Microbiota bacteriana do Macrobrachium amazonicum do rio São Francisco / Bacterial microbiota of the Macrobrachium amazonicum from São Francisco river, Brazil

O presente estudo teve por objetivo pesquisar a microbiota bacteriana do camarão-canela, espécie continental de freqüente ocorrência. Os camarões foram coletados nas margens do Rio São Francisco na cidade de Petrolina-PE e semeados em Tryptic Soy Agar (TSA) e Agar MacConkey (MC) e incubados a 27ºC. A leitura das placas foi realizada em até 48h e os agentes bacterianos foram identificados. Foram encontrados 26 isolados entre eles: Enterobacter spp. (n=7), Klebsiella spp. (n=4), Staphylococcus spp. (n=4), Acinetobacter spp. (n=3), 2 Streptococcus spp. (n=2), Alcaligenes spp. (n=2), Shigella spp. (n=1), Escherichia coli (n=1), Enterococcus spp. (n=1) e Pseudomonas spp. (n=1). A atividade antimicrobiana foi verificada pelo método de difusão em discos de Kirby-Bauer. Dos isolados 92,3% foram sensíveis a enrofloxacina ; 88,4% a tetraciclina ; 84,6% a ceftriaxona ; 84,6% foram sensíveis a sulfazotrim ; 76,9% a estreptomicina ; 65,3% a gentamicina ; 57,6% ao ácido nalidíxico ; 57,6% a eritromicina ; 53,8% a ampicilina ; 50% a amoxacilina ; 46,1% a nitrofurantoína ; 42,3% a neomicina e 38,4% a lincomicina . Sendo a bactéria Streptococcus spp., em média, a mais sensível aos antimicrobianos e Pseudomonas spp. menos sensível. Este estudo demonstrou que podem ser encontradas bactérias com potencial patogênico para os seres humanos na espécie M. amazonicum.

The objective of the present study was to determine the bacterial microbiota of shrimp cinnamon, continental kind of frequent occurrence. The shrimps were collected on the banks of the Rio São Francisco in the city of Petrolina-PE and streaked in Tryptic Soy Agar (TSA) and MacConkey agar (MC) and incubated at 27ºC. The reading of the plates was performed in up to 48 hours, and bacterial agents were identified. Twenty six 26 isolates were found being: Enterobacter spp. (n=7), Klebsiella spp. (n=4), Staphylococcus spp. (n=4), Acinetobacter spp. (n=3), Streptococcus spp. (n=2), Alcaligenes spp. (n=2), Shigella spp. (n=1), Escherichia coli (n=1), Enterococcus spp. (n=1), and Pseudomonas spp. (n=1). Antimicrobial activity was determined by the disk diffusion Kirby-Bauer test. Of the isolates 92.3% were sensible to enrofloxacin , 88.4% to tetracycline, 84.6% to ceftriaxone , 84.6% to sulfazotrim, 76.9% to streptomycin, 65.3% to gentamicin, 57.6% to nalidixic acid, 57.6% to erythromycin, 53.8% to ampicillin, 50% to amoxacillin, 46.1% to nitrofurantoin, 42.3% to neomycin and 38.4% to lincomycin. The more sensible bacterial genus was Streptococcus spp. and Pseudomonas spp. the least sensitive. This study showed that bacteria with pathogenic potential for man in specie M. amazonicum.

Microbiota bacteriana do Macrobrachium amazonicum do rio São Francisco / Bacterial microbiota of the Macrobrachium amazonicum from São Francisco river

O presente estudo teve por objetivo pesquisar a microbiota bacteriana do camarão-canela, espécie continental de freqüente ocorrência. Os camarões foram coletados nas margens do Rio São Francisco na cidade de Petrolina-PE e semeados em Tryptic Soy Agar (TSA) e Agar MacConkey (MC) e incubados a 27ºC. A leitura das placas foi realizada em até 48h e os agentes bacterianos foram identificados. Foram encontrados 26 isolados entre eles: Enterobacter spp. (n=7), klebsiella spp. (n=4), Staphylococcus spp. (n=4), Acinetobacter spp. (n=3), 2 Streptococcus spp. (n=2), Alcaligenes spp. (n=2), Shigella spp. (n=1), Escherichia coli (n=1), Enterococcus spp. (n=1) e Pseudomonas spp. (n=1). A atividade antimicrobiana foi verificada pelo método de difusão em discos de Kirby-Bauer. Dos isolados 92,3% foram sensíveis a enrofloxacina ; 88,4% a tetraciclina ; 84,6% a ceftriaxona ; 84,6% foram sensíveis a sulfazotrim ; 76,9% a estreptomicina ; 65,3% a gentamicina ; 57,6% ao ácido nalidíxico ; 57,6% a eritromicina ; 53,8% a ampicilina ; 50% a amoxacilina ; 46,1% a nitrofurantoína ; 42,3% a neomicina e 38,4% a lincomicina . Sendo a bactéria Streptococcus spp., em média, a mais sensível aos antimicrobianos e Pseudomonas spp. menos sensível. Este estudo demonstrou que podem ser encontradas bactérias com potencial patogênico para os seres humanos na espécie M. amazonicum.(AU)

The objective of the present study was to determine the bacterial microbiota of shrimp cinnamon, continental kind of frequent occurrence. The shrimps were collected on the banks of the Rio São Francisco in the city of Petrolina-PE and streaked in Tryptic Soy Agar (TSA) and MacConkey agar (MC) and incubated at 27ºC. The reading of the plates was performed in up to 48 hours, and bacterial agents were identified. Twenty six 26 isolates were found being: Enterobacter spp. (n=7), Klebsiella spp. (n=4), Staphylococcus spp. (n=4), Acinetobacter spp. (n=3), Streptococcus spp. (n=2), Alcaligenes spp. (n=2), Shigella spp. (n=1), Escherichia coli (n=1), Enterococcus spp. (n=1), and Pseudomonas spp. (n=1). Antimicrobial activity was determined by the disk diffusion Kirby-Bauer test. Of the isolates 92.3% were sensible to enrofloxacin , 88.4% to tetracycline, 84.6% to ceftriaxone , 84.6% to sulfazotrim, 76.9% to streptomycin, 65.3% to gentamicin, 57.6% to nalidixic acid, 57.6% to erythromycin, 53.8% to ampicillin, 50% to amoxacillin, 46.1% to nitrofurantoin, 42.3% to neomycin and 38.4% to lincomycin. The more sensible bacterial genus was Streptococcus spp. and Pseudomonas spp. The least sensitive. This study showed that bacteria with pathogenic potential for man in specie M. amazonicum.(AU)