Evaluation of microscopic protocols for somatic cell counts in milk of dairy sheep / Avaliação de protocolos do método microscópico para contagem de células somáticas no leite de ovelhas leiteiras

The somatic cell count (SCC) is a diagnostic tool that indicates the mammary gland health and can be determined by the counting of cells in the microscope. There are discussions regarding appropriate staining method to the ewes' milk. The present study aimed to identify a methodology of microscopic SCC proper to the milk of the ovine species. Therefore, glass slides for smear were manufactured with 10 µL of ewe's milk in 1 cm2, and the fixers xylol and Carnoy's solution were tested, as well as and May-Grünwald, Broadhurst-Paley, Wrigth and Panoptic stainings. Carnoy's solution was elected, because it allowed a better fixation of the dairy film to the microscope slides, and Broadhurst-Paley staining, due to its good coloration and visualization of cells, as well as the differentiation of cytoplasmic corpuscles in ewe's milk. Broadhurst-Paley coloration is a tool applicable to the somatic cell count in ovine specie's milk.(AU)

A contagem de células somáticas é uma ferramenta de diagnóstico indicativa da saúde da glândula mamária e pode ser determinada em microscópio. Sobre ela, existem discussões quanto à coloração adequada ao leite de ovelhas. O presente estudo objetivou identificar uma metodologia de preparação de lâminas para a contagem microscópica de células somáticas do leite da espécie ovina. Para tanto, confeccionaram-se lâminas de esfregaços de 10 µL de leite de ovelha em 1 cm2, testaram-se os fixadores de xilol e solução de Carnoy e as colorações de May-Grünwald, Broadhurst-Paley, Wrigth e Panótico. Elegeram-se a solução de Carnoy, pois esta permitiu melhor fixação do filme lácteo às lâminas de microscopia, e o corante Broadhurst-Paley, que propiciou boa coloração e visualização das células, bem como a diferenciação dos corpúsculos citoplasmáticos presentes no leite ovino. A coloração é uma ferramenta aplicável à contagem de células somáticas no leite da espécie ovina.(AU)

Evaluation of microscopic protocols for somatic cell counts in milk of dairy sheep / Avaliação de protocolos do método microscópico para contagem de células somáticas no leite de ovelhas leiteiras

The somatic cell count (SCC) is a diagnostic tool that indicates the mammary gland health and can be determined by the counting of cells in the microscope. There are discussions regarding appropriate staining method to the ewes' milk. The present study aimed to identify a methodology of microscopic SCC proper to the milk of the ovine species. Therefore, glass slides for smear were manufactured with 10 µL of ewe's milk in 1 cm2, and the fixers xylol and Carnoy's solution were tested, as well as and May-Grünwald, Broadhurst-Paley, Wrigth and Panoptic stainings. Carnoy's solution was elected, because it allowed a better fixation of the dairy film to the microscope slides, and Broadhurst-Paley staining, due to its good coloration and visualization of cells, as well as the differentiation of cytoplasmic corpuscles in ewe's milk. Broadhurst-Paley coloration is a tool applicable to the somatic cell count in ovine specie's milk.(AU)

A contagem de células somáticas é uma ferramenta de diagnóstico indicativa da saúde da glândula mamária e pode ser determinada em microscópio. Sobre ela, existem discussões quanto à coloração adequada ao leite de ovelhas. O presente estudo objetivou identificar uma metodologia de preparação de lâminas para a contagem microscópica de células somáticas do leite da espécie ovina. Para tanto, confeccionaram-se lâminas de esfregaços de 10 µL de leite de ovelha em 1 cm2, testaram-se os fixadores de xilol e solução de Carnoy e as colorações de May-Grünwald, Broadhurst-Paley, Wrigth e Panótico. Elegeram-se a solução de Carnoy, pois esta permitiu melhor fixação do filme lácteo às lâminas de microscopia, e o corante Broadhurst-Paley, que propiciou boa coloração e visualização das células, bem como a diferenciação dos corpúsculos citoplasmáticos presentes no leite ovino. A coloração é uma ferramenta aplicável à contagem de células somáticas no leite da espécie ovina.(AU)

Somatic cell count and California Mastitis Test as a diagnostic tool for subclinical mastitis in ewes

Background: Infectious mastitis has been described as one of the main diseases affecting animals during lactation. The disease in sheep has been studied for many years in countries where mastitis has an economical importance. However, the interest in mastitis studies in animals raised for food production has increased, because the disease may cause a reduction in weight and an increased mortality in lambs. In this study, Somatic Cells Count (SCC) and California Mastitis Test (CMT) were related to bacterial isolation for mastitis diagnosis in Corriedale sheep. Material, Methods & Results: Twenty nine (29) ewes, varying from 2-5 years of age, with different numbers of lactation and born lambs and never before machine-milked were used. Milking was done from October to November, once a week in the morning period, with oxytocin application. Four milk collections were made, at biweekly intervals for bacteriologic, SCC and CMT test, which were analyzed considering each gland as a sampling unit. Low incidence of subclinical mastitis (9.66%) was observed, with the majority (15/17) caused by coagulase negative Staphylococcus. There was no change in milk production related to SCC or bacteria isolation. However changes in milk components occurred in the presence of subclinical mastitis. A poor relation (k = 0.115) was determined between the results obtained in the bacteriological test and the SCC, with low sensitivity (13.33%) and an increased number of false negative results (13%). Comparing the SCC and CMT results as the diagnostic method for subclinical mastitis, a low (r = 0.2319) but significant (P = 0.0209) correlation was observed, as well as a poor concordance (k = 0.152). Using bacterial isolation as the standard test, it was determined that the CMT has low sensitivity (28.57%) as the diagnostic method of mastitis in ewes. Discussion: Mastitis has been considered an economically important disease in the production of sheep for meat and wool. According to reports, the frequency of its clinical occurrence may range from zero to 50%. In meat-producing herds, a low weight gain in lambs has been associated with subclinical mastitis and the study of mastitis in Corriedale sheep is justified since this is considered a breed of meat sheep with the best milk production. This breed is being crossed with milkproducing breeds, such as Laucane, to form milk-producing herds. Bacterial isolation has been adopted as the diagnostic method of mastitis in all livestock breeds. Similarly to the observation made by this study, Staphylococcus and, in a few cases, Streptococcus, have been the microorganisms most frequently involved in subclinical mastitis in sheep. The milk from ewes with mastitis tends to have a lower fat and lactose content than that of healthy ewes, due to the affected secretory function of these animals. The SCC of milk ewes has not yet been established, but its count in a healthy udder may reach up to 1.5x106 cel.mL-¹. Similarly, the CMT score to be used in sheep is still controversial, but the maximum score (+++) is adopted to indicate mastitis. The high number of false-negative and false-positive reactions observed in SCC and CMT tests means that healthy and ill animals are incorrectly identified and that no preventive and curative measures are adopted. Since the utilization of only one diagnosis method in sheep mastitis, without confirmation by bacteriologic test is not conclusive, the SCC and CMT should be used cautiously in sheep mastitis diagnosis.

Somatic cell count and california mastitis test as a diagnostic tool for subclinical mastitis in ewes / Somatic cell count and california mastitis test as a diagnostic tool for subclinical mastitis in ewes

Background: Infectious mastitis has been described as one of the main diseases affecting animals during lactation. The disease in sheep has been studied for many years in countries where mastitis has an economical importance. However, the interest in mastitis studies in animals raised for food production has increased, because the disease may cause a reduction in weight and an increased mortality in lambs. In this study, Somatic Cells Count (SCC) and California Mastitis Test (CMT) were related to bacterial isolation for mastitis diagnosis in Corriedale sheep.Material, Methods & Results: Twenty nine (29) ewes, varying from 2-5 years of age, with different numbers of lactation and born lambs and never before machine-milked were used. Milking was done from October to November, once a week in the morning period, with oxytocin application. Four milk collections were made, at biweekly intervals for bacteriologic, SCC and CMT test, which were analyzed considering each gland as a sampling unit. Low incidence of subclinical mastitis (9.66%) was observed, with the majority (15/17) caused by coagulase negative Staphylococcus. There was no change in milk production related to SCC or bacteria isolation. However changes in milk components occurred in the presence of subclinical mastitis. A poor relation (k = 0.115) was determined between the results obtained in the bacteriological test a

Background: Infectious mastitis has been described as one of the main diseases affecting animals during lactation. The disease in sheep has been studied for many years in countries where mastitis has an economical importance. However, the interest in mastitis studies in animals raised for food production has increased, because the disease may cause a reduction in weight and an increased mortality in lambs. In this study, Somatic Cells Count (SCC) and California Mastitis Test (CMT) were related to bacterial isolation for mastitis diagnosis in Corriedale sheep.Material, Methods & Results: Twenty nine (29) ewes, varying from 2-5 years of age, with different numbers of lactation and born lambs and never before machine-milked were used. Milking was done from October to November, once a week in the morning period, with oxytocin application. Four milk collections were made, at biweekly intervals for bacteriologic, SCC and CMT test, which were analyzed considering each gland as a sampling unit. Low incidence of subclinical mastitis (9.66%) was observed, with the majority (15/17) caused by coagulase negative Staphylococcus. There was no change in milk production related to SCC or bacteria isolation. However changes in milk components occurred in the presence of subclinical mastitis. A poor relation (k = 0.115) was determined between the results obtained in the bacteriological test a

Presença de Salmonella spp. em pintos de um dia, comercializados para produção não industrial em Santa Catarina / Presence of Salmonella spp. in day-old chicks commercialized for non-industrial production in Santa Catarina

Samonella sp. is a microorganism that can infect a great range of animals, including human beings. In the environment Salmonella spp. become an important source of transmission and, consequently, a risk to animal production and public health. In Brazil, the control and certification of poultry establishment as free of Salmonella spp. was established in 2003. The Gallinarum, Pullorum, Enteritidis and Typhimurium serotypes are monitored by the Plano Nacional de Sanidade Avícola (Brazilian National Poultry Health Program - PNSA). The Gallinarum and Pullorum serotypes are important in poultry health, and their presence in layer farms may cause a great economic impact, while Enteritidis and Typhimurium are among the most frequently isolated serovars in cases of food poisoning (DTA), where food of poultry origin stands as the main source of salmonellosis such as eggs or salad prepared with homemade mayonnaise or even chicken. Salmonella spp. is one of the most important contaminants of food products and an important cause of food borne disease in many countries. On the other hand, broiler chick or laying hens are increasingly being produced in non-industrial conditions. Such productive units normally donot adopt biosafety criteria or sanitary programs and, consequently, they may contribute to the dissemination of salmonelas. Therefore, the objective of this study was to verify the presence of Salmonella spp. in day-old chicks commercialized for nonindustrial production in the State of Santa Catarina.[...]

Presença de Salmonella spp. em pintos de um dia, comercializados para produção não industrial em Santa Catarina / Presence of Salmonella spp. in day-old chicks commercialized for non-industrial production in Santa Catarina

Samonella sp. is a microorganism that can infect a great range of animals, including human beings. In the environment Salmonella spp. become an important source of transmission and, consequently, a risk to animal production and public health. In Brazil, the control and certification of poultry establishment as free of Salmonella spp. was established in 2003. The Gallinarum, Pullorum, Enteritidis and Typhimurium serotypes are monitored by the Plano Nacional de Sanidade Avícola (Brazilian National Poultry Health Program - PNSA). The Gallinarum and Pullorum serotypes are important in poultry health, and their presence in layer farms may cause a great economic impact, while Enteritidis and Typhimurium are among the most frequently isolated serovars in cases of food poisoning (DTA), where food of poultry origin stands as the main source of salmonellosis such as eggs or salad prepared with homemade mayonnaise or even chicken. Salmonella spp. is one of the most important contaminants of food products and an important cause of food borne disease in many countries. On the other hand, broiler chick or laying hens are increasingly being produced in non-industrial conditions. Such productive units normally donot adopt biosafety criteria or sanitary programs and, consequently, they may contribute to the dissemination of salmonelas. Therefore, the objective of this study was to verify the presence of Salmonella spp. in day-old chicks commercialized for nonindustrial production in the State of Santa Catarina.[...](AU)

Atividade antimicrobiana dos óleos essenciais de orégano, tomilho e canela frente a sorovares de Salmonella enterica de origem avícola

Essential oils from oregano (Origanum vulgare), thyme (Thymus vulgaris) and cinnamom (Cinnamomum zeylanicum) were tested for their inhibitory activity against sixty Salmonella sp. isolates. These microorganisms were distributed in 20 serovars, all of them isolated from broiler chickens. Based on microdilution technique, the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined. The strongest antibacterial activity was obtained for oregano (MIC mean = 529mug ml-1 and MBCmean = 661mug ml-1), followed by a moderate activity for thyme (MICmean=961mug ml-1 and MBCmean=1074mug ml-1) and the lowest obtained activity observed for cinnamon (MICmean= 1335mug ml-1 and MBCmean= 1979mug ml-1). Despite of this activity pattern, the serovars of Salmonella enterica showed significant susceptibility variations. However none of them could be classified as sensitive or resistant to the three essential oils tested. At the moment, when the antibiotics, as growth promoters, are being replaced, the essential oils from spices could be a very reasonable choice and the present results show us two points: the essential oils from oregano and thyme are effective against Salmonella isolates but the variations among the serovars must be considered.

Avaliou-se a atividade antimicrobiana dos óleos essenciais de orégano (Origanum vulgare), tomilho (Thymus vulgaris) e canela (Cinnamomum zeylanicum) frente a 60 amostras de Salmonella enterica distribuídas entre 20 sorovares, todos isolados de carcaças de aves. A concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM) foram determinadas para cada isolado, utilizando-se a técnica de microdiluição em caldo. O óleo essencial de orégano evidenciou forte atividade antibacteriana (CIM média = 529mig ml-1 e CBM média= 661mig ml-1), seguido do tomilho (CIM média=961mig ml-1 e CBM média= 1074mig ml-1), com atividade moderada, enquanto que a menor atividade foi observada com o óleo essencial de canela (CIM média= 1335mig ml-1 e CBM média = 1979mig ml-1). Apesar deste claro padrão de atividade, os sorovares de Salmonella enterica evidenciaram significativas variações de suscetibilidade, embora nenhuma, em particular, pudesse ser classificada como totalmente sensível ou resistente aos três óleos essenciais avaliados. Assim, no momento em que os antibacterianos utilizados como promotores de crescimento estão sendo substituídos. E os óleos essenciais de temperos e condimentos se constituem numa alternativa importante, os resultados aqui apresentados sinalizam dois pontos: os óleos essenciais de orégano e tomilho são efetivos contra Salmonella, mas as variações de suscetibilidade entre os sorovares deverão também ser consideradas.

Atividade antimicrobiana dos óleos essenciais de orégano, tomilho e canela frente a sorovares de Salmonella enterica de origem avícola

Essential oils from oregano (Origanum vulgare), thyme (Thymus vulgaris) and cinnamom (Cinnamomum zeylanicum) were tested for their inhibitory activity against sixty Salmonella sp. isolates. These microorganisms were distributed in 20 serovars, all of them isolated from broiler chickens. Based on microdilution technique, the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined. The strongest antibacterial activity was obtained for oregano (MIC mean = 529mug ml-1 and MBCmean = 661mug ml-1), followed by a moderate activity for thyme (MICmean=961mug ml-1 and MBCmean=1074mug ml-1) and the lowest obtained activity observed for cinnamon (MICmean= 1335mug ml-1 and MBCmean= 1979mug ml-1). Despite of this activity pattern, the serovars of Salmonella enterica showed significant susceptibility variations. However none of them could be classified as sensitive or resistant to the three essential oils tested. At the moment, when the antibiotics, as growth promoters, are being replaced, the essential oils from spices could be a very reasonable choice and the present results show us two points: the essential oils from oregano and thyme are effective against Salmonella isolates but the variations among the serovars must be considered.

Avaliou-se a atividade antimicrobiana dos óleos essenciais de orégano (Origanum vulgare), tomilho (Thymus vulgaris) e canela (Cinnamomum zeylanicum) frente a 60 amostras de Salmonella enterica distribuídas entre 20 sorovares, todos isolados de carcaças de aves. A concentração inibitória mínima (CIM) e a concentração bactericida mínima (CBM) foram determinadas para cada isolado, utilizando-se a técnica de microdiluição em caldo. O óleo essencial de orégano evidenciou forte atividade antibacteriana (CIM média = 529mig ml-1 e CBM média= 661mig ml-1), seguido do tomilho (CIM média=961mig ml-1 e CBM média= 1074mig ml-1), com atividade moderada, enquanto que a menor atividade foi observada com o óleo essencial de canela (CIM média= 1335mig ml-1 e CBM média = 1979mig ml-1). Apesar deste claro padrão de atividade, os sorovares de Salmonella enterica evidenciaram significativas variações de suscetibilidade, embora nenhuma, em particular, pudesse ser classificada como totalmente sensível ou resistente aos três óleos essenciais avaliados. Assim, no momento em que os antibacterianos utilizados como promotores de crescimento estão sendo substituídos. E os óleos essenciais de temperos e condimentos se constituem numa alternativa importante, os resultados aqui apresentados sinalizam dois pontos: os óleos essenciais de orégano e tomilho são efetivos contra Salmonella, mas as variações de suscetibilidade entre os sorovares deverão também ser consideradas.

Qualidade microbiológica de leite cru produzido em cinco municípios do Estado do Rio Grande do Sul, Brasil

O leite pode ser considerado um bom meio de cultura para o crescimento de microrganismos. Estes microrganismos podem contaminar o homem através da ingestão de leite cru, influenciar no tipo de processamento a ser utilizado e interferir na qualidade deste produto e seus derivados. Propriedades leiteiras de cinco municípios no estado do Rio Grande do Sul, Brasil, foram examinadas para determinar a qualidade microbiológica do leite produzido. Foi verificada a presença de coliformes totais, fecais e Salmonella sp. Foi realizada, também, a contagem de bactérias mesófilas, psicrotróficas e de leveduras. Dentre 42 propriedades, 41 apresentaram coliformes totais no leite. Não foi observada diferença significativa entre os tipos de ordenha e os diferentes métodos de resfriamento durante o armazenamento. Somente oito propriedades apresentaram coliformes fecais e confirmação de Escherichia coli, evidenciando falhas nos procedimentos higiênicos durante a ordenha ou armazenamento. Não foi detectada a presença de Salmonella sp. nas amostras, confirmando sua baixa prevalência no leite. Todas as 12 propriedades testadas quanto à presença de bactérias mesófilas e psicrotróficas apresentaram contagens superiores ou igual a 4 log.ufc.mL-1. Foi observada a presença de leveduras em 32 das 34 propriedades testadas com contagens variando entre 1,52 e acima de 4 log.ufc.mL-1. O número elevado de micro

Somatic cell count and california mastitis test as a diagnostic tool for subclinical mastitis in ewes / Somatic cell count and california mastitis test as a diagnostic tool for subclinical mastitis in ewes

Background: Infectious mastitis has been described as one of the main diseases affecting animals during lactation. The disease in sheep has been studied for many years in countries where mastitis has an economical importance. However, the interest in mastitis studies in animals raised for food production has increased, because the disease may cause a reduction in weight and an increased mortality in lambs. In this study, Somatic Cells Count (SCC) and California Mastitis Test (CMT) were related to bacterial isolation for mastitis diagnosis in Corriedale sheep.Material, Methods & Results: Twenty nine (29) ewes, varying from 2-5 years of age, with different numbers of lactation and born lambs and never before machine-milked were used. Milking was done from October to November, once a week in the morning period, with oxytocin application. Four milk collections were made, at biweekly intervals for bacteriologic, SCC and CMT test, which were analyzed considering each gland as a sampling unit. Low incidence of subclinical mastitis (9.66%) was observed, with the majority (15/17) caused by coagulase negative Staphylococcus. There was no change in milk production related to SCC or bacteria isolation. However changes in milk components occurred in the presence of subclinical mastitis. A poor relation (k = 0.115) was determined between the results obtained in the bacteriological test a

Background: Infectious mastitis has been described as one of the main diseases affecting animals during lactation. The disease in sheep has been studied for many years in countries where mastitis has an economical importance. However, the interest in mastitis studies in animals raised for food production has increased, because the disease may cause a reduction in weight and an increased mortality in lambs. In this study, Somatic Cells Count (SCC) and California Mastitis Test (CMT) were related to bacterial isolation for mastitis diagnosis in Corriedale sheep.Material, Methods & Results: Twenty nine (29) ewes, varying from 2-5 years of age, with different numbers of lactation and born lambs and never before machine-milked were used. Milking was done from October to November, once a week in the morning period, with oxytocin application. Four milk collections were made, at biweekly intervals for bacteriologic, SCC and CMT test, which were analyzed considering each gland as a sampling unit. Low incidence of subclinical mastitis (9.66%) was observed, with the majority (15/17) caused by coagulase negative Staphylococcus. There was no change in milk production related to SCC or bacteria isolation. However changes in milk components occurred in the presence of subclinical mastitis. A poor relation (k = 0.115) was determined between the results obtained in the bacteriological test a

Qualidade microbiológica de leite cru produzido em cinco municípios do Estado do Rio Grande do Sul, Brasil

O leite pode ser considerado um bom meio de cultura para o crescimento de microrganismos. Estes microrganismos podem contaminar o homem através da ingestão de leite cru, influenciar no tipo de processamento a ser utilizado e interferir na qualidade deste produto e seus derivados. Propriedades leiteiras de cinco municípios no estado do Rio Grande do Sul, Brasil, foram examinadas para determinar a qualidade microbiológica do leite produzido. Foi verificada a presença de coliformes totais, fecais e Salmonella sp. Foi realizada, também, a contagem de bactérias mesófilas, psicrotróficas e de leveduras. Dentre 42 propriedades, 41 apresentaram coliformes totais no leite. Não foi observada diferença significativa entre os tipos de ordenha e os diferentes métodos de resfriamento durante o armazenamento. Somente oito propriedades apresentaram coliformes fecais e confirmação de Escherichia coli, evidenciando falhas nos procedimentos higiênicos durante a ordenha ou armazenamento. Não foi detectada a presença de Salmonella sp. nas amostras, confirmando sua baixa prevalência no leite. Todas as 12 propriedades testadas quanto à presença de bactérias mesófilas e psicrotróficas apresentaram contagens superiores ou igual a 4 log.ufc.mL-1. Foi observada a presença de leveduras em 32 das 34 propriedades testadas com contagens variando entre 1,52 e acima de 4 log.ufc.mL-1. O número elevado de micro

Qualidade microbiológica de leite cru produzido em cinco municípios do Estado do Rio Grande do Sul, Brasil

O leite pode ser considerado um bom meio de cultura para o crescimento de microrganismos. Estes microrganismos podem contaminar o homem através da ingestão de leite cru, influenciar no tipo de processamento a ser utilizado e interferir na qualidade deste produto e seus derivados. Propriedades leiteiras de cinco municípios no estado do Rio Grande do Sul, Brasil, foram examinadas para determinar a qualidade microbiológica do leite produzido. Foi verificada a presença de coliformes totais, fecais e Salmonella sp. Foi realizada, também, a contagem de bactérias mesófilas, psicrotróficas e de leveduras. Dentre 42 propriedades, 41 apresentaram coliformes totais no leite. Não foi observada diferença significativa entre os tipos de ordenha e os diferentes métodos de resfriamento durante o armazenamento. Somente oito propriedades apresentaram coliformes fecais e confirmação de Escherichia coli, evidenciando falhas nos procedimentos higiênicos durante a ordenha ou armazenamento. Não foi detectada a presença de Salmonella sp. nas amostras, confirmando sua baixa prevalência no leite. Todas as 12 propriedades testadas quanto à presença de bactérias mesófilas e psicrotróficas apresentaram contagens superiores ou igual a 4 log.ufc.mL-1. Foi observada a presença de leveduras em 32 das 34 propriedades testadas com contagens variando entre 1,52 e acima de 4 log.ufc.mL-1. O número elevado de micro

Qualidade bacteriológica de leite bovino de mistura, in natura e beneficiado, e detecção sorológica de Brucelose em rebanhos da Região Metropolitana de Porto Alegre

O leite é uma fonte excelente de nutrientes tornando-se um meio de cultura ideal para o crescimento de microrganismos potencialmente patogênicos. Estes microrganismos podem comprometer a qualidade e segurança do leite e seus derivados e podem contaminar o homem através da ingestão do leite in natura ou beneficiado contaminados. Para estimar a qualidade microbiológica do leite in natura, foram coletadas 116 amostras em 42 propriedades leiteiras nos municípios de Esteio, Glorinha, Gravataí, Sapucaia do Sul e Viamão, no Estado do Rio Grande do Sul, Brasil. A presença de coliformes fecais e E. coli foi confirmada em 24 isolados, referentes a oito propriedades. Neste trabalho não foi possível detectar a presença de Salmonella sp. As amostras testadas estavam dentro dos padrões exigidos pela legislação para microrganismos mesófilos e psicrotróficos, porém alguns autores citam que contagens acima de 3 log.ufc.ml-1 podem ser prejudiciais ao processamento do produto. Para detecção de anticorpos específicos de Brucella sp. foi utilizado o Teste de Anel de Leite, em 181 amostras (116 amostras coletadas acrescidas de 65 fornecidas pela indústria de beneficiamento), sendo encontradas dez amostras positivas em três propriedades. A qualidade higiênico-sanitária nas propriedades analisadas demostrou ser adequada, porém foi evidenciada a presença microrganismos patogênicos em algumas amostras representando um risco à saúde pública