Efeito da melatonina na maturação in vitro de oócitos caprinos / Effect of melatonin on in vitro maturation of goat oocytes

To evaluate the effect of melatonin on in vitro maturation of goat oocytes, 12 females were used asoocyte donor. They were subjected to ovarian stimulation and ovum pickup by laparotomy. Oocytes wereevaluated and allocated into three groups of in vitro maturation: M0 (oocytes matured in TCM199 andsupplements); M10 and M50 (oocytes matured in M0 medium supplemented with 10 μM and 50 μM melatonin,respectively). All groups were subjected to the cell incubator for 24 hours at 38.5°C with a humidifiedatmosphere containing 5% CO2. For data analysis, Chi-square test was used, with a probability of 5% and acorrelation test. In conclusion, melatonin reduced in vitro maturation rates of goat oocytes.(AU)

Resposta folicular após estimulação ovariana com gonadotrofinas e polivinilpirrolidona em cabras Anglo Nubiana / Follicular response after ovarian stimulation with gonadotropins and polyvinylpyrrolidone in Anglo Nubian goats

To evaluate the effect of different hormonal stimulation treatments with PVP on ovarian folliculardevelopment, 27 goats received intravaginal devices (CIDR) for 10 days and 125 µg cloprostenol on day 8 oftreatment. The goats were distributed in the following groups: G1 (FSH-eCG), 300 IU of eCG and 70 mg pFSH,36 h prior to the withdrawal of CIDR; G2 (FSH), receiving 180 mg (40/40; 35/35, 30 mg) at 12 h intervals; G3(FSH-PVP10), receiving 70 mg FSH dissolved in PVP 10,000 MW, 24 hours before CIDR; and G4 (FSHPVP40),70 mg of FSH dissolved in PVP MW 40,000, 48 hours before CIDR. The follicles with diameter ≥ 2 mmwere counted, measured and classified. FSH-PVP 40 had a greater number of small follicles than FSH (P<0.05)and FSH showed greater number of large follicles (P < 0.05). In conclusion, treatment with FSH-PVP40 is analternative to stimulate ovaries in goats.(AU)

Efeito da melatonina na maturação in vitro de oócitos caprinos / Effect of melatonin on in vitro maturation of goat oocytes

To evaluate the effect of melatonin on in vitro maturation of goat oocytes, 12 females were used asoocyte donor. They were subjected to ovarian stimulation and ovum pickup by laparotomy. Oocytes wereevaluated and allocated into three groups of in vitro maturation: M0 (oocytes matured in TCM199 andsupplements); M10 and M50 (oocytes matured in M0 medium supplemented with 10 μM and 50 μM melatonin,respectively). All groups were subjected to the cell incubator for 24 hours at 38.5°C with a humidifiedatmosphere containing 5% CO2. For data analysis, Chi-square test was used, with a probability of 5% and acorrelation test. In conclusion, melatonin reduced in vitro maturation rates of goat oocytes.

Resposta folicular após estimulação ovariana com gonadotrofinas e polivinilpirrolidona em cabras Anglo Nubiana / Follicular response after ovarian stimulation with gonadotropins and polyvinylpyrrolidone in Anglo Nubian goats

To evaluate the effect of different hormonal stimulation treatments with PVP on ovarian folliculardevelopment, 27 goats received intravaginal devices (CIDR) for 10 days and 125 µg cloprostenol on day 8 oftreatment. The goats were distributed in the following groups: G1 (FSH-eCG), 300 IU of eCG and 70 mg pFSH,36 h prior to the withdrawal of CIDR; G2 (FSH), receiving 180 mg (40/40; 35/35, 30 mg) at 12 h intervals; G3(FSH-PVP10), receiving 70 mg FSH dissolved in PVP 10,000 MW, 24 hours before CIDR; and G4 (FSHPVP40),70 mg of FSH dissolved in PVP MW 40,000, 48 hours before CIDR. The follicles with diameter ≥ 2 mmwere counted, measured and classified. FSH-PVP 40 had a greater number of small follicles than FSH (P<0.05)and FSH showed greater number of large follicles (P < 0.05). In conclusion, treatment with FSH-PVP40 is analternative to stimulate ovaries in goats.