Resumo
Bovine clinical mastitis caused by Staphylococcus spp. is a serious and widespread disease in the world of dairy farming. Antimicrobial therapy is of fundamental importance in the prevention and treatment of infectious mastitis, but the indiscriminate use of antimicrobials acts as a determining factor for the spread of the disease. The present study evaluated the resistance profiles of 57 Staphylococcus spp. isolated from bovine clinical mastitis to beta-lactams and gentamicin, relating characteristics of phenotype (in vitro susceptibility tests) and genotype (detection and expression of genes encoding resistance - mecA, mecALGA251, blaZ, femA, femB, and aacA-aphD - using PCR and RT-PCR, respectively). One or more genes coding for resistance to different antimicrobials were detected in 50 Staphylococcus spp. isolates. The femA and femB genes were the most frequent (75.4% for both). The observed expression of the genes was as follows: blaZ (60%), femA (39.5%), aacA-aphD (50%), femB (32.6%), mecA (8.3%), and mecALGA251 (0%). Considering the relevance of the genus Staphylococcus to bovine mastitis, this study aimed to elucidate aspects regarding the genotypic and phenotypic profiles of these microorganisms so as to contribute to the development of effective strategies for mastitis control.(AU)
A mastite clínica bovina causada por Staphylococcus spp. é uma doença grave e generalizada no mundo da pecuária leiteira. A terapia antimicrobiana é de fundamental importância na prevenção e no tratamento da mastite infecciosa, mas o uso indiscriminado de antimicrobianos atua como fator determinante para a disseminação da doença. O presente estudo avaliou os perfis de resistência de 57 Staphylococcus spp. isolados de mastite clínica bovina em relação ao uso de betalactâmicos e gentamicina, relacionando características do fenótipo (testes de suscetibilidade in vitro) e genótipo (detecção e expressão de genes que codificam resistência - mecA, mecALGA251, blaZ, femA, femB, e aacA-aphD - usando PCR e RT-PCR, respectivamente). Um ou mais genes que codificam resistência a diferentes antimicrobianos foram detectados em 50 Staphylococcus spp. isolados. Os genes femA e femB foram os mais frequentes (75,4% para ambos). A expressão observada dos genes foi a seguinte: blaZ (60%), femA (39,5%), aacA-aphD (50%), femB (32,6%), mecA (8,3%) e mecALGA251 (0%). Considerando-se a relevância do gênero Staphylococcus para a mastite bovina, este estudo teve como objetivo elucidar aspectos referentes aos perfis genotípico e fenotípico desses microrganismos, a fim de contribuir para o desenvolvimento de estratégias eficazes para o controle da mastite.(AU)
Assuntos
Staphylococcus/isolamento & purificação , Expressão Gênica/genética , Resistência beta-Lactâmica/genética , Farmacorresistência Bacteriana/genética , Mastite Bovina/microbiologia , Gentamicinas , Reação em Cadeia da Polimerase Via Transcriptase ReversaResumo
With the raise of SARS-CoV-2 in late 2019 and the associated COVID-19 pandemics, much of the knowledge gathered from decades on research on other coronavirus species that use humans and other animals as hosts is invaluable to help counteract the burden of this new disease. This review ais to bring the highlights on coronavirus Biology and the most frequent diseases they might cause, serving rather as an introduction to the field.
Assuntos
Coronavirus , Saúde Pública , Coronavírus Relacionado à Síndrome Respiratória Aguda GraveResumo
With the raise of SARS-CoV-2 in late 2019 and the associated COVID-19 pandemics, much of the knowledge gathered from decades on research on other coronavirus species that use humans and other animals as hosts is invaluable to help counteract the burden of this new disease. This review ais to bring the highlights on coronavirus Biology and the most frequent diseases they might cause, serving rather as an introduction to the field.(AU)
Assuntos
Coronavirus , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave , Saúde PúblicaResumo
With the raise of SARS-CoV-2 in late 2019 and the associated COVID-19 pandemics, much of the knowledge gathered from decades on research on other coronavirus species that use humans and other animals as hosts is invaluable to help counteract the burden of this new disease. This review ais to bring the highlights on coronavirus Biology and the most frequent diseases they might cause, serving rather as an introduction to the field.