Adverse reactions to oil-emulsified (ISA-70) Newcastle disease vaccine in a pigeon flock

Oil adjuvants in combination with inactivated vaccines against several avian diseases are widely used. In spite of some advantages of oil adjuvants, a local tissue reaction has been reported in some animals. In this report, about 30 birds of a flock of pigeons with 40 birds, developed scattered small to large nodules about 5 mm to 2 cm in diameter in subcutaneous tissues and pectoral muscles after intramuscularly injection of an oil-emulsified (ISA-70) Newcastle disease vaccine. Histopathologic examination showed granulomatous and cystic reactions in the site of injection. Bacteriological examinations as well as fresh smears stained with Ziehl-Neelsen, Brown-Brenn Gram and PAS stains were void of bacteria or fungi. It may be prudent to consider the potential for induction of adverse reaction of vaccine formulations containing mineral oils as adjuvant in pigeons.

Adverse reactions to oil-emulsified (ISA-70) Newcastle disease vaccine in a pigeon flock

Oil adjuvants in combination with inactivated vaccines against several avian diseases are widely used. In spite of some advantages of oil adjuvants, a local tissue reaction has been reported in some animals. In this report, about 30 birds of a flock of pigeons with 40 birds, developed scattered small to large nodules about 5 mm to 2 cm in diameter in subcutaneous tissues and pectoral muscles after intramuscularly injection of an oil-emulsified (ISA-70) Newcastle disease vaccine. Histopathologic examination showed granulomatous and cystic reactions in the site of injection. Bacteriological examinations as well as fresh smears stained with Ziehl-Neelsen, Brown-Brenn Gram and PAS stains were void of bacteria or fungi. It may be prudent to consider the potential for induction of adverse reaction of vaccine formulations containing mineral oils as adjuvant in pigeons.(AU)

Immunohistochemical and Morphopathological Features of Multiple Cutaneous Mast Cell Tumor in a Cow

A 4 year old female Holstein cow with multiple skin lesions was referred for clinical examination. Grossly, approximately 60 discrete cutaneous nodules and masses with variable sizes ranging from 1 to 5 cm were observed on both sides of flunks, shoulders, neck, back, and mammary glands. Histopathologically, the masses were composed of non-encapsulated accumulation of neoplastic round cells contained moderate to abundant amounts of finely eosinophilic granules in the cytoplasm. Cellular or nuclear pleomorphism and mitotic figures were not remarkable. Metachromasia was seen in cytoplasmic granules stained with toluidine blue. In the immunohistochemical staining, the neoplastic cells were positive for vimentin and c-kit. No immunoreactivity was seen for cytokeratin and HMB - 45. Based on these findings, multiple cutaneous mast cell tumor was diagnosed. Immunohistochemical features of mast cell tumor have not been reported previously in cattle.

Immunohistochemical and Morphopathological Features of Multiple Cutaneous Mast Cell Tumor in a Cow

A 4 year old female Holstein cow with multiple skin lesions was referred for clinical examination. Grossly, approximately 60 discrete cutaneous nodules and masses with variable sizes ranging from 1 to 5 cm were observed on both sides of flunks, shoulders, neck, back, and mammary glands. Histopathologically, the masses were composed of non-encapsulated accumulation of neoplastic round cells contained moderate to abundant amounts of finely eosinophilic granules in the cytoplasm. Cellular or nuclear pleomorphism and mitotic figures were not remarkable. Metachromasia was seen in cytoplasmic granules stained with toluidine blue. In the immunohistochemical staining, the neoplastic cells were positive for vimentin and c-kit. No immunoreactivity was seen for cytokeratin and HMB - 45. Based on these findings, multiple cutaneous mast cell tumor was diagnosed. Immunohistochemical features of mast cell tumor have not been reported previously in cattle.(AU)