Resumo
Solutions to induce or suppress the initiation of sperm motility in fish ha ve been used to improve reproductive success during artificial fertilization and preservation techniques . The aim of the present study was to evaluate the effects of three solutions (NaCl, glucose , and BTS) - each prepared with 10 different osmolalities - on the initiation and suppression of fresh sperm motility in Prochilodus lineat us and Brycon orbignyanus . Sperm was diluted in each of the 30 solution s and immediately observed under a light microscope to determine which solution s trigger ed or suppress ed the initiation of sperm motility. When present, motility rate ( % motile sperm ) w as determined at 0, 30 , and 120 s post - activation and the motility quality score ranging from 0 ( no movement ) to 5 ( rapidly swimming sperm) was determined at 0 and 30 s post - activation . Osmolality , but not solution composition , significantly affected both motility rate and quality score . Solutions at osmolali ties up to 270 mOsm/kg in P. lineatus and up to 180 mOsm/kg in B. orbignyanus induced motility in at least 60% of sperm , with a minimum quality score of 3.0 , and were therefore classified as activating agents. The greatest motility at 0 , 30 , and 120 s post - activation was observed with solutions ranging from 135 to 225 mOsm/kg for P. lineatus and at 135 mOsm/kg for B. orbignyanus . On the other hand, solutions ranging from 360 to 450 mOsm/kg in P. lineatus and 270 to 450 mOsm/kg in B. orbignyanus suppressed motility in at least 95% of sperm and were classified as immobilizing media . The osmolality of the surrounding medium is the key factor in the initiation or suppression of sperm motility in P. lineatus a nd B. orbignyanu.
Assuntos
Animais , Motilidade dos Espermatozoides/genética , Sêmen/citologia , Peixes/classificaçãoResumo
Solutions to induce or suppress the initiation of sperm motility in fish ha ve been used to improve reproductive success during artificial fertilization and preservation techniques . The aim of the present study was to evaluate the effects of three solutions (NaCl, glucose , and BTS) - each prepared with 10 different osmolalities - on the initiation and suppression of fresh sperm motility in Prochilodus lineat us and Brycon orbignyanus . Sperm was diluted in each of the 30 solution s and immediately observed under a light microscope to determine which solution s trigger ed or suppress ed the initiation of sperm motility. When present, motility rate ( % motile sperm ) w as determined at 0, 30 , and 120 s post - activation and the motility quality score ranging from 0 ( no movement ) to 5 ( rapidly swimming sperm) was determined at 0 and 30 s post - activation . Osmolality , but not solution composition , significantly affected both motility rate and quality score . Solutions at osmolali ties up to 270 mOsm/kg in P. lineatus and up to 180 mOsm/kg in B. orbignyanus induced motility in at least 60% of sperm , with a minimum quality score of 3.0 , and were therefore classified as activating agents. The greatest motility at 0 , 30 , and 120 s post - activation was observed with solutions ranging from 135 to 225 mOsm/kg for P. lineatus and at 135 mOsm/kg for B. orbignyanus . On the other hand, solutions ranging from 360 to 450 mOsm/kg in P. lineatus and 270 to 450 mOsm/kg in B. orbignyanus suppressed motility in at least 95% of sperm and were classified as immobilizing media . The osmolality of the surrounding medium is the key factor in the initiation or suppression of sperm motility in P. lineatus a nd B. orbignyanu.(AU)
Assuntos
Animais , Sêmen/citologia , Motilidade dos Espermatozoides/genética , Peixes/classificaçãoResumo
Studies regarding the effects of extender composition, osmolality, cryoprotectant (CPA) and equilibration time on the induction/suppression of sperm motility are necessary to establish standard activating agents and immobilizing media for improving both artificial fertilization and pr eservation techniques. Thus, the aim of this study was to evaluate the effects of these factors on fresh sperm motility in piracanjuba ( Brycon orbignyanus ) and streaked prochilod ( Prochilodus lineatus ). Twenty four media, as a combination of six extenders (BTS and glucose solutions at 270, 315 and 360 mOsm/kg) with the CPAs DMSO, methanol, methyl glycol (MG) and a control without CPA, were prepared. Immediately after dilution, samples were observed under a light microscope to confirm whether different extender-CPA combinations would suppress the initiation of sperm motility. Motility was then triggered in 92 mOsm/kg NaCl and evaluated immediately after dilution (non-equilibrated samples) and after a 30-min equilibration time at 4°C for motility rate and motility quality score (0 = no movement; 5 = rapidly swimming sperm). In both species, motility was initiated in all samples diluted in BTS-270-control, Glu-270-MG, Glu- 270-control and in all combinations containing DMSO. In B. orbignyanus , motility rate (77 to 92%) and motility quality score (3.3 to 4.7) of non-equilitrated samples was not significantly affected by any parameter. After 30 min, however, motility quality score decreased in most of the samples, mainly when diluted in BTS (3.3 to 4.2). In P. lineatus , motility rate was significantly higher in non-equilibrated samples (overall mean = 83%) compared to 30-min equilibrated samples (overall mean = 75%). Motility quality score of non- equilibrated samples was not affected by any parameter (3.3 to 4.2), but samples equilibrated in DMSO yielded the lowest score (3.0). Sperm motility (rate and score) was affected differently in B. orbignyanus compared to P. lineatus , and this finding should be considered when developing a methodology for sperm cryopreservation.
Assuntos
Animais , Motilidade dos Espermatozoides/genética , Reprodução/genética , Concentração Osmolar , Espermatozoides/citologiaResumo
Studies regarding the effects of extender composition, osmolality, cryoprotectant (CPA) and equilibration time on the induction/suppression of sperm motility are necessary to establish standard activating agents and immobilizing media for improving both artificial fertilization and pr eservation techniques. Thus, the aim of this study was to evaluate the effects of these factors on fresh sperm motility in piracanjuba ( Brycon orbignyanus ) and streaked prochilod ( Prochilodus lineatus ). Twenty four media, as a combination of six extenders (BTS and glucose solutions at 270, 315 and 360 mOsm/kg) with the CPAs DMSO, methanol, methyl glycol (MG) and a control without CPA, were prepared. Immediately after dilution, samples were observed under a light microscope to confirm whether different extender-CPA combinations would suppress the initiation of sperm motility. Motility was then triggered in 92 mOsm/kg NaCl and evaluated immediately after dilution (non-equilibrated samples) and after a 30-min equilibration time at 4°C for motility rate and motility quality score (0 = no movement; 5 = rapidly swimming sperm). In both species, motility was initiated in all samples diluted in BTS-270-control, Glu-270-MG, Glu- 270-control and in all combinations containing DMSO. In B. orbignyanus , motility rate (77 to 92%) and motility quality score (3.3 to 4.7) of non-equilitrated samples was not significantly affected by any parameter. After 30 min, however, motility quality score decreased in most of the samples, mainly when diluted in BTS (3.3 to 4.2). In P. lineatus , motility rate was significantly higher in non-equilibrated samples (overall mean = 83%) compared to 30-min equilibrated samples (overall mean = 75%). Motility quality score of non- equilibrated samples was not affected by any parameter (3.3 to 4.2), but samples equilibrated in DMSO yielded the lowest score (3.0). Sperm motility (rate and score) was affected differently in B. orbignyanus compared to P. lineatus , and this finding should be considered when developing a methodology for sperm cryopreservation.(AU)