Hepatic lipidosis due to obesity in a free-living snake (Boa constrictor amarali)

Background: Liver performs several important functions to the maintenance of physiological mechanisms. Some liver diseases may directly affect anatomical and physiological aspects of this organ, and may lead to a permanent liver injury. In snakes, the most common causes of liver disease are infections, however, approaches on non-infectious liver diseases are scarce. Therefore, the objective of this study was to describe macroscopically and microscopically liver alterations in a Boa constrictor amarali snake.Case: A adult male boa (Boa constrictor amarali) snake of 110 cm of length and weight of 3.270 kg from free-living conditions, and without previous history was rescued in an urban area and taken by the Environmental Police to the Laboratory for Teaching and Research in Wild Animals (LAPAS) of the Federal University of Uberlândia’s (UFU) Veterinary Hospital, in Uberlândia MG, Brazil. The animal died and a significant amount of adipose tissue was found throughout the extension of the coelomic cavity at necropsy, limiting the visualization of its internal organs. Fragments of altered organs were collected and packed in a universal collector containing a 10% buffered formalin solution. These samples were sent to the Animal Pathology Laboratory (LPA) of the UFU. Macroscopically, the stomach presented a reddish mucosa, and mucous contents. The liver was pale, with a yellowish color and a friable consistency. Microscopically, dilated hepatic sinusoids filled with red blood cells were observed; the hepatocytes were enlarged, and its cytoplasm were filled with vacuoles of varied sizes that did not stain (severe diffuse lipidosis). It was also found occurrence of multifocal areas with loss of tissue architecture, and hepatocytes in karyolysis, charactering necrosis; and a discrete amount of multifocal mononuclear inflammatory infiltrate (multifocal hepatitis).[...]

Hepatic lipidosis due to obesity in a free-living snake (Boa constrictor amarali)

Background: Liver performs several important functions to the maintenance of physiological mechanisms. Some liver diseases may directly affect anatomical and physiological aspects of this organ, and may lead to a permanent liver injury. In snakes, the most common causes of liver disease are infections, however, approaches on non-infectious liver diseases are scarce. Therefore, the objective of this study was to describe macroscopically and microscopically liver alterations in a Boa constrictor amarali snake.Case: A adult male boa (Boa constrictor amarali) snake of 110 cm of length and weight of 3.270 kg from free-living conditions, and without previous history was rescued in an urban area and taken by the Environmental Police to the Laboratory for Teaching and Research in Wild Animals (LAPAS) of the Federal University of Uberlândias (UFU) Veterinary Hospital, in Uberlândia MG, Brazil. The animal died and a significant amount of adipose tissue was found throughout the extension of the coelomic cavity at necropsy, limiting the visualization of its internal organs. Fragments of altered organs were collected and packed in a universal collector containing a 10% buffered formalin solution. These samples were sent to the Animal Pathology Laboratory (LPA) of the UFU. Macroscopically, the stomach presented a reddish mucosa, and mucous contents. The liver was pale, with a yellowish color and a friable consistency. Microscopically, dilated hepatic sinusoids filled with red blood cells were observed; the hepatocytes were enlarged, and its cytoplasm were filled with vacuoles of varied sizes that did not stain (severe diffuse lipidosis). It was also found occurrence of multifocal areas with loss of tissue architecture, and hepatocytes in karyolysis, charactering necrosis; and a discrete amount of multifocal mononuclear inflammatory infiltrate (multifocal hepatitis).[...](AU)

Odontogenic tumor, cholangioarcinoma and gastric fibroma in a dog – case report

Odontogenic tumors producing amyloid are rare neoplasms. Cholangiocarcinomas and fibromas are also referred to as infrequent. The objective of this study was to report the case of a dog, 17 years old, a poodle, who was treated at the Veterinary Hospital of the Federal University of Uberlândia. At necropsy, the animal presented only nodule located in the oral cavity, in the same way, only nodule in the hepatic parenchyma and in addition, multiple masses in the stomach. Microscopically, the histological features were compatible with odontogenic tumor producing amyloid, cholangiocarcinoma and fibroma, respectively. Reports of simultaneous occurrence of distinct tumors are scarce in the literature.

Os tumores odontogênicos produtores de amiloide são neoplasias raras. Os colangiocarcinomas e fibromas também são referidos como infrequentes. Objetivou-se relatar o caso de um cão, 17 anos, poodle, que foi atendido no Hospital Veterinário da Universidade Federal de Uberlândia. À necropsia, o animal apresentou único nódulo localizado na cavidade oral, da mesma forma, único nódulo no parênquima hepático e ademais, múltiplas massas no estômago. Microscopicamente, as características histológicas foram compatíveis com tumor odontogênico produtor de amiloide, colangiocarcinoma e fibroma, respectivamente. Relatos de ocorrência simultânea de tumores distintos são escassos na literatura.

Odontogenic tumor, cholangioarcinoma and gastric fibroma in a dog case report

Odontogenic tumors producing amyloid are rare neoplasms. Cholangiocarcinomas and fibromas are also referred to as infrequent. The objective of this study was to report the case of a dog, 17 years old, a poodle, who was treated at the Veterinary Hospital of the Federal University of Uberlândia. At necropsy, the animal presented only nodule located in the oral cavity, in the same way, only nodule in the hepatic parenchyma and in addition, multiple masses in the stomach. Microscopically, the histological features were compatible with odontogenic tumor producing amyloid, cholangiocarcinoma and fibroma, respectively. Reports of simultaneous occurrence of distinct tumors are scarce in the literature.(AU)

Os tumores odontogênicos produtores de amiloide são neoplasias raras. Os colangiocarcinomas e fibromas também são referidos como infrequentes. Objetivou-se relatar o caso de um cão, 17 anos, poodle, que foi atendido no Hospital Veterinário da Universidade Federal de Uberlândia. À necropsia, o animal apresentou único nódulo localizado na cavidade oral, da mesma forma, único nódulo no parênquima hepático e ademais, múltiplas massas no estômago. Microscopicamente, as características histológicas foram compatíveis com tumor odontogênico produtor de amiloide, colangiocarcinoma e fibroma, respectivamente. Relatos de ocorrência simultânea de tumores distintos são escassos na literatura.(AU)

Prognostic factors in canine mammary carcinomas and HER2 expression relationship / Fatores de prognóstico em carcinomas mamários caninos e sua relação com expressão de HER2

Background: The human epidermal growth factor type 2 (HER2) receptor is a membrane glycoprotein tyrosine kinase. In woman, HER2 expression is diagnosed in 30% of breast carcinomas and it is associated with a worse prognosis, higher rate of recurrence and mortality. In the bitch, the HER2 overexpression in canine mammary tumors is still controversial and the prognostic value remains uncertain. Thus, we aimed to verify the HER2 expression in canine mammary carcinomas and relate it to the type and histological grade, lymph node metastasis and clinical staging. Materials, Methods & Results: Ninety bitches diagnosed with mammary carcinoma were included in this study. The inclusion criteria were bitches with complete clinical examination, thoracic radiographic examination and submitted unilateral or bilateral mastectomy. Ninety-nine samples of mammary carcinoma were used and the fragments of tumor and regional lymph nodes were fixed in 10% neutral formalin for histopathological and immunohistochemistry analysis. The lesions were evaluated by two pathologists and classified according to the type and histological grade. HER2 expression was performed by semi-quantitative analysis of the slides according to the HerceptTestTM (Dako) recommended score. Simple carcinomas were the most frequent (51.51%) followed by complex carcinomas (46.47%) and in situ carcinoma (2.02%). [...](AU)

Hemangiomas and hemangiosarcomas in dogs: retrospective study of 192 cases (2002-2014) / Hemangiomas e hemangiossarcomas em cães: estudo retrospectivo de 192 casos (2002-2014)

The hemangioma and hemangiosarcoma are mesenchymal neoplasms from the vascular endothelium, and the dog is the domestic animal more commonly affected. At the veterinary hospital of the Federal University of Uberlândia, we reviewed the files from the Animal Pathology Laboratory from 2002 to 2014. The epidemiological data and the frequency of dogs diagnosed with hemangioma and hemangiosarcoma were verified, correlated, and statistically tested. 64 cases of hemangioma and 128 cases of hemangiosarcoma were identified. For hemangiomas, there was no difference in frequency regarding sex and breed. The cutaneous hemangiomas were more frequent than visceral hemangiomas. Female dogs and adult animals were often affected by hemangiosarcoma, as well as the Pitbull breed. The hemangiosarcomas had been predominantly cutaneous and medium size tumour was the most frequent for this neoplasm.

O hemangioma e o hemangiossarcoma são neoplasias mesenquimais originárias do endotélio vascular e o cão é o animal doméstico mais frequentemente acometido. Os registros do Laboratório de Patologia Animal do Hospital Veterinário da Universidade Federal de Uberlândia de 2002 a 2014 foram revisados. Os dados epidemiológicos e a frequência de cães com diagnóstico de hemangioma e hemangiossarcoma foram verificados e estatisticamente comparados. Foram identificados 64 casos de hemangioma e 128 casos de hemangiossarcoma. Para os hemangiomas, não houve diferença na frequência de acordo com sexo e raça. Quanto à localização, os hemangiomas não viscerais foram os mais frequentes em relação aos viscerais. As cadelas e animais adultos e idosos foram frequentemente acometidos por hemangiossarcoma, assim como a raça Pitbull. Os hemangiossarcomas apresentaram predominantemente tamanho médio e localização cutânea, ou seja, não visceral.

Prognostic factors in canine mammary carcinomas and HER2 expression relationship / Fatores de prognóstico em carcinomas mamários caninos e sua relação com expressão de HER2

Background: The human epidermal growth factor type 2 (HER2) receptor is a membrane glycoprotein tyrosine kinase. In woman, HER2 expression is diagnosed in 30% of breast carcinomas and it is associated with a worse prognosis, higher rate of recurrence and mortality. In the bitch, the HER2 overexpression in canine mammary tumors is still controversial and the prognostic value remains uncertain. Thus, we aimed to verify the HER2 expression in canine mammary carcinomas and relate it to the type and histological grade, lymph node metastasis and clinical staging. Materials, Methods & Results: Ninety bitches diagnosed with mammary carcinoma were included in this study. The inclusion criteria were bitches with complete clinical examination, thoracic radiographic examination and submitted unilateral or bilateral mastectomy. Ninety-nine samples of mammary carcinoma were used and the fragments of tumor and regional lymph nodes were fixed in 10% neutral formalin for histopathological and immunohistochemistry analysis. The lesions were evaluated by two pathologists and classified according to the type and histological grade. HER2 expression was performed by semi-quantitative analysis of the slides according to the HerceptTestTM (Dako) recommended score. Simple carcinomas were the most frequent (51.51%) followed by complex carcinomas (46.47%) and in situ carcinoma (2.02%). [...]

Prognostic Factors in Canine Mammary Carcinomas and HER2 Expression Relationship

Background: The human epidermal growth factor type 2 (HER2) receptor is a membrane glycoprotein tyrosine kinase. In woman, HER2 expression is diagnosed in 30% of breast carcinomas and it is associated with a worse prognosis, higher rate of recurrence and mortality. In the bitch, the HER2 overexpression in canine mammary tumors is still controversial and the prognostic value remains uncertain. Thus, we aimed to verify the HER2 expression in canine mammary carcinomas and relate it to the type and histological grade, lymph node metastasis and clinical staging.Materials, Methods & Results: Ninety bitches diagnosed with mammary carcinoma were included in this study. The inclusion criteria were bitches with complete clinical examination, thoracic radiographic examination and submitted unilateral or bilateral mastectomy. Ninety-nine samples of mammary carcinoma were used and the fragments of tumor and regional lymph nodes were fixed in 10% neutral formalin for histopathological and immunohistochemistry analysis. The lesions were evaluated by two pathologists and classified according to the type and histological grade. HER2 expression was performed by semi-quantitative analysis of the slides according to the HerceptTestTM (Dako) recommended score. Simple carcinomas were the most frequent (51.51%) followed by complex carcinomas (46.47%) and in situ carcinoma (2.02%). The histologica

Linfomas em cães: aspectos epidemiológicos / Lymphoma in dogs: Epidemiological aspects

Com os avanços na Medicina Veterinária, houve um aumento na expectativa de vida dos animais de companhia, e em contrapartida,a incidência de doenças crônicas como o câncer tem aumentado nestes animais. O linfoma é uma neoplasia maligna de linfócitoscomumente encontrada nos caninos e sua incidência é progressiva, em torno de 24 a 33 casos por ano para cada 100.000 cães.Foram consultadas as fichas do Setor de Patologia Animal da Universidade Federal de Uberlândia (UFU), no período compreendidoentre janeiro de 2003 e dezembro de 2013, para avaliar a frequência de linfoma nos cães assistidos no Hospital Veterinário (UFU)neste período. Informações como o tipo de exame realizado, sexo, idade, raça e tipo de amostra foram coletadas. Também foiavaliada a frequência do linfoma entre os tumores de células redondas. Posteriormente, os dados foram comparados utilizandoseestatística descritiva e porcentual. De todos os 6403 registros, 70,3% dos diagnósticos de linfoma foram observados por meiode citologia e 29,6% por histopatologia. O linfoma foi o segundo tumor de células redondas mais frequente em 23,1%. Não houvepredileção sexual e os cães adultos e idosos representaram as faixas etárias mais afetadas. A raça Pit Bull foi a mais frequenteem 28,2% dos casos. Em 53,3% dos diagnósticos citológicos conclusivos sem necessidade de biópsia, os cães apresentaramlinfadenomegalia na avaliação clínica. A citologia pode ser uma boa ferramenta no diagnóstico de linfomas, auxiliado pela avaliaçãofísica dos linfonodos superficiais.(AU)

With advances in veterinary medicine, there was an increase in life expectancy of pets, and on the other hand, the incidence ofchronic diseases such as cancer has increased in these animals. Lymphoma is a malignant neoplasm of lymphocytes commonlyfound in canine and its incidence is progressive, around 24-33 cases per year for each 100.000 dogs. The reports of the Sectorof Animal Pathology, Federal University of Uberlândia (UFU), were consulted in the period between January 2003 and December2013 to assess the frequency of lymphoma in dogs assisted at the Veterinary Hospital (UFU) in this period. Information such asthe type of examination, gender, age, and type of sample was collected. We also evaluated the frequency of lymphoma betweenthe round cell tumors. Later, the data were compared using descriptive statistics and percentage. Of all 6403 records, 70.3% oflymphoma diagnoses were observed by cytology and 29.6% by histopathology. Lymphoma was the second most frequent roundcell tumor in 23.1%. There was no sexual predilection and the adult and elderly dogs represented the most affected age groups.The Pit Bull breed was the most frequent in 28.2% of the cases. In 53.3% of the conclusive cytologic diagnoses without biopsy, thedogs presented lymphadenomegaly in the clinical evaluation. Cytology can be a good tool in the diagnosis of lymphomas, aidedby the physical evaluation of superficial lymph nodes.(AU)

Hemangiomas and hemangiosarcomas in dogs: retrospective study of 192 cases (2002-2014) / Hemangiomas e hemangiossarcomas em cães: estudo retrospectivo de 192 casos (2002-2014)

The hemangioma and hemangiosarcoma are mesenchymal neoplasms from the vascular endothelium, and the dog is the domestic animal more commonly affected. At the veterinary hospital of the Federal University of Uberlândia, we reviewed the files from the Animal Pathology Laboratory from 2002 to 2014. The epidemiological data and the frequency of dogs diagnosed with hemangioma and hemangiosarcoma were verified, correlated, and statistically tested. 64 cases of hemangioma and 128 cases of hemangiosarcoma were identified. For hemangiomas, there was no difference in frequency regarding sex and breed. The cutaneous hemangiomas were more frequent than visceral hemangiomas. Female dogs and adult animals were often affected by hemangiosarcoma, as well as the Pitbull breed. The hemangiosarcomas had been predominantly cutaneous and medium size tumour was the most frequent for this neoplasm.(AU)

O hemangioma e o hemangiossarcoma são neoplasias mesenquimais originárias do endotélio vascular e o cão é o animal doméstico mais frequentemente acometido. Os registros do Laboratório de Patologia Animal do Hospital Veterinário da Universidade Federal de Uberlândia de 2002 a 2014 foram revisados. Os dados epidemiológicos e a frequência de cães com diagnóstico de hemangioma e hemangiossarcoma foram verificados e estatisticamente comparados. Foram identificados 64 casos de hemangioma e 128 casos de hemangiossarcoma. Para os hemangiomas, não houve diferença na frequência de acordo com sexo e raça. Quanto à localização, os hemangiomas não viscerais foram os mais frequentes em relação aos viscerais. As cadelas e animais adultos e idosos foram frequentemente acometidos por hemangiossarcoma, assim como a raça Pitbull. Os hemangiossarcomas apresentaram predominantemente tamanho médio e localização cutânea, ou seja, não visceral.(AU)

HEMANGIOMAS E HEMANGIOSSARCOMAS EM CÃES: ESTUDO RETROSPECTIVO DE 192 CASOS (2002-2014)

Abstract The hemangioma and hemangiosarcoma are mesenchymal neoplasms from the vascular endothelium, and the dog is the domestic animal more commonly affected. At the veterinary hospital of the Federal University of Uberlândia, we reviewed the files from the Animal Pathology Laboratory from 2002 to 2014. The epidemiological data and the frequency of dogs diagnosed with hemangioma and hemangiosarcoma were verified, correlated, and statistically tested. 64 cases of hemangioma and 128 cases of hemangiosarcoma were identified. For hemangiomas, there was no difference in frequency regarding sex and breed. The cutaneous hemangiomas were more frequent than visceral hemangiomas. Female dogs and adult animals were often affected by hemangiosarcoma, as well as the Pitbull breed. The hemangiosarcomas had been predominantly cutaneous and medium size tumour was the most frequent for this neoplasm.

Resumo O hemangioma e o hemangiossarcoma são neoplasias mesenquimais originárias do endotélio vascular e o cão é o animal doméstico mais frequentemente acometido. Os registros do Laboratório de Patologia Animal do Hospital Veterinário da Universidade Federal de Uberlândia de 2002 a 2014 foram revisados. Os dados epidemiológicos e a frequência de cães com diagnóstico de hemangioma e hemangiossarcoma foram verificados e estatisticamente comparados. Foram identificados 64 casos de hemangioma e 128 casos de hemangiossarcoma. Para os hemangiomas, não houve diferença na frequência de acordo com sexo e raça. Quanto à localização, os hemangiomas não viscerais foram os mais frequentes em relação aos viscerais. As cadelas e animais adultos e idosos foram frequentemente acometidos por hemangiossarcoma, assim como a raça Pitbull. Os hemangiossarcomas apresentaram predominantemente tamanho médio e localização cutânea, ou seja, não visceral.

Evaluación imunohistoquímica de plasmocitoma cutáneo en un perro - reporte de un caso / Assessment immunohistochemistry of the cutaneous plasmacytoma in a dog - case report / Avaliação imunohistoquímica de plasmocitoma cutâneo em um cão: relato de caso

Los plasmacitomas son neoplasias poco comunes em los perros, procedentes de plasmocitos. Ocurre en cualquier tejido blando, pero principalmente em la piel, cavidad oral y el tracto digestivo. Se describe un caso de plasmocitoma anaplásico cutâneo em un perro, mestizo, de 16 años de edad. El animal mostro múltiples nódulos de color rosado, irregulares y de consistência blanda, extendidos em la piel. Em elexamen histopatológico se observo área circunscrita consistente en células redondas que muestran intensa anisocariosis, anisocitosis y número moderado de células gigantes, multinucleadas. La evaluación imunohistoquímica reveló expresión de CD79a y MUM1, lo que permite el diagnóstico de plasmocitoma anaplásico.(AU)

Plasmacytomas are uncommon neoplasms in dogs originating in the plasma cells.Occur in any soft tissue, but mainly in the skin, oral cavity and digestive tract. We reported a case of cutaneous anaplastic plasmocytoma in a dog, mixed breed, 16 years old. The animal showed multiple nodules of pinkish color, rough and smooth consistency, spread on the skin. In the histopathological examination revealed circumscribed area consisting of round cells showing intense anisocytosis, anisokaryosis and moderate number of giant cells, multinucleated. The immunohistochemical demonstrated expression for MUM1 and CD79a, allowing diagnosis of anaplastic plasmacytoma.(AU)

Plasmocitomas são neoplasias incomuns no cão, originárias dos plasmócitos. Ocorrem em qualquer tecido mole, mas principalmente na pele, cavidade oral e trato digestivo. Relata-se um caso de plasmocitoma anaplásico cutâneo em um cão, mestiço, de 16 anos. O animal apresentava vários nódulos de coloração rósea, irregulares e de consistência macia, distribuídos na pele. Na análise histopatológica observou-se área circunscrita constituída de células redondas exibindo intensa anisocitose, anisocariose e moderada quantidade de células gigantes, multinucleadas. A avaliação imunohistoquímica revelou expressão para MUM1 e CD79a, permitindo o diagnóstico de plasmocitoma anaplásico.(AU)

Evaluación imunohistoquímica de plasmocitoma cutáneo en un perro - reporte de un caso / Assessment immunohistochemistry of the cutaneous plasmacytoma in a dog - case report / Avaliação imunohistoquímica de plasmocitoma cutâneo em um cão: relato de caso

Los plasmacitomas son neoplasias poco comunes em los perros, procedentes de plasmocitos. Ocurre en cualquier tejido blando, pero principalmente em la piel, cavidad oral y el tracto digestivo. Se describe un caso de plasmocitoma anaplásico cutâneo em un perro, mestizo, de 16 años de edad. El animal mostro múltiples nódulos de color rosado, irregulares y de consistência blanda, extendidos em la piel. Em elexamen histopatológico se observo área circunscrita consistente en células redondas que muestran intensa anisocariosis, anisocitosis y número moderado de células gigantes, multinucleadas. La evaluación imunohistoquímica reveló expresión de CD79a y MUM1, lo que permite el diagnóstico de plasmocitoma anaplásico.

Plasmacytomas are uncommon neoplasms in dogs originating in the plasma cells.Occur in any soft tissue, but mainly in the skin, oral cavity and digestive tract. We reported a case of cutaneous anaplastic plasmocytoma in a dog, mixed breed, 16 years old. The animal showed multiple nodules of pinkish color, rough and smooth consistency, spread on the skin. In the histopathological examination revealed circumscribed area consisting of round cells showing intense anisocytosis, anisokaryosis and moderate number of giant cells, multinucleated. The immunohistochemical demonstrated expression for MUM1 and CD79a, allowing diagnosis of anaplastic plasmacytoma.

Plasmocitomas são neoplasias incomuns no cão, originárias dos plasmócitos. Ocorrem em qualquer tecido mole, mas principalmente na pele, cavidade oral e trato digestivo. Relata-se um caso de plasmocitoma anaplásico cutâneo em um cão, mestiço, de 16 anos. O animal apresentava vários nódulos de coloração rósea, irregulares e de consistência macia, distribuídos na pele. Na análise histopatológica observou-se área circunscrita constituída de células redondas exibindo intensa anisocitose, anisocariose e moderada quantidade de células gigantes, multinucleadas. A avaliação imunohistoquímica revelou expressão para MUM1 e CD79a, permitindo o diagnóstico de plasmocitoma anaplásico.

Comparison of complementary diagnostic methods of bovine tuberculosis on skintest reactive cattle / Exames complementares no diagnóstico da tuberculose em bovinos reagentes à tuberculinização comparada

The aim of this study was to compare different complementary diagnostic methods (macroscopic, histological, serological and molecular) of tuberculosis in naturally infected cattle. The study was conducted from samples taken from 40 reagents cattle in cervical comparative intradermal tuberculin test  (ITT). The macroscopic post mortem inspection of carcasses was followed by the collection of nasal swabs, blood and tissue samples (liver, lung and mediastinal lymph node) for polymerase chain reaction (PCR) test, EnzymeLinked Immunosorbent Assay (ELISA) and histopathologic test with hematoxylineosin (HE) and ZiehlNeelsen (ZN), respectively. Of the 40 cattle slaughtered, 22 (55%) carcasses had macroscopic lesions suggestive of tuberculosis. The histopathology HE identified 13 (32.5%) carcasses with histological changes: 6 (15%) in the mediastinal lymph nodes, 5 (12.5%) in the liver and 3 (7.5%) in the lung. At  ZN, the presence of acidfast bacilli was not detected in any of the samples tested. The ELISA/IDEXX® identified one (2.5%) reagent animal, and the PCR test detected DNA of Mycobacterium bovis in one (2.5%) cow. It is concluded that among the diagnostic methods reviews none was able to detect all animals that were positive in tuberculin test, but the association of different methods can ensure diagnostic accuracy.

Objetivou-se com este estudo comparar diferentes métodos complementares de diagnóstico (macroscópico, histopatológico, sorológico e molecular) da tuberculose, em bovinos naturalmente infectados. O trabalho deu-se por meio de amostras colhidas em abate sanitário de 40 bovinos reagentes no teste cervical comparativo (TCC) para tuberculose. A inspeção macroscópica post mor tem das carcaças foi acompanhada de colheita de amostras de muco nasal, sangue e tecido (fígado, pulmão e linfonodo mediastínico) para realização do exame de reação em cadeia pela polimerase (PCR), de EnzymeLinked Immunosorbent Assay (ELISA) e histopatológico com colorações de hematoxilinaeosina (HE) e ZiehlNeelsen (ZN), respectivamente. Dos 40 bovinos inspecionados no abate, 22 (55%) animais apresentaram algum tipo de lesão macroscópica sugestiva de tuberculose. Nos achados histopatológicos visualizados em HE, 13 (32,5%) carcaças apontaram alterações histológicas, sendo 6 (15%) nos linfonodos mediastínicos, 5 (12,5%) no fígado e 3 (7,5%) no pulmão. Não foi observada a presença de bacilos álcool-ácido resistentes em nenhuma das amostras avaliadas. O ensaio sorológico de ELISA/IDEXX® identificou um (2,5%) animal reagente, e o teste de PCR detectou DNA de Mycobacterium bovis em uma (2,5%) amostra. Concluiu-se que entre os exames complementares de diagnóstico avaliados nenhum foi capaz de detectar todos os animais que estavam positivos na tuberculinização, porém a associação de diferentes métodos pode garantir a confiabilidade do diagnóstico.

Exames complementares no diagnóstico da tuberculose em bovinos reagentes à tuberculinização comparada / Comparison of complementary diagnostic methods of bovine tuberculosis on skin-test reactive cattle

Objetivou-se com este estudo comparar diferentes métodos complementares de diagnóstico (macroscópico, histopatológico, sorológico e molecular) da tuberculose, em bovinos naturalmente infectados. O trabalho deu-se por meio de amostras colhidas em abate sanitário de 40 bovinos reagentes no teste cervical comparativo (TCC) para tuberculose. A inspeção macroscópica post mortem das carcaças foi acompanhada de colheita de amostras de muco nasal, sangue e tecido (fígado, pulmão e linfonodo mediastínico) para realização do exame de reação em cadeia pela polimerase (PCR), de Enzyme-Linked Immunosorbent Assay (ELISA) e histopatológico com colorações de hematoxilina-eosina (HE) e Ziehl-Neelsen (ZN), respectivamente. Dos 40 bovinos inspecionados no abate, 22 (55%) animais apresentaram algum tipo de lesão macroscópica sugestiva de tuberculose. Nos achados histopatológicos visualizados em HE, 13 (32,5%) carcaças apontaram alterações histológicas, sendo 6 (15%) nos linfonodos mediastínicos, 5 (12,5%) no fígado e 3 (7,5%) no pulmão. Não foi observada a presença de bacilos álcool-ácido resistentes em nenhuma das amostras avaliadas. O ensaio sorológico de ELISA/IDEXX(r) identificou um (2,5%) animal reagente, e o teste de PCR detectou DNA de Mycobacterium bovis em uma (2,5%) amostra. Concluiu-se que entre os exames complementares de diagnóstico avaliados nenhum foi capaz de detectar todos os animais que estavam positivos na tuberculinização, porém a associação de diferentes métodos pode garantir a confiabilidade do diagnóstico.(AU)

The aim of this study was to compare different complementary diagnostic methods (macroscopic, histological, serological and molecular) of tuberculosis in naturally infected cattle. The study was conducted from samples taken from 40 reagents cattle in cervical comparative intradermal tuberculin test (ITT). The macroscopic post mortem inspection of carcasses was followed by the collection of nasal swabs, blood and tissue samples (liver, lung and mediastinal lymph node) for polymerase chain reaction (PCR) test, Enzyme-Linked Immunosorbent Assay (ELISA) and histopathologic test with hematoxylin-eosin (HE) and Ziehl-Neelsen (ZN), respectively. Of the 40 cattle slaughtered, 22 (55%) carcasses had macroscopic lesions suggestive of tuberculosis. The histopathology HE identified 13 (32.5%) carcasses with histological changes: 6 (15%) in the mediastinal lymph nodes, 5 (12.5%) in the liver and 3 (7.5%) in the lung. At ZN, the presence of acid-fast bacilli was not detected in any of the samples tested. The ELISA/IDEXX(r) identified one (2.5%) reagent animal, and the PCR test detected DNA of Mycobacterium bovis in one (2.5%) cow. It is concluded that among the diagnostic methods reviews none was able to detect all animals that were positive in tuberculin test, but the association of different methods can ensure diagnostic accuracy.(AU)

Comparison of complementary diagnostic methods of bovine tuberculosis on skintest reactive cattle / Exames complementares no diagnóstico da tuberculose em bovinos reagentes à tuberculinização comparada

The aim of this study was to compare different complementary diagnostic methods (macroscopic, histological, serological and molecular) of tuberculosis in naturally infected cattle. The study was conducted from samples taken from 40 reagents cattle in cervical comparative intradermal tuberculin test  (ITT). The macroscopic post mortem inspection of carcasses was followed by the collection of nasal swabs, blood and tissue samples (liver, lung and mediastinal lymph node) for polymerase chain reaction (PCR) test, EnzymeLinked Immunosorbent Assay (ELISA) and histopathologic test with hematoxylineosin (HE) and ZiehlNeelsen (ZN), respectively. Of the 40 cattle slaughtered, 22 (55%) carcasses had macroscopic lesions suggestive of tuberculosis. The histopathology HE identified 13 (32.5%) carcasses with histological changes: 6 (15%) in the mediastinal lymph nodes, 5 (12.5%) in the liver and 3 (7.5%) in the lung. At  ZN, the presence of acidfast bacilli was not detected in any of the samples tested. The ELISA/IDEXX® identified one (2.5%) reagent animal, and the PCR test detected DNA of Mycobacterium bovis in one (2.5%) cow. It is concluded that among the diagnostic methods reviews none was able to detect all animals that were positive in tuberculin test, but the association of different methods can ensure diagnostic accuracy.(AU)

Objetivou-se com este estudo comparar diferentes métodos complementares de diagnóstico (macroscópico, histopatológico, sorológico e molecular) da tuberculose, em bovinos naturalmente infectados. O trabalho deu-se por meio de amostras colhidas em abate sanitário de 40 bovinos reagentes no teste cervical comparativo (TCC) para tuberculose. A inspeção macroscópica post mor tem das carcaças foi acompanhada de colheita de amostras de muco nasal, sangue e tecido (fígado, pulmão e linfonodo mediastínico) para realização do exame de reação em cadeia pela polimerase (PCR), de EnzymeLinked Immunosorbent Assay (ELISA) e histopatológico com colorações de hematoxilinaeosina (HE) e ZiehlNeelsen (ZN), respectivamente. Dos 40 bovinos inspecionados no abate, 22 (55%) animais apresentaram algum tipo de lesão macroscópica sugestiva de tuberculose. Nos achados histopatológicos visualizados em HE, 13 (32,5%) carcaças apontaram alterações histológicas, sendo 6 (15%) nos linfonodos mediastínicos, 5 (12,5%) no fígado e 3 (7,5%) no pulmão. Não foi observada a presença de bacilos álcool-ácido resistentes em nenhuma das amostras avaliadas. O ensaio sorológico de ELISA/IDEXX® identificou um (2,5%) animal reagente, e o teste de PCR detectou DNA de Mycobacterium bovis em uma (2,5%) amostra. Concluiu-se que entre os exames complementares de diagnóstico avaliados nenhum foi capaz de detectar todos os animais que estavam positivos na tuberculinização, porém a associação de diferentes métodos pode garantir a confiabilidade do diagnóstico.(AU)

Grau de malignidade do mastocitoma cutâneo canino quanto a localização segundo as classificações de Patnaik et al. (1984) e Kiupel et al. (2011) / Degree of malignancy of the canine cutaneous mast cell tumor as forlocation according to the classifications of Patnaik et al. (1984) and Kiupel et al. (2011)

Objetivou-se com o presente trabalho coletar dados epidemiológicos relacionados com o mastocitoma canino, além de verificar se há relação entre sua malignidade e localização e, por fim, comparar os métodos de classificação histopatológica segundo Patnaik et al. (1984) e segundo Kiupel et al. (2011). Informações foram coletadas da ficha clínica de 55 cães e 60 fragmentos de pele com mastocitomas foram avaliados histologicamente. Verificou-se que a ocorrência de mastocitoma cutâneo não é influenciada pelo sexo e raça, porém cães sem raça definida e boxers são mais acometidos. Não há uma faixa etária susceptível bem definida, sendo o mastocitoma mais frequente em cães de 8 a 9 anos de idade. A região mais acometida foi a inguinal (50%) e a cabeça a região que apresentou mastocitomas com maior malignidade. Utilizando a classificação de Patnaik et al. (1984) houve diferença significativa entre as classificações histopatológicas de mastocitoma avaliadas por diferentes patologistas, o que não ocorreu utilizando a classificação de Kiupel et al. (2011). Pode-se dizer então que a classificação de Kiupel et al. (2011) gera menor divergência nos diagnósticos, demonstrando-se um método simples e eficaz para a avaliação histopatológica de mastocitoma.(AU)

The aim of the present study is to collect epidemiological data related to the canine mastocytoma, to check if there is relationbetween malignancy and its location and, finally, to compare the methods of histopathological classification according to Patnaiket al. (1984) and Kiupel et al. (2011). Informations were collected from the clinical record of 55 dogs and 60 fragments of skinwith a diagnosis of mast cell tumor were evaluated histologically. It was found that the occurrence of cutaneous mast cell tumorsis not influenced by gender and race, but dogs without defined breed and boxers are most affected. There is not a well-definedsusceptible age, but the most mast cell tumors in dogs often 8 to 9 years old. The highest affected region was inguinal (50%) andhead proved a region with higher malignancy. Using the classification Patnaik et al. (1984) there was a significant difference betweenthe histopathological ratings mastocytoma evaluated by different pathologists, which did not occur using the classification Kiupel etal. (2011). It can be said then that the classification Kiupel et al. (2011) generates less divergence in the diagnosis, demonstratinga simple and effective method for histopathological evaluation of mastocytoma.(AU)

Immunohistochemistry in myocardium of dogs naturally infected with Leishmania chagasi / Imunohistoquímica em miocárdio de cães naturamente infectados por Leishmania chagasi

Background: Canine visceral leishmaniasis is a chronic and endemic disease, widespread zoonotic infection with a greatimpact on public health. The domestic dog is the main reservoir and also a source of infection for humans in the urbanenvironment. Canine visceral leishmaniasis is a multisystem disease that affects several organs, mainly the heart. The aimof this study was to verify the presence of amastigotes of Leishmania chagasi in myocardium of dogs naturally infectedby immunohistochemistry. In addition, to correlate the number of parasitized cells with the intensity of the inflammation,the macroscopic and microscopic lesions.Materials, Methods & Results: It were used 52 dogs positive for leishmaniasis diagnosed by clinical examination, IndirectImmunofluorescence (RIFI), ELISA and cytological analysis of popliteal lymph nodes. The positive animals were euthanized and submitted a necropsy. Following necropsy, the macroscopic analysis was done, and samples from the region ofthe cardiac apex were collected at the transition between the two ventricles. Fragments of heart tissues were fixed in 10%neutral formalin for histopathological and immunohistochemistry analysis. The inflammatory process of the myocardiumand lesions semi-quantitatively scored for myocardial injury on a scale of 0 to 3: (0) absent, (1) mild = small focal lesions,(2) moderate = larger, multifocal lesions and (3) severe = affecting most areas with coalescence. On immunostaining,the animal was classified according to the parasite load: - (no microorganism), mild (1-100 microorganisms), moderate(101-300), intense parasitism (> 300). At the macroscopically analysis, 33 animals (63.46%) had at least one lesion. Theconcentric hypertrophy left ventricular was the most...(AU)

Immunohistochemistry in myocardium of dogs naturally infected with Leishmania chagasi / Imunohistoquímica em miocárdio de cães naturamente infectados por Leishmania chagasi

Background: Canine visceral leishmaniasis is a chronic and endemic disease, widespread zoonotic infection with a greatimpact on public health. The domestic dog is the main reservoir and also a source of infection for humans in the urbanenvironment. Canine visceral leishmaniasis is a multisystem disease that affects several organs, mainly the heart. The aimof this study was to verify the presence of amastigotes of Leishmania chagasi in myocardium of dogs naturally infectedby immunohistochemistry. In addition, to correlate the number of parasitized cells with the intensity of the inflammation,the macroscopic and microscopic lesions.Materials, Methods & Results: It were used 52 dogs positive for leishmaniasis diagnosed by clinical examination, IndirectImmunofluorescence (RIFI), ELISA and cytological analysis of popliteal lymph nodes. The positive animals were euthanized and submitted a necropsy. Following necropsy, the macroscopic analysis was done, and samples from the region ofthe cardiac apex were collected at the transition between the two ventricles. Fragments of heart tissues were fixed in 10%neutral formalin for histopathological and immunohistochemistry analysis. The inflammatory process of the myocardiumand lesions semi-quantitatively scored for myocardial injury on a scale of 0 to 3: (0) absent, (1) mild = small focal lesions,(2) moderate = larger, multifocal lesions and (3) severe = affecting most areas with coalescence. On immunostaining,the animal was classified according to the parasite load: - (no microorganism), mild (1-100 microorganisms), moderate(101-300), intense parasitism (> 300). At the macroscopically analysis, 33 animals (63.46%) had at least one lesion. Theconcentric hypertrophy left ventricular was the most...

Grau de malignidade do mastocitoma cutâneo canino quanto a localização segundo as classificações de Patnaik et al. (1984) e Kiupel et al. (2011) / Degree of malignancy of the canine cutaneous mast cell tumor as forlocation according to the classifications of Patnaik et al. (1984) and Kiupel et al. (2011)

Objetivou-se com o presente trabalho coletar dados epidemiológicos relacionados com o mastocitoma canino, além de verificar se há relação entre sua malignidade e localização e, por fim, comparar os métodos de classificação histopatológica segundo Patnaik et al. (1984) e segundo Kiupel et al. (2011). Informações foram coletadas da ficha clínica de 55 cães e 60 fragmentos de pele com mastocitomas foram avaliados histologicamente. Verificou-se que a ocorrência de mastocitoma cutâneo não é influenciada pelo sexo e raça, porém cães sem raça definida e boxers são mais acometidos. Não há uma faixa etária susceptível bem definida, sendo o mastocitoma mais frequente em cães de 8 a 9 anos de idade. A região mais acometida foi a inguinal (50%) e a cabeça a região que apresentou mastocitomas com maior malignidade. Utilizando a classificação de Patnaik et al. (1984) houve diferença significativa entre as classificações histopatológicas de mastocitoma avaliadas por diferentes patologistas, o que não ocorreu utilizando a classificação de Kiupel et al. (2011). Pode-se dizer então que a classificação de Kiupel et al. (2011) gera menor divergência nos diagnósticos, demonstrando-se um método simples e eficaz para a avaliação histopatológica de mastocitoma.

The aim of the present study is to collect epidemiological data related to the canine mastocytoma, to check if there is relationbetween malignancy and its location and, finally, to compare the methods of histopathological classification according to Patnaiket al. (1984) and Kiupel et al. (2011). Informations were collected from the clinical record of 55 dogs and 60 fragments of skinwith a diagnosis of mast cell tumor were evaluated histologically. It was found that the occurrence of cutaneous mast cell tumorsis not influenced by gender and race, but dogs without defined breed and boxers are most affected. There is not a well-definedsusceptible age, but the most mast cell tumors in dogs often 8 to 9 years old. The highest affected region was inguinal (50%) andhead proved a region with higher malignancy. Using the classification Patnaik et al. (1984) there was a significant difference betweenthe histopathological ratings mastocytoma evaluated by different pathologists, which did not occur using the classification Kiupel etal. (2011). It can be said then that the classification Kiupel et al. (2011) generates less divergence in the diagnosis, demonstratinga simple and effective method for histopathological evaluation of mastocytoma.