Rangelia vitalii, Babesia spp. and Ehrlichia spp. in dogs in Passo Fundo, state of Rio Grande do Sul, Brazil / Rangelia vitalii, Babesia spp. e Ehrlichia spp. em cães de Passo Fundo, estado do Rio Grande do Sul, Brasil

Pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. Fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents Babesia vogeli and Ehrlichia canis and conventional PCR for Babesia spp. (gene 18S rRNA), Rangelia vitalii (gene 18S rRNA) and Ehrlichia spp. (gene dsb). Five (8.6%) of the 58 dogs were serologically positive for Babesia spp. and three (5.1%) for E. canis. Four dogs (6.8%) were positive for R. vitalii through the molecular diagnosis. The PCR products were sequenced and the DNA from R. vitalii was found to be 99% genetically identical to samples of R. vitalii that had been isolated in Brazil. No presence of Babesia spp. or E. canis was observed through PCR on the dogs evaluated here. The results indicate the presence of R. vitalii and exposure to Babesia spp. and Ehrlichia spp. among the dogs analyzed.(AU)

Patógenos transmitidos por carrapatos são um problema emergente em todo o mundo, o trabalho objetivou diagnosticar os agentes causais da infecção em cães com suspeita de hemoparasitoses. Cinquenta e oito caninos com sinais clínicos como depressão, diáteses hemorrágicas e febre foram avaliados quanto à apresentação clínica, hemograma, esfregaço sanguíneo, sorologia pelo método de Imunofluorescência Indireta para os agentes Babesia vogeli e Ehrlichia canis e na PCR convencional para Babesia spp. (gene 18S rRNA), Rangelia vitalii (gene 18S rRNA) e Ehrlichia spp. (gene dsb). Cinco (8,6%) dos 58 cães apresentaram sorologia positiva para Babesia spp. e três (5,1%) para E. canis. Quatro (6,8%) animais mostraram-se positivos para R. vitalii no diagnóstico molecular. Os produtos da PCR foram sequenciados e o DNA encontrado de R. vitalii mostrou 99% de identidade genética com amostras de R. vitalii isoladas no Brasil. Não foi observada a presença de Babesia spp. e E. canis na PCR dos cães avaliados. Os resultados indicaram a presença de R. vitalii e exposição a Babesia spp. e Ehrlichia spp. entre os cães analisados.(AU)

Traumatic Panniculitis in a dog / Paniculite traumática em um cão

Background: Panniculitis can be associated with a group of multifactorial diseases that develop intense inflammatory response of the subcutaneous fat tissue. The damaged fat cells suffer hydrolysis and saponification. The etiology remains unknown, although it has been associated with infectious agents, vascular pathologies, pancreatic disorders, neoplasms, immunologic diseases and nutritional deficiencies. Nodular sterile panniculitis is referred to the fat subcutaneous tissue inflammation without the presence of microorganisms and generally is presented with multiple or single nodules associated either with systemic or traumatic issues. This assay reports a case of traumatic Panniculitis presented as a single nodule on a dog admitted at the Veterinary Teaching Hospital of Passo Fundo University (HV-UPF), State of Rio Grande do Sul, Brazil, emphasizing the clinical signs, diagnosis and surgical treatment.Case: An adult mixed breed bitch, weighting 7,5 kg, was admitted at the HV-UPF because of a one-month evolution of a dorsal thoracic subcutaneous mass. Symptomatic treatment with unknown oral antibiotics was administered by another veterinarian, who also tried to perform local puncture drainage unsuccessfully. There was no previous history of local puncture or subcutaneous drug infiltration that could explain the mass. During physical examination, a single, painless and nodular

Background: Panniculitis can be associated with a group of multifactorial diseases that develop intense inflammatory response of the subcutaneous fat tissue. The damaged fat cells suffer hydrolysis and saponification. The etiology remains unknown, although it has been associated with infectious agents, vascular pathologies, pancreatic disorders, neoplasms, immunologic diseases and nutritional deficiencies. Nodular sterile panniculitis is referred to the fat subcutaneous tissue inflammation without the presence of microorganisms and generally is presented with multiple or single nodules associated either with systemic or traumatic issues. This assay reports a case of traumatic Panniculitis presented as a single nodule on a dog admitted at the Veterinary Teaching Hospital of Passo Fundo University (HV-UPF), State of Rio Grande do Sul, Brazil, emphasizing the clinical signs, diagnosis and surgical treatment.Case: An adult mixed breed bitch, weighting 7,5 kg, was admitted at the HV-UPF because of a one-month evolution of a dorsal thoracic subcutaneous mass. Symptomatic treatment with unknown oral antibiotics was administered by another veterinarian, who also tried to perform local puncture drainage unsuccessfully. There was no previous history of local puncture or subcutaneous drug infiltration that could explain the mass. During physical examination, a single, painless and nodular

Paniculite traumática em um cão / Traumatic panniculitis in a dog

Background: Panniculitis can be associated with a group of multifactorial diseases that develop intense inflammatory response of the subcutaneous fat tissue. The damaged fat cells suffer hydrolysis and saponification. The etiology remains unknown, although it has been associated with infectious agents, vascular pathologies, pancreatic disorders, neoplasms, immunologic diseases and nutritional deficiencies. Nodular sterile panniculitis is referred to the fat subcutaneous tissue inflammation without the presence of microorganisms and generally is presented with multiple or single nodules associated either with systemic or traumatic issues. This assay reports a case of traumatic Panniculitis presented as a single nodule on a dog admitted at the Veterinary Teaching Hospital of Passo Fundo University (HV-UPF), State of Rio Grande do Sul, Brazil, emphasizing the clinical signs, diagnosis and surgical treatment. Case: An adult mixed breed bitch, weighting 7,5 kg, was admitted at the HV-UPF because of a one-month evolution of a dorsal thoracic subcutaneous mass. Symptomatic treatment with unknown oral antibiotics was administered by another veterinarian, who also tried to perform local puncture drainage unsuccessfully. There was no previous history of local puncture or subcutaneous drug infiltration that could explain the mass. During physical examination, a single, painless and nodular subcutaneous mass (4 x 6 cm) was located on the dorsal thoracic median line, below the scapula, discharging a serosanguineous fluid probably due to the previous puncture. No other occurrences were found. The patient was treated with cephalexin and meloxicam. Hematological and biochemistry examinations were within reference range. Cytological exam demonstrated slightly degenerated neutrophilis and a great amount of adipose tissue. This could suggest, among other pathologies, neoplasm. Surgery was performed after 15 days. An elliptical incision was performed maintaining a 2,5 cm safety margin from the mass. Histopathological findings were compatible with sterile nodular panniculitis, likely traumatic. Discussion: There was a single, firm, regular, painless nodule, correspondent with other studies. The lesion location was also similar to other author's findings. Cytological findings were unspecific and could lead to several diagnoses. Careful pathologic study is necessary to differentiate from benign or malignant neoplasms and other affections. The hematological and biochemistry examinations, ultrasounds and radiographs were all within reference range on the case. These diagnose tools are very important and should always be used in order to achieve the correct screening, diagnosis and treatment. Surgical removal was considered effective, once 7 months after the treatment there was no local recurrence, with great cosmetic result.

Cistolitectomia videolaparoscópica para a remoção de múltiplos urólitos em cão pinscher obeso Relato de caso / Videolaparoscopic cystolitectomy for multiples urolith removal in obese pinscherdog Case report

A cistotomia é o procedimento mais comumente realizado no trato urinário de animais de companhiapara a remoção de cálculos vesicais, podendo ser executada por diferentes acessos. Considerando osnumerosos benefícios que a cirurgia laparoscópica apresenta em relação à cirurgia convencional, opresente relato descreve o caso de um canino fêmea, Pinscher, de 5,1kg que apresentava múltiploscálculos vesicais, no qual se realizou o diagnóstico, e o posterior tratamento, através de cistolitectomiavideolaparoscópica. Para o procedimento cirúrgico foram utilizados três portais (dois de 10mme um de 5mm) dispostos na linha média ventral e nas paredes abdominais direita e esquerda. Apósa remoção dos urólitos, obteve-se a cistorrafia por meio de sutura intracorpórea em duas camadas, aprimeira em padrão contínuo simples, e a segunda em Lembert contínuo. As litíases foram retiradasda cavidade abdominal através do acesso do segundo portal com o auxílio de um dedo de luva. Nãoocorreram complicações trans ou pós-operatórias. O presente relato demonstra que a cirurgia laparoscópicapode ser utilizada com sucesso na terapêutica de múltiplos cálculos vesicais em cães depequeno porte e obesos.(AU)

Cystotomy is the most commonly procedure performed in the urinary tract of small animals for theremoval of bladder stones, which can be performed by different approaches. Considering the numerouslaparoscopic surgery benefits, this report describes the case of a eight-years-old female DobermanPinscher, weighing 5.1 kg with multiple bladder stones. The diagnosis and subsequent treatmentwere done by laparoscopic cystolitectomy. For the surgical procedure was used three portals (two10mm and one 5mm) localized in the ventral midline and the right and left abdominal wall. After theuroliths removal, the cistorrafy was done in two layers. The first one was done by simple continuouspattern, and in the second in continuous Lembert. The urolithis were removed from the abdominal cavity by the second portal access using a glove finger. The postoperative period was uneventful. Thisreport demonstrates that the laparoscopic surgery can be successfully used in the treatment of multiplebladder stones in small obese dogs.(AU)

Cistolitectomia videolaparoscópica para a remoção de múltiplos urólitos em cão pinscher obeso – Relato de caso / Videolaparoscopic cystolitectomy for multiples urolith removal in obese pinscherdog – Case report

A cistotomia é o procedimento mais comumente realizado no trato urinário de animais de companhiapara a remoção de cálculos vesicais, podendo ser executada por diferentes acessos. Considerando osnumerosos benefícios que a cirurgia laparoscópica apresenta em relação à cirurgia convencional, opresente relato descreve o caso de um canino fêmea, Pinscher, de 5,1kg que apresentava múltiploscálculos vesicais, no qual se realizou o diagnóstico, e o posterior tratamento, através de cistolitectomiavideolaparoscópica. Para o procedimento cirúrgico foram utilizados três portais (dois de 10mme um de 5mm) dispostos na linha média ventral e nas paredes abdominais direita e esquerda. Apósa remoção dos urólitos, obteve-se a cistorrafia por meio de sutura intracorpórea em duas camadas, aprimeira em padrão contínuo simples, e a segunda em Lembert contínuo. As litíases foram retiradasda cavidade abdominal através do acesso do segundo portal com o auxílio de um dedo de luva. Nãoocorreram complicações trans ou pós-operatórias. O presente relato demonstra que a cirurgia laparoscópicapode ser utilizada com sucesso na terapêutica de múltiplos cálculos vesicais em cães depequeno porte e obesos.

Cystotomy is the most commonly procedure performed in the urinary tract of small animals for theremoval of bladder stones, which can be performed by different approaches. Considering the numerouslaparoscopic surgery benefits’, this report describes the case of a eight-years-old female DobermanPinscher, weighing 5.1 kg with multiple bladder stones. The diagnosis and subsequent treatmentwere done by laparoscopic cystolitectomy. For the surgical procedure was used three portals (two10mm and one 5mm) localized in the ventral midline and the right and left abdominal wall. After theuroliths removal, the cistorrafy was done in two layers. The first one was done by simple continuouspattern, and in the second in continuous Lembert. The urolithis were removed from the abdominal cavity by the second portal access using a glove finger. The postoperative period was uneventful. Thisreport demonstrates that the laparoscopic surgery can be successfully used in the treatment of multiplebladder stones in small obese dogs.

Traumatic Panniculitis in a dog / Paniculite traumática em um cão

Background: Panniculitis can be associated with a group of multifactorial diseases that develop intense inflammatory response of the subcutaneous fat tissue. The damaged fat cells suffer hydrolysis and saponification. The etiology remains unknown, although it has been associated with infectious agents, vascular pathologies, pancreatic disorders, neoplasms, immunologic diseases and nutritional deficiencies. Nodular sterile panniculitis is referred to the fat subcutaneous tissue inflammation without the presence of microorganisms and generally is presented with multiple or single nodules associated either with systemic or traumatic issues. This assay reports a case of traumatic Panniculitis presented as a single nodule on a dog admitted at the Veterinary Teaching Hospital of Passo Fundo University (HV-UPF), State of Rio Grande do Sul, Brazil, emphasizing the clinical signs, diagnosis and surgical treatment.Case: An adult mixed breed bitch, weighting 7,5 kg, was admitted at the HV-UPF because of a one-month evolution of a dorsal thoracic subcutaneous mass. Symptomatic treatment with unknown oral antibiotics was administered by another veterinarian, who also tried to perform local puncture drainage unsuccessfully. There was no previous history of local puncture or subcutaneous drug infiltration that could explain the mass. During physical examination, a single, painless and nodular

Background: Panniculitis can be associated with a group of multifactorial diseases that develop intense inflammatory response of the subcutaneous fat tissue. The damaged fat cells suffer hydrolysis and saponification. The etiology remains unknown, although it has been associated with infectious agents, vascular pathologies, pancreatic disorders, neoplasms, immunologic diseases and nutritional deficiencies. Nodular sterile panniculitis is referred to the fat subcutaneous tissue inflammation without the presence of microorganisms and generally is presented with multiple or single nodules associated either with systemic or traumatic issues. This assay reports a case of traumatic Panniculitis presented as a single nodule on a dog admitted at the Veterinary Teaching Hospital of Passo Fundo University (HV-UPF), State of Rio Grande do Sul, Brazil, emphasizing the clinical signs, diagnosis and surgical treatment.Case: An adult mixed breed bitch, weighting 7,5 kg, was admitted at the HV-UPF because of a one-month evolution of a dorsal thoracic subcutaneous mass. Symptomatic treatment with unknown oral antibiotics was administered by another veterinarian, who also tried to perform local puncture drainage unsuccessfully. There was no previous history of local puncture or subcutaneous drug infiltration that could explain the mass. During physical examination, a single, painless and nodular