Enhancement of extender excellence of frozen bull semen using alfa-tocopherol as an antioxidant

Background: Addition of the antioxidant to extender media is the most appropriate attempt to reduce structural lossesencounter during the process of cryopreservation. Hence semen excellence could be maintained for longer duration withoutadverse impact. Additionally antioxidants are not only capturing the reactive oxygen species but also improve the spermquality indicators and fertility. Accordingly, current elucidation has been executed to explore the dose depended appraisalof varied concentration of α-tocopherol in Tris-based extender on frozen-thawed bull semen quality parameters for enhancement of bull semen cryopreservation in the subtropical ecosystem of Peshawar.Materials, Methods & Results: Experiments were carried out on semen that has been collected from both Achai-an indigenous breed and Holstein Friesian (HF) - the exotic breed in artificial vagina maintained at 42°C from the experimentalbulls of either breed and processed independently breed wise. Semen specimens with above 70% motility were evaluatedseparately breed-wise under same environmental condition. Standard procedure was adopted to extend the collected semenin the experimental extenders and frozen subsequently. After thawing, further Analysis of the frozen straws of semen wascarried out for sperm excellence indicators that include motility, viability, acrosomal integrity and functional integrity ofspermatozoa under the subtropical condition. Sperm viability and acrosomal integrity were determined by dual stainingprocedure i.e. Trypan-blue and Giemsa stains. The hypo-osmotic swelling (HOS) test was used to assess plasma membraneintegrity. The current elucidation demonstrated that α-tocopherol 1.5 mg/mL supplemented in extender had significantly(P < 0.05) increased sperm excellence gauge that includes motilityy, viability, acrosomal integrity and functional membraneintegrity in both the breeds. On the other hand, the result further elucidated...(AU)

Enhancement of extender excellence of frozen bull semen using alfa-tocopherol as an antioxidant

Background: Addition of the antioxidant to extender media is the most appropriate attempt to reduce structural lossesencounter during the process of cryopreservation. Hence semen excellence could be maintained for longer duration withoutadverse impact. Additionally antioxidants are not only capturing the reactive oxygen species but also improve the spermquality indicators and fertility. Accordingly, current elucidation has been executed to explore the dose depended appraisalof varied concentration of α-tocopherol in Tris-based extender on frozen-thawed bull semen quality parameters for enhancement of bull semen cryopreservation in the subtropical ecosystem of Peshawar.Materials, Methods & Results: Experiments were carried out on semen that has been collected from both Achai-an indigenous breed and Holstein Friesian (HF) - the exotic breed in artificial vagina maintained at 42°C from the experimentalbulls of either breed and processed independently breed wise. Semen specimens with above 70% motility were evaluatedseparately breed-wise under same environmental condition. Standard procedure was adopted to extend the collected semenin the experimental extenders and frozen subsequently. After thawing, further Analysis of the frozen straws of semen wascarried out for sperm excellence indicators that include motility, viability, acrosomal integrity and functional integrity ofspermatozoa under the subtropical condition. Sperm viability and acrosomal integrity were determined by dual stainingprocedure i.e. Trypan-blue and Giemsa stains. The hypo-osmotic swelling (HOS) test was used to assess plasma membraneintegrity. The current elucidation demonstrated that α-tocopherol 1.5 mg/mL supplemented in extender had significantly(P < 0.05) increased sperm excellence gauge that includes motilityy, viability, acrosomal integrity and functional membraneintegrity in both the breeds. On the other hand, the result further elucidated...

A study on vas deference and seminal vesicle of male goats (Capra hircus)

Background: Reproductive activity of male animal is strongly associated with development of the testicle, the conductor channels and accessory sex gland. Vas deference may be considered the extra-testicular continuation from cauda of the epididymis, and it is the portion of the reproductive tract fundamentally associated with transportation of the sperm-containing fluid from each epididymis to the urethra for their finishing discharge. Non-ampulated part is further divided into: extra-abdominal portion along the caudal border of testes, up to the vaginal ring and abdominal portion started from the vaginal ring to ampullae. Accessory sex glands including vesicular gland and ampullae of vas deferens are characteristically essential for reproductive process. Gross morphometric features of anatomical structures of male reproductive tract in an animal make available a very valuable mechanism in understanding of several physiological and reproductive phenomena. The current study aim at documenting baseline data on gross morphometric aspects of vas deference and seminal vesicle in adult male local nondescript goats in Pakistan ecology that could be utilized as reference values in evaluating their congenital defects and gross pathological abnormalities.Materials, Methods & Results: Experiments were carried out on ampullated and non-ampullated segment of vas deference and seminal vesicle. A total of n = 100 local male goats of 2-3 years of age were selected for this study. Vas deference and seminal vesicles (accessory sex gland) were collected immediately after slaughter from local abattoirs. The non-ampullated segment of vas deference was further divided into abdominal and extra-abdominal segment. Specimens were dissected and washed with normal saline. Standard procedure was adopted for these morphometric features of vas deference and seminal vesicle by using vernier caliper, scale, non-stretchable thread and electronic weighing balance.[...](AU)

A study on vas deference and seminal vesicle of male goats (Capra hircus)

Background: Reproductive activity of male animal is strongly associated with development of the testicle, the conductor channels and accessory sex gland. Vas deference may be considered the extra-testicular continuation from cauda of the epididymis, and it is the portion of the reproductive tract fundamentally associated with transportation of the sperm-containing fluid from each epididymis to the urethra for their finishing discharge. Non-ampulated part is further divided into: extra-abdominal portion along the caudal border of testes, up to the vaginal ring and abdominal portion started from the vaginal ring to ampullae. Accessory sex glands including vesicular gland and ampullae of vas deferens are characteristically essential for reproductive process. Gross morphometric features of anatomical structures of male reproductive tract in an animal make available a very valuable mechanism in understanding of several physiological and reproductive phenomena. The current study aim at documenting baseline data on gross morphometric aspects of vas deference and seminal vesicle in adult male local nondescript goats in Pakistan ecology that could be utilized as reference values in evaluating their congenital defects and gross pathological abnormalities.Materials, Methods & Results: Experiments were carried out on ampullated and non-ampullated segment of vas deference and seminal vesicle. A total of n = 100 local male goats of 2-3 years of age were selected for this study. Vas deference and seminal vesicles (accessory sex gland) were collected immediately after slaughter from local abattoirs. The non-ampullated segment of vas deference was further divided into abdominal and extra-abdominal segment. Specimens were dissected and washed with normal saline. Standard procedure was adopted for these morphometric features of vas deference and seminal vesicle by using vernier caliper, scale, non-stretchable thread and electronic weighing balance.[...]