Carotenoid production in sugarcane juice and synthetic media supplemented with nutrients by Rhodotorula rubra l02

In order for the use of biological carotenoids to become feasible, it is necessary to have adequate low cost sources and improved methods of cultivation. The aim of this study was to evaluate the effect of supplementation with nitrogen, phosphorus, zinc, and magnesium, on the biomass and carotenoid volumetric production by yeast Rhodotorula rubra L02 using a complex medium (sugarcane juice) and synthetic media (sucrose and maltose) as substrates. The experimental design used for each substrate was randomized in blocks with 16 treatments and 3 repetitions. The treatments were compound for 15 different combinations of nutrients (N; Mg; Zn; P, N + Mg; N + Zn; N + P; Mg + Zn; Mg + P; Zn + P; N + P + Zn; N + P + Mg; N + Zn + Mg; P + Zn + Mg; N + Zn + Mg + P) alone and combined, and a control. The results were submitted to analysis of variance and Tukey test at 5% significance level. Among the treatments evaluated, the highest production of dry biomass, with both maltose and sucrose, was observed for Mg (1.60 g/L and 1.94 g/L, respectively). Additionally, another treatment that stood out in terms of biomass production was the control treatment with maltose (1.54 g/L). After the incubation time, killer activity was not observed since there was no formation of inhibition halo around the L02 yeast.(AU)

Produção de bioetanol a partir da fermentação de caldo de sorgo sacarino e cana-de-açúcar / Bioethanol production from sweet sorghum broth and sugar cane fermentation

Os biocombustíveis apresentam-se com grande importância para suprir a demanda global de energia. São produzidos a partir de biomassa vegetal, emitem menor quantidade de dióxido de carbono e de partículas poluentes ao ambiente quando utilizados e possuem grande vantagem por serem combustíveis renováveis. Entre as matérias-primas com potencial para produção de etanol, cita-se o sorgo sacarino. Objetivou-se comparar o processamento industrial do genótipo de sorgo sacarino CVSW80007 e da cultivar de cana-de-açúcar 'RB966928' para produção de bioetanol em início de safra. As análises realizadas foram: brix; pH, ART, AR, acidez total, ARRT, glicerol, teor alcoolico, viabilidade celular, viabilidade de brotos e brotamentos. Quanto às características químico-tecnológicas, as matérias-primas apresentaram-se aptas ao processamento industrial, com índices superiores para a cana-de-açúcar. O desenvolvimento das fermentações ocorreu de forma adequada para ambas, sendo que o mosto fermentado (vinho), produzido a partir do mosto de cana-de-açúcar, apresentou maior teor alcoolico e rendimento fermentativo.(AU)

Biofuels have great importance to supply the global energy demand. These fuels are produced from plant biomass, emit less carbon dioxide and particulate pollutants to the environment when used and have great advantage of being renewable fuels. Among the raw materials with potential for ethanol production, is cited sweet sorghum. This study aimed to compare industrial processing of genotype sorghum CVSW80007 and the cultivar sugar cane 'RB966928' for bioethanol production in early season. The analyzes performed were: brix, pH, ART, AR, total acidity, ARRT, glycerol, alcohol content, cell viability, shoots viability and buds. Regarding the chemical-technological characteristics, raw materials were suitable for industrial processing, with higher rates for sugar cane. The development of fermentations occurred appropriately for both, and the fermented must (wine) produced from the must of sugar cane had higher alcohol content and fermentation yield.(AU)

Avaliação tecnológica e microbiológica da fermentação etanólica de caldo de sorgo sacarino

O aumento da demanda por combustíveis tem intensificado a busca por fontes renováveis de energia principalmente as biomassas, destacando-se a canade- açúcar na produção de bioetanol. Além desta a cultura do sorgo sacarino apresenta elevado potencial principalmente por utilizar o mesmo complexo industrial de produção e ser processado no período de entressafra da cana. Objetivou-se com a presente pesquisa avaliar o potencial de produtividade, características químicotecnológicas e processo fermentativo de três genótipos de sorgo utilizado como matéria-prima complementar para produção de etanol, no período de início de safra. O delineamento experimental foi o em Blocos ao Acaso num modelo Fatorial 3x7, com três repetições, para Avaliações Biométricas, sendo que para as Características Químico?tecnológicas e Processo Fermentativo (para cada uma das leveduras estudadas) utilizou-se o modelo 3x2x6. Avaliou-se a Altura (m) das plantas, Diâmetro da base, Diâmetro da ponta, número de folhas, comprimento da panícula (cm), biomassa total (t/ha), colmos limpos (t/ha) e % de colmos, nas analises biométricas; o teor de sólidos solúveis (Brix), Açúcares Redutores Totais (ART), pH, Acidez Total, Compostos Fenólicos Totais e Teor de Amido do caldo extraído nas analises químico-tecnológicas e a viabilidade celular, viabilidade de brotos e brotamento no processo fermentativo. Os resultados obtidos indicam que os genótipos CVSW80007 e CVWS80147 apresentam melhores características químico-tecnológicas...

The increased in demand for combustible has intensified the search for renewable energies mainly from biomass, especially the sugarcane for bioethanol production. Besides of this crop, sweet sorghum has shown great potential in the production of biofuels, mainly because it allows the use of the same industrial park production of sugarcane, and can be processed in the off-season of this crop. The objective of this research was to evaluate the potential of three genotypes of sorghum harvested with and without leaves on the productivity, technologicalchemical characteristics, and ethanol production in laboratorial scale using two yeast strains, from the beginning of the sugarcane harvest. The experimental design was randomized blocks in a 3x7 factorial design, with three replicates for biometric analysis. For chemical and technological characteristics and fermentation process (for each of the yeasts studied) was used 3x2x6 model. Was evaluated height (m) of plant, base and tip stalk diameter, leaf number, panicle length (cm), total biomass yield (t/ha), clean stalk yield (t/ha) and percentage stem, in biometrics analyses. Soluble solids content (Brix), total reducing sugars (TRS), pH, total acidity, total phenolic compounds and starch content of the juice extracted in chemicaltechnological analyses. Was evaluated the cell viability, viability and budding of the yeasts in the fermentation process. Was evaluated the cell viability, viability of sprouts and budding of the yeasts in the fermentation process. Was determined the ethanol production (L/ha and L/t) in laboratory scale. The results indicate that the genotypes CVSW80007 and CVWS80147 have better chemical and technological characteristics, with useful industrialization period (UIP) of 45 days...