia', 'Soraya', 'Cardinal','Roberta', 'Maria Rosa', 'Carolina', 'Moscatel de Hamburgo', 'Lígia', 'Angelina', 'Marília', 'Alphonse Lavallée', 'Traviú', 'Ezequiel', 'Grão Mogol', 'Yole', 'Geni', 'A Dona', 'Aurora', 'Piratininga', 'Maria', 'Iracema'.
A cultura de uvas de mesa em São Paulo vem aumentando de importância, o que é flagrante pelos incrementos na área plantada verificados nos últimos anos. A demanda por tecnologia tem acompanhado esse crescimento, especialmente no que se refere a novas variedades que atendam tanto ao mercado externo, quanto ao interno, com uvas de alta qualidade. No programa de melhoramento para uvas de mesa no Instituto Agronômico, iniciado em 1943, praticamente nenhum estudo foi efetuado sobre os progenitores utilizados nos cruzamentos. Neste trabalho foram avaliados centenas de cruzamentos e as variedades envolvidas, bem como suas progênies e seu interrelacionamento genealógico. De acordo com os dados analisados e os parâmetros utilizados, foram considerados preferenciais num programa de cruzamento para uvas de mesa as seguintes variedades: 'Itália', 'Patrícia', 'Soraya', 'Cardinal', 'Roberta', 'Maria Rosa', 'Carolina', 'Moscatel de Hamburgo', 'Lígia', 'Angelina', 'Marília', 'Alphonse Lavallée', 'Traviú', 'Ezequiel', 'Grão Mogol', 'Yole', 'Geni', 'A Dona', 'Aurora', 'Piratininga', 'Maria', 'Iracema'.