An evaluation of farmers' perception, awareness, and adaptation towards climate change: a study from Punjab province Pakistan / Uma avaliação da percepção, consciência e adaptação dos agricultores em relação às mudanças climáticas: um estudo da província de Punjab, Paquistão

Globally, all environmental sectors are threatened by climate change, most especially to the food and agriculture sectors. Pakistan is a developing country that is most vulnerable to extreme and harsh climatic conditions, especially in Southeast Asia. Climate change has often adverse effects on Pakistan because it is often affected by severe weather conditions. Although, some studies have reported on farmers' perceptiveness regarding climate change adaptation and mitigation, there is still insufficient awareness among Pakistanis' farmers. Key knowledge is very important for farmers to overcome the harsh climatic conditions for harvesting and saving crops. To bridge this gap, this research discovered the awareness level of climate change, weather conditions, and related factors among Pakistani farmers. Through a stratified random sampling technique, 500 structured questionnaires were distributed among the farmers in four districts of Punjab Pakistan for study analysis. The probit model was used to analyze the farmer's perception regarding climate change, socio-demographic and economic variables. The findings of this research showed that 75% of farmers are aware of climate change. Perception of climate change between farmers was directly associated with agriculture experience, farmers' age, land ownership status, level of education, information sources, and access to extension. Also, our research showed that farmers' assessment of adaptive behavior showed that farmers are actively implementing crop diversification, irrigation, and other adaptation strategies. Results of this study will help government agencies and policymakers in Pakistan and other regions to develop sustainable adaptation measures in the framework of climate change.

Globalmente, todos os setores ambientais estão ameaçados pelas mudanças climáticas, principalmente para os setores de alimentos e agricultura. O Paquistão é um país em desenvolvimento mais vulnerável a condições climáticas extremas e adversas, especialmente no sudeste da Ásia. As mudanças climáticas costumam ter efeitos adversos no Paquistão, porque muitas vezes é afetado por condições climáticas severas. Embora alguns estudos relatem a percepção dos agricultores em relação à adaptação e mitigação das mudanças climáticas, ainda há consciência insuficiente entre os agricultores paquistaneses. O conhecimento chave é muito importante para os agricultores superarem as duras condições climáticas para a colheita e economia de safras. Para preencher essa lacuna, esta pesquisa pretende descobrir o nível de consciência das mudanças e condições climáticas e fatores relacionados entre os agricultores paquistaneses. Por meio de uma técnica de amostragem aleatória estratificada, 500 questionários estruturados foram distribuídos entre os agricultores em quatro distritos de Punjab Paquistão para análise do estudo. O modelo probit foi usado para analisar a percepção do agricultor em relação às mudanças climáticas, variáveis sócio-demográficas e econômicas. Os resultados desta pesquisa mostram que 75% dos agricultores estão cientes das mudanças climáticas. A percepção da mudança climática entre os agricultores foi diretamente associada à experiência agrícola, idade dos agricultores, situação de propriedade da terra, nível de educação, fontes de informação e acesso à extensão. Além disso, nossa pesquisa mostrou que a avaliação dos agricultores sobre o comportamento adaptativo mostra que eles estão implementando ativamente a diversificação de culturas, irrigação e outras estratégias de adaptação. Os resultados deste estudo ajudarão agências governamentais e formuladores de políticas no Paquistão e em outras regiões a desenvolver medidas de adaptação sustentáveis no contexto das mudanças climáticas.

Roles of the ERK1/2 and PI3K/PKB signaling pathways in regulating the expression of extracellular matrix genes in rat pulmonary artery smooth muscle cells

Purpose: To investigate the mechanisms by which PD98059 and LY294002 interfere with the abnormal deposition of extracellular matrix regulated by connective tissue growth factor (CTGF) of rat pulmonary artery smooth muscle cells (PASMCs). Methods: Rat PASMCs were cultured and separated into a control group. Real-time fluorescence quantitative PCR was performed to detect the expression of collagen III and fibronectin mRNA. Immunohistochemistry and western blot analyses were performed to detect the expression of collagen III protein. Results: The expression of collagen III and fibronectin mRNA was greater in PASMCs stimulated with CTGF for 48 h, than in the control group. After 72h of stimulation, the expression of collagen III protein in the PASMCs was greater than in the control. The equivalent gene and protein expression of the CPL group were much more significant. Conclusions: CTGF can stimulate the gene expression of collagen III and fibronectin in PASMCs, which may be one of the factors that promote pulmonary vascular remodeling (PVR) under the conditions of pulmonary arterial hypertension (PAH). PD98059 and LY294002 can inhibit the ERK1/2 and PI3K/PKB signaling pathways, respectively, thus interfering with the biological effects of CTGF. This may be a new way to reduce PAH-PVR.(AU)

Distribution of Vibrio alginolyticus-like species in Shenzhen coastal waters, China

We investigated the distribution of vibrios in Shenzhen coastal waters in order to obtain valuable information for the aquaculture industry and a health warning system. Quantities of vibrios from surface waters ranged from 0 to 4.40×10(4) CFUs mL-1 in April (spring), while from 0 to 2.57×10³ CFUs mL-1 in September (autumn); the abundance of V. alginolyticus-like species from surface water ranged from 0 to 6.72×10³ CFUs mL-1 in April (spring) and from 0 to 1.28×10³ CFUs mL-1 in September (autumn); higher counts were observed in spring. The V. alginolyticus-like species was dominant in Shenzhen coastal waters, with the highest abundance in the clean region (stations YMK001 and GDN064) in April, suggesting that Vibrio spp. were naturally occurring bacteria in marine environments. The correlation between the abundance of vibrios (including V. alginolyticus-like species) and environmental factors varied in different regions and different seasons. There were no vibrios detected when the salinity was less than 11.15‰ in the Zhujiang River estuary, which indicated that salinity played a key role in the distribution of vibrios and V. alginolyticus-like species.